Magnetic-activated cell sorting for sperm preparation reduces spermatozoa with apoptotic markers and improves the acrosome reaction in couples with unexplained infertility

Human Reproduction

Volumn 25, Issue 4, 2010, Pages 839-846

  Lee, Tsung Hsien ^a,b,c   Liu, Chung Hsien ^b   Shih, Yang Tse ^b   Tsao, Hui Mei ^d   Huang, Chun Chia ^d   Chen, Hsiu Hui ^d   Lee, Maw Sheng ^a,b,d  

^a CHUNG SHAN MEDICAL UNIVERSITY   (Taiwan)

^b CHUNG SHAN MEDICAL UNIVERSITY HOSPITAL   (Taiwan)

^c NATIONAL TAIWAN UNIVERSITY   (Taiwan)

^d Lee Women's Hospital   (Taiwan)

Author keywords

Acrosome reaction; Apoptotic markers; Infertility; Magnetic activated cell sorting; Semen analysis

Indexed keywords

DNA; FLUORESCENT DYE;

ACROSOME; ACROSOME REACTION; ADULT; APOPTOSIS; ARTICLE; CELL SELECTION; CONTROLLED STUDY; DENSITY GRADIENT CENTRIFUGATION; DNA FRAGMENTATION; FEMALE; FEMALE INFERTILITY; FERTILIZATION; FLOW CYTOMETRY; HUMAN; HUMAN CELL; INTRAUTERINE INSEMINATION; MAJOR CLINICAL STUDY; MALE; MALE INFERTILITY; MITOCHONDRIAL MEMBRANE POTENTIAL; SEMEN ANALYSIS; SPERMATOZOON; SPERMATOZOON COUNT; SPERMATOZOON MOTILITY; UNEXPLAINED INFERTILITY;

EID: 77949975818     PISSN: 02681161     EISSN: 14602350     Source Type: Journal    
DOI: 10.1093/humrep/deq009     Document Type: Article

References (33)

1. Arslan M, Morshedi M, Arslan EO, Taylor S, Kanik A, Duran HE, Oehninger S. Predictive value of the hemizona assay for pregnancy outcome in patients undergoing controlled ovarian hyperstimulation with intrauterine insemination. Fertil Steril 2006;85:1697-1707.
2. Aziz N, Agarwal A. Evaluation of sperm damage: beyond the World Health Organization criteria. Fertil Steril 2008;90:484-485.
3. Barroso G, Taylor S, Morshedi M, Manzur F, Gavino F, Oehninger S. Mitochondrial membrane potential integrity and plasma membrane translocation of phosphatidylserine as early apoptotic markers: a comparison of two different sperm subpopulations. Fertil Steril 2006; 85:149-154.
4. Crosignani PG, Collins J, Cooke ID, Diczfalusy E, Rubin B. Recommendations of the ESHRE workshop on 'Unexplained Infertility'. Anacapri, August 28-9, 1992. Hum Reprod 1993;8:977-980.
5. de Vantery AC, Lucas H, Chardonnens D, de Agostini A. Removal of spermatozoa with externalized phosphatidylserine from sperm preparation in human assisted medical procreation: effects on viability, motility and mitochondrial membrane potential. Reprod Biol Endocrinol 2009;7:1.
6. Dickey RP, Pyrzak R, Lu PY, Taylor SN, Rye PH. Comparison of the sperm quality necessary for successful intrauterine insemination with World Health Organization threshold values for normal sperm. Fertil Steril 1999;71:684-689.
7. Duran EH, Morshedi M, Taylor S, Oehninger S. Sperm DNA quality predicts intrauterine insemination outcome: a prospective cohort study. Hum Reprod 2002;17:3122-3128.
8. ESHRE Andrology Special Interest Group. Guidelines on the application of CASA technology in the analysis of spermatozoa. Hum Reprod 1998; 13:142-145.
9. Franken DR, Kruger TF, Oehninger S, Coddington CC, Lombard C, Smith K, Hodgen GD. The ability of the hemizona assay to predict human fertilization in different and consecutive in-vitro fertilization cycles. Hum Reprod 1993;8:1240-1244.
10. Gadella BM, Harrison RA. Capacitation induces cyclic adenosine 3,5-monophosphate-dependent, but apoptosis-unrelated, exposure of aminophospholipids at the apical head plasma membrane of boar sperm cells. Biol Reprod 2002;67:340-350.
11. Gandini L, Lombardo F, Paoli D, Caponecchia L, Familiari G, Verlengia C, Dondero F, Lenzi A. Study of apoptotic DNA fragmentation in human spermatozoa. Hum Reprod 2000;15:830-839.
12. Gearon CM, Mortimer D, Chapman MG, Forman RG. Artificial induction of the acrosome reaction in human spermatozoa. Hum Reprod 1994; 9:77-82.
13. Gleicher N, Barad D. Unexplained infertility: does it really exist? Hum Reprod 2006;21:1951-1955.
14. Grunewald S, Reinhardt M, Blumenauer V, Said TM, Agarwal A, Abu HF, Glander HJ, Paasch U. Increased sperm chromatin decondensation in selected nonapoptotic spermatozoa of patients with male infertility. Fertil Steril 2008;92:572-577.
15. Guzick DS, Factor-Litvak P, Steinkampf MP, Hill JA, Mastroianni L, Buster JE, Nakajima ST et al. Efficacy of superovulation and intrauterine insemination in the treatment of infertility. National Cooperative Reproductive Medicine Network. N Engl J Med 1999; 340:177-183.
16. Henkel RR, Schill WB. Sperm preparation for ART. Reprod Biol Endocrinol 2003;1:108.
17. Henkel R, Hajimohammad M, Stalf T, Hoogendijk C, Mehnert C, Menkveld R, Gips H, Schill WB, Kruger TF. Influence of deoxyribonucleic acid damage on fertilization and pregnancy. Fertil Steril 2004;81:965-972.
18. Huang CC, Lin DP, Tsao HM, Cheng TC, Liu CH, Lee MS. Sperm DNA fragmentation negatively correlates with velocity and fertilization rates but might not affect pregnancy rates. Fertil Steril 2005;84:130-140.
19. Kruger TF, Menkveld R, Stander FS, Lombard CJ, Van der Merwe JP, van Zyl JA, Smith K. Sperm morphologic features as a prognostic factor in in vitro fertilization. Fertil Steril 1986;46:1118-1123.
20. Larsen L, Scheike T, Jensen TK, Bonde JP, Ernst E, Hjollund NH, Zhou Y, Skakkebaek NE, Giwercman A. Computer-assisted semen analysis parameters as predictors for fertility of men from the general population. The Danish First Pregnancy Planner Study Team. Hum Reprod 2000;15:1562-1567.
21. Lee TH, Liu CH, Huang CC, Chen HH, Chen SU, Lee MS. The association between polypronucleate zygote formation with certain motion characteristics of sperm and IVF outcome. J Assist Reprod Genet 2008; 25:35-41.
22. Liu DY, Baker HW. Disordered acrosome reaction of spermatozoa bound to the zona pellucida: a newly discovered sperm defect causing infertility with reduced sperm-zona pellucida penetration and reduced fertilization in vitro. Hum Reprod 1994;9:1694-1700.
23. Liu DY, Baker HW. Disordered zona pellucida-induced acrosome reaction and failure of in vitro fertilization in patients with unexplained infertility. Fertil Steril 2003;79:74-80.
24. Liu DY, Baker HW. Human sperm bound to the zona pellucida have normal nuclear chromatin as assessed by acridine orange fluorescence. Hum Reprod 2007;22:1597-1602.
25. Liu DY, Clarke GN, Martic M, Garrett C, Baker HW. Frequency of disordered zona pellucida (ZP)-induced acrosome reaction in infertile men with normal semen analysis and normal spermatozoa-ZP binding. Hum Reprod 2001;16:1185-1190.
26. Oehninger S, Coddington CC, Scott R, Franken DA, Burkman LJ, Acosta AA, Hodgen GD. Hemizona assay: assessment of sperm dysfunction and prediction of in vitro fertilization outcome. Fertil Steril 1989;51:665-670.
27. Oehninger S, Franken DR, Sayed E, Barroso G, Kolm P. Sperm function assays and their predictive value for fertilization outcome in IVF therapy: a meta-analysis. Hum Reprod Update 2000;6:160-168.
28. Oosterhuis GJ, Mulder AB, Kalsbeek-Batenburg E, Lambalk CB, Schoemaker J, Vermes I. Measuring apoptosis in human spermatozoa: a biological assay for semen quality? Fertil Steril 2000;74:245-250.
29. Paasch U, Grunewald S, Agarwal A, Glandera HJ. Activation pattern of caspases in human spermatozoa. Fertil Steril 2004;81(Suppl. 1): 802-809.
30. Said TM, Grunewald S, Paasch U, Glander HJ, Baumann T, Kriegel C, Li L, Agarwal A. Advantage of combining magnetic cell separation with sperm preparation techniques. Reprod Biomed Online 2005;10:740-746.
31. Shen HM, Dai J, Chia SE, Lim A, Ong CN. Detection of apoptotic alterations in sperm in subfertile patients and their correlations with sperm quality. Hum Reprod 2002;17:1266-1273.
32. Siristatidis C, Bhattacharya S. Unexplained infertility: does it really exist? Does it matter? Hum Reprod 2007;22:2084-2087
33. WHO. WHO Laboratory Manual for the Examination of Human Semen and Semen-Cervical Mucus Interactions. Cambridge: Cambridge University Press, 1999.