TimeSTAMP tagging of newly synthesized proteins

Current Protocols in Protein Science

Volumn , Issue SUPPL. 59, 2010, Pages

  Lin, Michael Z ^a   Tsien, Roger Y ^b  

^a STANFORD UNIVERSITY   (United States)

^b HOWARD HUGHES MEDICAL INSTITUTE   (United States)

Author keywords

Conditional labeling; Protease; Protein synthesis

Indexed keywords

CILUPREVIR; CIS ACTING ELEMENT; EPITOPE; HYBRID PROTEIN; PROTEINASE; PROTEINASE INHIBITOR; RNA;

CELL STRUCTURE; CELLULAR DISTRIBUTION; CYTOLOGY; ENZYME ACTIVITY; ENZYME INHIBITION; GENETIC TRANSFECTION; HEPATITIS C VIRUS; IMMUNOBLOTTING; IMMUNOCYTOCHEMISTRY; PRIORITY JOURNAL; PROTEIN ANALYSIS; PROTEIN EXPRESSION; PROTEIN SYNTHESIS; QUANTITATIVE ANALYSIS; REVIEW; RNA TRANSLATION; AMINO TERMINAL SEQUENCE; ARTICLE; CARBOXY TERMINAL SEQUENCE; CHEMICAL LABELING; CLINICAL PROTOCOL; CROSS REACTION; EXPERIMENTAL STUDY; FUSION GENE; GENE CASSETTE; MOLECULAR CLONING; POLYACRYLAMIDE GEL ELECTROPHORESIS; PROTEIN LOCALIZATION;

ANIMALS; IMMUNOBLOTTING; IMMUNOHISTOCHEMISTRY; PROTEIN BIOSYNTHESIS; STAINING AND LABELING;

EID: 77949383394     PISSN: 19343655     EISSN: 19343663     Source Type: Journal    
DOI: 10.1002/0471140864.ps2605s59     Document Type: Review

References (13)

1. Ausubel, F.M., Brent, R., Kingston, R.E., Moore, D.D., Seidman, J.G., Smith, J.A., and, Struhl, K., (eds). 2008. Current Protocols in Molecular Biology. John Wiley & Sons. New York.
2. Bartenschlager, R., Lohmann, V., Wilkinson, T., and, Koch, J.O., 1995. Complex formation between the NS3 serine-type proteinase of the hepatitis C virus and NS4A and its importance for polyprotein maturation. J. Virol. 69: 7519 - 7528.
3. Dieterich, D.C., Lee, J.J., Link, A.J., Graumann, J., Tirrell, D.A., and, Schuman, E.M., 2007. Labeling, detection and identification of newly synthesized proteomes with bioorthogonal non-canonical amino-acid tagging. Nat. Protoc. 2: 532 - 540.
4. Flores, M.V., Strawbridge, J., Ciaramella, G., and, Corbau, R., 2009. HCV-NS3 inhibitors: Determination of their kinetic parameters and mechanism. Biochim. Biophys. Acta. 1794: 1441 - 1448.
5. Ingallinella, P., Altamura, S., Bianchi, E., Taliani, M., Ingenito, R., Cortese, R., De Francesco, R., Steinkuhler, C., and, Pessi, A., 1998. Potent peptide inhibitors of human hepatitis C virus NS3 protease are obtained by optimizing the cleavage products. Biochemistry 37: 8906 - 8914.
6. Ingallinella, P., Bianchi, E., Ingenito, R., Koch, U., Steinkuhler, C., Altamura, S., and, Pessi, A., 2000. Optimization of the P′-region of peptide inhibitors of hepatitis C virus NS3/4A protease. Biochemistry 39: 12898 - 12906.
7. Kourtis, N., and, Tavernarakis, N., 2009. Cell-specific monitoring of protein synthesis in vivo. PLoS One 4: e4547.
8. Landro, J.A., Raybuck, S.A., Luong, Y.P., OMalley, E.T., Harbeson, S.L., Morgenstern, K.A., Rao, G., and, Livingston, D.J., 1997. Mechanistic role of an NS4A peptide cofactor with the truncated NS3 protease of hepatitis C virus: Elucidation of the NS4A stimulatory effect via kinetic analysis and inhibitor mapping. Biochemistry 36: 9340 - 9348.
9. Lin, M.Z., Glenn, J.S., and, Tsien, R.Y., 2008. A drug-controllable tag for visualizing newly synthesized proteins in cells and whole animals. Proc. Natl. Acad. Sci. U.S.A. 105: 7744 - 7749.
10. Mili, S., and, Macara, I.G., 2009. RNA localization and polarity: from A(PC) to Z(BP). Trends Cell Biol. 19: 156 - 164.
11. Rock, K.L., York, I.A., and, Goldberg, A.L., 2004. Post-proteasomal antigen processing for major histocompatibility complex class I presentation. Nat. Immunol. 5: 670 - 677.
12. Tong, X., Chase, R., Skelton, A., Chen, T., Wright-Minogue, J., and, Malcolm, B.A., 2006. Identification and analysis of fitness of resistance mutations against the HCV protease inhibitor SCH 503034. Antiviral Res. 70: 28 - 38.
13. Lin et al., 2008. See above. Describes design and use of TimeSTAMP to track newly synthesized proteins of interest by immunoblotting and immunocytochemistry.