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Volumn 76, Issue 7, 2010, Pages 2098-2106

Development and application of a PCR-targeted gene disruption method for studying CeIR function in thermobifida fusca

Author keywords

[No Author keywords available]

Indexed keywords

CELL YIELDS; CELLOBIOSE; CELLULASE ACTIVITY; CELLULASE GENES; ENDOGLUCANASES; GENE DISRUPTIONS; GROWTH BEHAVIOR; INVERTED REPEAT; LAG PHASE; THERMOBIFIDA FUSCA; TRANSCRIPT LEVEL; WILD-TYPE STRAIN;

EID: 77950225539     PISSN: 00992240     EISSN: 10985336     Source Type: Journal    
DOI: 10.1128/AEM.02626-09     Document Type: Article
Times cited : (21)

References (33)
  • 1
    • 0037182342 scopus 로고    scopus 로고
    • Mutation and expression of N233C-D506C of cellulase Cel6B from Thermobifida fusca in Escherichia coli
    • Ai, Y. C., and D. B. Wilson. 2002 Mutation and expression of N233C-D506C of cellulase Cel6B from Thermobifida fusca in Escherichia coli. Enzyme Microb. Technol. 30:804-808.
    • (2002) Enzyme Microb. Technol. , vol.30 , pp. 804-808
    • Ai, Y.C.1    Wilson, D.B.2
  • 2
    • 0025886996 scopus 로고
    • Purification and cooperative activity of enzymes constituting the xylan-degrading system of Thermomonospora fusca
    • Bachmann, S. L., and A. J. McCarthy. 1991 Purification and cooperative activity of enzymes constituting the xylan-degrading system of Thermomonospora fusca. Appl. Environ. Microbiol. 57:2121-2130.
    • (1991) Appl. Environ. Microbiol. , vol.57 , pp. 2121-2130
    • Bachmann, S.L.1    McCarthy, A.J.2
  • 3
    • 0242584463 scopus 로고    scopus 로고
    • Cloning and heterologous expression of a beta-D-mannosidase (EC 3.2.1.25)-encoding gene from Thermobifida fusca TM51
    • Beki, E., S. Nagy, J. Vanderleyden, S. Jager, L. Kiss, L. Fulop, L. Hornok, and J. Kukolya. 2003 Cloning and heterologous expression of a beta-D-mannosidase (EC 3.2.1.25)-encoding gene from Thermobifida fusca TM51. Appl. Environ. Microbiol. 69:1944-1952.
    • (2003) Appl. Environ. Microbiol. , vol.69 , pp. 1944-1952
    • Beki, E.1    Nagy, S.2    Vanderleyden, J.3    Jager, S.4    Kiss, L.5    Fulop, L.6    Hornok, L.7    Kukolya, J.8
  • 4
    • 0026645203 scopus 로고
    • Plasmid cloning vectors for the conjugal transfer of DNA from Escherichia coli to Streptomyces spp
    • Bierman, M., R. Logan, K. O'Brien, E. T. Seno, R. N. Rao, and B. E. Schoner. 1992 Plasmid cloning vectors for the conjugal transfer of DNA from Escherichia coli to Streptomyces spp. Gene 116:43-49.
    • (1992) Gene , vol.116 , pp. 43-49
    • Bierman, M.1    Logan, R.2    O'Brien, K.3    Seno, E.T.4    Rao, R.N.5    Schoner, B.E.6
  • 5
    • 0017184389 scopus 로고
    • Rapid and sensitive method for quantitation of microgram quantities of protein utilizing the principal of protein-dye binding
    • Bradford, M. M. 1976 Rapid and sensitive method for quantitation of microgram quantities of protein utilizing the principal of protein-dye binding. Anal. Biochem. 72:248-254.
    • (1976) Anal. Biochem. , vol.72 , pp. 248-254
    • Bradford, M.M.1
  • 6
    • 34548496685 scopus 로고    scopus 로고
    • Froteomic and transcriptomic analysis of extracellular proteins and mRNA levels in Thermobifida fusca grown on cellobiose and glucose
    • Chen, S. L, and D. B. Wilson. 2007 Froteomic and transcriptomic analysis of extracellular proteins and mRNA levels in Thermobifida fusca grown on cellobiose and glucose. J. Bacteriol. 189:6260-6265.
    • (2007) J. Bacteriol. , vol.189 , pp. 6260-6265
    • Chen, S.L.1    Wilson, D.B.2
  • 7
    • 0034612342 scopus 로고    scopus 로고
    • One-step inactivation of chromosomal genes in Escherichia coli K-12 using FCR products
    • Datsenko, K. A., and B. L. Wanner. 2000 One-step inactivation of chromosomal genes in Escherichia coli K-12 using FCR products. Proc. Natl. Acad. Sci. U. S. A. 97:6640-6645.
    • (2000) Proc. Natl. Acad. Sci. U. S. A. , vol.97 , pp. 6640-6645
    • Datsenko, K.A.1    Wanner, B.L.2
  • 8
    • 76649098306 scopus 로고    scopus 로고
    • Influence of culture aeration on the cellulase activity of Thermobifida fusca
    • Deng, Y., and S. S. Fong. 2009 Influence of culture aeration on the cellulase activity of Thermobifida fusca. Appl. Microbiol. Biotechnol. 85:965-974.
    • (2009) Appl. Microbiol. Biotechnol. , vol.85 , pp. 965-974
    • Deng, Y.1    Fong, S.S.2
  • 9
    • 0345730809 scopus 로고    scopus 로고
    • Ruminococcus albus 8 mutants defective in cellulose degradation are deficient in two processive endocellulases, Cel48A and Cel9B, both of which possess a novel modular architecture
    • Devillard, E., D. B. Goodheart, S. K. R. Karnati, E. A. Bayer, R. Lamed, J. Miron, K. E. Nelson, and M. Morrison. 2004 Ruminococcus albus 8 mutants defective in cellulose degradation are deficient in two processive endocellulases, Cel48A and Cel9B, both of which possess a novel modular architecture. J. Bacteriol. 186:136-145.
    • (2004) J. Bacteriol. , vol.186 , pp. 136-145
    • Devillard, E.1    Goodheart, D.B.2    Karnati, S.K.R.3    Bayer, E.A.4    Lamed, R.5    Miron, J.6    Nelson, K.E.7    Morrison, M.8
  • 10
    • 0033104145 scopus 로고    scopus 로고
    • Gene replacement with linear DNA in electroporated wild-type Escherichia coli
    • El Karoui, M., S. K. Amundsen, P. Dabert, and A. Grass. 1999 Gene replacement with linear DNA in electroporated wild-type Escherichia coli. Nucleic Acids Res. 27:1296-1299.
    • (1999) Nucleic Acids Res. , vol.27 , pp. 1296-1299
    • El Karoui, M.1    Amundsen, S.K.2    Dabert, P.3    Grass, A.4
  • 11
    • 0021054302 scopus 로고
    • Effect of cellobiose, glucose, ethanol, and metal-ions on the cellulase enzyme complex of Thermomonospora fusca
    • Ferchak, J. D., and E. K. Pye. 1983 Effect of cellobiose, glucose, ethanol, and metal-ions on the cellulase enzyme complex of Thermomonospora fusca. Biotechnol. Bioeng. 25:2865-2872.
    • (1983) Biotechnol. Bioeng. , vol.25 , pp. 2865-2872
    • Ferchak, J.D.1    Pye, E.K.2
  • 12
    • 0037452723 scopus 로고    scopus 로고
    • PCR-targeted Streptomyces gene replacement identifies a protein domain needed for biosynthesis of the sesquiterpene soil odor geosmin
    • Gust, B., G. L. Challis, K. Fowler, T. Kieser, and K. F. Chater. 2003 PCR-targeted Streptomyces gene replacement identifies a protein domain needed for biosynthesis of the sesquiterpene soil odor geosmin. Proc. Natl. Acad. Sci. U. S. A. 100:1541-1546.
    • (2003) Proc. Natl. Acad. Sci. U. S. A. , vol.100 , pp. 1541-1546
    • Gust, B.1    Challis, G.L.2    Fowler, K.3    Kieser, T.4    Chater, K.F.5
  • 13
    • 0033869510 scopus 로고    scopus 로고
    • Cloning, expression and characterization of a family 48 exocellulase, Cel48A, from Thermobifida fusca
    • Irwin, D. C., S. Zhang, and D. B. Wilson. 2000 Cloning, expression and characterization of a family 48 exocellulase, Cel48A, from Thermobifida fusca. Eur. J. Biochem. 267:4988-4997.
    • (2000) Eur. J. Biochem. , vol.267 , pp. 4988-4997
    • Irwin, D.C.1    Zhang, S.2    Wilson, D.B.3
  • 14
    • 0141504945 scopus 로고    scopus 로고
    • Binding and reversibility of Thermobifida fusca Cel5A, Cel6B, and Cel48A and their respective catalytic domains to bacterial microctystalline cellulose
    • Jung, H., D. B. Wilson, and L. P. Walker. 2003 Binding and reversibility of Thermobifida fusca Cel5A, Cel6B, and Cel48A and their respective catalytic domains to bacterial microctystalline cellulose. Biotechnol. Bioeng. 84:151-159.
    • (2003) Biotechnol. Bioeng. , vol.84 , pp. 151-159
    • Jung, H.1    Wilson, D.B.2    Walker, L.P.3
  • 15
    • 14344251903 scopus 로고    scopus 로고
    • Purification and characterization of Thermobifida fusca xylanase 10B
    • Kim, J. H., D. Irwin, and D. B. Wilson. 2004 Purification and characterization of Thermobifida fusca xylanase 10B. Can. J. Microbiol. 50:835-843.
    • (2004) Can. J. Microbiol. , vol.50 , pp. 835-843
    • Kim, J.H.1    Irwin, D.2    Wilson, D.B.3
  • 16
    • 34249740743 scopus 로고    scopus 로고
    • Processivity, substrate binding, and mechanism of cellulose hydrolysis by Thermobifida fusca Cel 9A
    • Li, Y. C., D. C. Irwin, and D. B. Wilson. 2007 Processivity, substrate binding, and mechanism of cellulose hydrolysis by Thermobifida fusca Cel 9A. Appl. Environ. Microbiol. 73:3165-3172.
    • (2007) Appl. Environ. Microbiol. , vol.73 , pp. 3165-3172
    • Li, Y.C.1    Irwin, D.C.2    Wilson, D.B.3
  • 17
    • 0342367772 scopus 로고
    • Isolation of a thermostable enzyme variant by cloning and selection in a thermophile
    • Liao, H., T. McKenzie, and R. Hageman. 1986 Isolation of a thermostable enzyme variant by cloning and selection in a thermophile. Proc. Natl. Acad. Sci. U. S. A. 83:576-580.
    • (1986) Proc. Natl. Acad. Sci. U. S. A. , vol.83 , pp. 576-580
    • Liao, H.1    McKenzie, T.2    Hageman, R.3
  • 19
    • 48649104231 scopus 로고    scopus 로고
    • Regulation and characterization of Thermobifida fusca carbohydrate-binding module proteins E7 and E8
    • Moser, F., D. C. Irwin, S. Chen, and D. B. Wilson. 2008 Regulation and characterization of Thermobifida fusca carbohydrate-binding module proteins E7 and E8. Biotechnol. Bioeng. 100:1066-1077.
    • (2008) Biotechnol. Bioeng. , vol.100 , pp. 1066-1077
    • Moser, F.1    Irwin, D.C.2    Chen, S.3    Wilson, D.B.4
  • 20
    • 0034603783 scopus 로고    scopus 로고
    • FCR-mediated gene replacement in Escherichia coli
    • Murphy, K. C., K. G. Campellone, and A. R. Poteete. 2000 FCR-mediated gene replacement in Escherichia coli. Gene 246:321-330.
    • (2000) Gene , vol.246 , pp. 321-330
    • Murphy, K.C.1    Campellone, K.G.2    Poteete, A.R.3
  • 22
    • 0022382865 scopus 로고
    • Genetic recombination and transformation in protoplasts of Thermomonospora fusca
    • Pidcock, K. A., B. S. Montenecourt, and J. A. Sands. 1985 Genetic recombination and transformation in protoplasts of Thermomonospora fusca. Appl. Environ. Microbiol. 50:693-695.
    • (1985) Appl. Environ. Microbiol. , vol.50 , pp. 693-695
    • Pidcock, K.A.1    Montenecourt, B.S.2    Sands, J.A.3
  • 23
    • 0033988339 scopus 로고    scopus 로고
    • A celR mutation affecting transcription of cellulase genes in Thermobifida fusca
    • Spiridonov, N. A., and D. B. Wilson. 2000 A celR mutation affecting transcription of cellulase genes in Thermobifida fusca. J. Bacteriol. 182:252-255.
    • (2000) J. Bacteriol. , vol.182 , pp. 252-255
    • Spiridonov, N.A.1    Wilson, D.B.2
  • 24
    • 0033531941 scopus 로고    scopus 로고
    • Characterization and cloning of CelR, a transcriptional regulator of cellulase genes from Thermomonospora fusca
    • Spiridonov, N. A., and D. B. Wilson. 1999 Characterization and cloning of CelR, a transcriptional regulator of cellulase genes from Thermomonospora fusca. J. Biol. Chem. 274:13127-13132.
    • (1999) J. Biol. Chem. , vol.274 , pp. 13127-13132
    • Spiridonov, N.A.1    Wilson, D.B.2
  • 25
    • 9244240974 scopus 로고    scopus 로고
    • Consecutive gene deletions in Mycobacterium smegmatis using the yeast FLP recombinase
    • Stephan, J., V. Stemmer, and M. Niederweis. 2004 Consecutive gene deletions in Mycobacterium smegmatis using the yeast FLP recombinase. Gene 343:181-190.
    • (2004) Gene , vol.343 , pp. 181-190
    • Stephan, J.1    Stemmer, V.2    Niederweis, M.3
  • 27
    • 0036092426 scopus 로고    scopus 로고
    • Synergism in binary mixtures of Thermobifida fusca cellulases Cel6B, Cel9A, and Cel5A on BMCC and Avicel
    • Watson, D. L, D. B. Wilson, and L. P. Walker. 2002 Synergism in binary mixtures of Thermobifida fusca cellulases Cel6B, Cel9A, and Cel5A on BMCC and Avicel. Appl. Biochem. Biotechnol. 101:97-111.
    • (2002) Appl. Biochem. Biotechnol. , vol.101 , pp. 97-111
    • Watson, D.L.1    Wilson, D.B.2    Walker, L.P.3
  • 28
    • 4143090473 scopus 로고    scopus 로고
    • Studies of Thermobifida fusca plant cell wall degrading enzymes
    • Wilson, D. B. 2004 Studies of Thermobifida fusca plant cell wall degrading enzymes. Chem. Rec. 4:72-82.
    • (2004) Chem. Rec. , vol.4 , pp. 72-82
    • Wilson, D.B.1
  • 29
    • 10844228836 scopus 로고    scopus 로고
    • Microplate-based filter paper assay to measure total cellulase activity
    • Xiao, Z., R. Storms, and A. Tsang. 2004 Microplate-based filter paper assay to measure total cellulase activity. Biotechnol. Bioeng. 88:832-837.
    • (2004) Biotechnol. Bioeng. , vol.88 , pp. 832-837
    • Xiao, Z.1    Storms, R.2    Tsang, A.3
  • 30
    • 34249869579 scopus 로고    scopus 로고
    • Production of xylooligosac-charides from xylans by extracellular xylanases from Thermobifida fusca
    • Yang, C. H., S. F. Yang, and W. H. Liu. 2007 Production of xylooligosac-charides from xylans by extracellular xylanases from Thermobifida fusca. J. Agrie. Food Chem. 55:3955-3959.
    • (2007) J. Agrie. Food Chem. , vol.55 , pp. 3955-3959
    • Yang, C.H.1    Yang, S.F.2    Liu, W.H.3
  • 31
    • 0042662854 scopus 로고    scopus 로고
    • Combination of overlapping bacterial artificial chromosomes by a two-step recombinogenic engineering method
    • Zhang, X., and J. Huang. 2003 Combination of overlapping bacterial artificial chromosomes by a two-step recombinogenic engineering method. Nucleic Acids Res. 31:e81.
    • (2003) Nucleic Acids Res. , vol.31
    • Zhang, X.1    Huang, J.2
  • 32
    • 0033668496 scopus 로고    scopus 로고
    • DNA cloning by homologous recombination in Escherichia coli
    • Zhang, Y., J. P. Muyrers, G. Testa, and A. F. Stewart. 2000 DNA cloning by homologous recombination in Escherichia coli. Nat. Biotechnol. 18:1314-1317.
    • (2000) Nat. Biotechnol. , vol.18 , pp. 1314-1317
    • Zhang, Y.1    Muyrers, J.P.2    Testa, G.3    Stewart, A.F.4
  • 33
    • 3343001971 scopus 로고    scopus 로고
    • 2004. Kinetic studies of Thermobifida fusca Cel9A active site mutant enzymes
    • Zhou, W. L., D. C. Irwin, J. Escovar-Kousen, and D. B. Wilson. 2004 2004. Kinetic studies of Thermobifida fusca Cel9A active site mutant enzymes. Biochemistiy 43:9655-9663.
    • (2004) Biochemistiy , vol.43 , pp. 9655-9663
    • Zhou, W.L.1    Irwin, D.C.2    Escovar-Kousen, J.3    Wilson, D.B.4


* 이 정보는 Elsevier사의 SCOPUS DB에서 KISTI가 분석하여 추출한 것입니다.