DNA-PKcs regulates a single-stranded DNA endonuclease activity of Artemis

DNA Repair

Volumn 9, Issue 4, 2010, Pages 429-437

  Gu, Jiafeng ^a,b   Li, Sicong ^a,b   Zhang, Xiaoshan ^c   Wang, Ling Chi ^a   Niewolik, Doris ^d,e   Schwarz, Klaus ^d,e   Legerski, Randy J ^c   Zandi, Ebrahim ^a   Lieber, Michael R ^a,b  

^a UNIVERSITY OF SOUTHERN CALIFORNIA   (United States)

^b Section on Molecular and Computational Biology   (United States)

^c UNIVERSITY OF TEXAS MD ANDERSON CANCER CENTER   (United States)

^d German Red Cross Blood Transfusion Service Baden Württemberg Hessen and University Hospital Ulm   (Germany)

^e UNIVERSITY OF ULM   (Germany)

Author keywords

Double strand DNA break repair; NHEJ; Nonhomologous DNA end joining; Single stranded DNA endonuclease; V(D)J recombination

Indexed keywords

DEOXYRIBONUCLEASE; DIVALENT CATION; DNA; DOUBLE STRANDED DNA; ENDONUCLEASE; ENZYME ANTIBODY; NUCLEASE; PROTEIN ARTEMIS; PROTEIN KINASE C; RECOMBINANT ENZYME; SINGLE STRANDED DNA; UNCLASSIFIED DRUG;

ARTICLE; CONTROLLED STUDY; DNA RECOMBINATION; ENZYME ACTIVITY; ENZYME DEGRADATION; ENZYME REGULATION; ENZYME SUBSTRATE; GEL FILTRATION; PRIORITY JOURNAL; PROTEIN FUNCTION; PROTEIN PROCESSING; PROTEIN PURIFICATION; REGULATORY MECHANISM;

ANIMALS; CALCIUM-BINDING PROTEINS; CELL LINE; DEOXYRIBONUCLEASE I; DNA LIGASES; DNA REPAIR; HUMANS; NUCLEAR PROTEINS;

EID: 77949658435     PISSN: 15687864     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.dnarep.2010.01.001     Document Type: Article

References (22)

1. Lieber M.R. The mechanism of human nonhomologous DNA end joining. J. Biol. Chem. 283 (2008) 1-5
2. Ma Y., Pannicke U., Schwarz K., and Lieber M.R. Hairpin opening and overhang processing by an Artemis:DNA-PKcs complex in V(D)J recombination and in nonhomologous end joining. Cell 108 (2002) 781-794
3. Ma Y., Schwarz K., and Lieber M.R. The Artemis:DNA-PKcs endonuclease can cleave gaps, flaps, and loops. DNA Repair 4 (2005) 845-851
4. Anderson C.W., and Carter T.H. The DNA-activated protein kinase-DNA-PK. In: Jessberger R., and Lieber M.R. (Eds). Molecular Analysis of DNA Rearrangements in the Immune System (1996), Springer-Verlag, Heidelberg 91-112
5. Meek K., Dang V., and Lees-Miller S.P. DNA-PK: the means to justify the ends?. Adv. Immunol. 99 (2008) 33-58
6. Meek K., Gupta S., Ramsden D.A., and Lees-Miller S.P. The DNA-dependent protein kinase: the director at the end. Immunol. Rev. 200 (2004) 132-141
7. Ma Y., Pannicke U., Lu H., Niewolik D., Schwarz K., and Lieber M.R. The DNA-PKcs phosphorylation sites of human artemis. J. Biol. Chem. 280 (2005) 33839-33846
8. Niewolik D., Pannicke U., Lu H., Ma Y., Wang L.C., Kulesza P., Zandi E., Lieber M.R., and Schwarz K. DNA-PKcs dependence of artemis endonucleolytic activity: differences between hairpins and 5′ or 3′ overhangs. J. Biol. Chem. 281 (2006) 33900-33909
9. Yannone S.M., Khan I.S., Zhou R.Z., Zhou T., Valerie K., and Povirk L.F. Coordinate 5′ and 3′ endonucleolytic trimming of terminally blocked blunt DNA double-strand break ends by Artemis nuclease and DNA-dependent protein kinase. Nucleic Acids Res. 36 (2008) 3354-3365
10. Huang Y., Giblin W., Kubec M., Westfield G., St Charles J., Chadde L., Kraftson S., and Sekiguchi J. Impact of a hypomorphic Artemis disease allele on lymphocyte development, DNA end processing, and genome stability. J. Exp. Med. 206 (2009) 893-908
11. Lu H., Schwarz K., and Lieber M.R. Extent to which hairpin opening by the Artemis:DNA-PKcs complex can contribute to junctional diversity in V(D)J recombination. Nucleic Acids Res. (2007)
12. Howard F.B., Chen C., Ross P.D., and Miles H.T. Hairpin formation in the self-complementary dodecamer d-GGTACGCGTACC and derivatives containind GA and IA mispairs. Biochemistry 30 (1991) 779-782
13. Blommers M.J., Walters J.A., Haasnoot C.A., Aelen J.M., Marel G.v., Boom J.v., and Hilbers C.W. Effects of base sequence on the loop folding in DNA hairpins. Biochemistry 28 (1989) 7491-7498
14. Raghunathan G., Jernigan R.L., Miles H.T., and Sasisekharan V. Conformational feasibility of a hairpin with two purines in the loop. 5′-d-GGTACIAGTACC-3′. Biochemistry 30 (1991) 782-788
15. Zhang X., Succi J., Feng Z., Prithivirajsingh S., Story M., and Legerski R.J. Artemis is a phosphorylation target of ATM and ATR and is involved in the G2/M DNA damage checkpoint response. Mol. Cell. Biol. 24 (2004) 9207-9220
16. Goodarzi A.A., Yu Y., Riballo E., Douglas P., Walker S.A., Ye R., Harer C., Marchetti C., Morrice N., Jeggo P.A., and Lees-Miller S.P. DNA-PK autophosphorylation facilitates Artemis endonuclease activity. EMBO J. 25 (2006) 3880-3889
17. Povirk L.F., Zhou T., Zhou R., Cowan M.J., and Yannone S.M. Processing of 3′-phosphoglycolate-terminated DNA double strand breaks by Artemis nuclease. J. Biol. Chem. 282 (2007) 3547-3558
18. Pannicke U., Ma Y., Lieber M.R., and Schwarz K. Functional and biochemical dissection of the structure-specific nuclease Artemis. EMBO J. 23 (2004) 1987-1997
19. Kim C., Snyder R.O., and Wold M.S. Binding properties of replication protein A from human and yeast cells. Mol. Cell. Biol. 12 (1992) 3050-3059
20. Gu J., Lu H., Tsai A.G., Schwarz K., and Lieber M.R. Single-stranded DNA ligation and XLF-stimulated incompatible DNA end ligation by the XRCC4-DNA ligase IV complex: influence of terminal DNA sequence. Nucleic Acids Res. 35 (2007) 5755-5762
21. Gu J., Lu H., Tippin B., Shimazaki N., Goodman M.F., and Lieber M.R. XRCC4:DNA ligase IV can ligate incompatible DNA ends and can ligate across gaps. EMBO J. 26 (2007) 1010-1023
22. Lu H., Shimazaki N., Raval P., Gu J., Watanabe G., Schwarz K., Swanson P., and Lieber M.R. A biochemically defined system for coding joint formation in human V(D)J recombination. Mol. Cell 31 (2008) 485-497