Extraction and partial purification of β-galactosidase from grape berry skin (Vitis vinifera L.) with triton X-114

American Journal of Enology and Viticulture

Volumn 61, Issue 1, 2010, Pages 135-139

  Pérez Gilabert, Manuela ^a   Téllez, Edgar ^a   García Carmona, Francisco ^a  

^a UNIVERSITY OF MURCIA   (Spain)

Author keywords

galactosidase; Extraction; Grape berry skin; Monastrell; Phase partitioning; Triton X 114

Indexed keywords

VITACEAE; VITIS VINIFERA;

EID: 77949303595     PISSN: 00029254     EISSN: None     Source Type: Journal    
DOI: None     Document Type: Article

References (26)

1. Alcântara, P.H.N., L. Martim, C.O. Silva, S.M.C. Dietrich, and M.S. Buckeridge. 2006. Purification of a β-galactosidase from cotyledons of Hymenaea courbaril L. (Leguminosae). Enzyme properties and biological function. Plant Physiol. Biochem. 44:619-627.
2. Balasubramaniam, S., H.C. Lee, H. Lazan, R. Toman, and Z.M. Ali. 2005. Purification and properties of a β-galactosidase from carambola fruit with significant activity towards cell wall polysaccharides. Phytochemistry 66:153-163.
3. Barnavon, L., T. Doco, N. Terrier, A. Ageorges, C. Romieu, and P. Pellerin. 2000. Analysis of cell wall neutral sugar composition, β-galactosidase activity and a related cDNA clone throughout the development of Vitis vinifera grape berries. Plant Physiol. Biochem. 38:289-300.
4. Brown, R.E., K.L. Jarvis, and K.J. Hyland. 1989. Protein measurement using bicinchoninic acid: Elimination of interfering substances. Anal. Biochem. 180:136-139.
5. Chantarangsee, M., W. Tanthanuch, T. Fujimura, S.C. Fry, and J.K. Cairns. 2007. Molecular characterization of β-galactosidase from germinating rice (Oryza sativa). Plant Sci. 173:118-134.
6. Dick, A., A. Opoku-Gyamfua, and A.C. De Marco. 1990. Glycosidases of apple fruit: A multi-functional β-galactosidase. Physiol. Plant. 80:250-256.
7. Gantulga, D., Y. Turan, D.R. Bevan, and A. Esen. 2008. The Arabidopsis At1g45130 and At3g52840 genes encode β-galactosidases with activity toward cell wall polysaccharides. Phytochemistry 69:1661-1670.
8. Gerardi, C., F. Blando, A. Santino, and G. Zacheo. 2001. Purification and characterisation of a β-glucosidase abundantly expressed in ripe sweet cherry (Prunus avium L.) fruit. Plant Sci. 160:795-805.
9. Golden, K.D., M.A. John, and E.A. Kean. 1993. β-Galactosidase from Coffea arabica and its role in fruit ripening. Phytochemistry 34:355-360.
10. Henrissat, B.A 1991. Classification of glycosyl hydrolases based on amino acid sequence similarities. Biochem. J. 280:309-316.
11. Konno, H., S. Nakashima, T. Nakato, and K. Katoh. 2002. Pectin-bound β-galactosidase present in cell walls of carrot cells under the different calcium status. Physiol. Plant. 114:213-222.
12. Kuby, S.A., and H.A. Lardy. 1953. Purification and kinetics of β-galactosidase from E. coli strain K2. J. Am. Chem. Soc. 75:890-896.
13. Li, S.C., J.W. Han, K.C. Chen, and C.S. Chen. 2001. Purification and characterization of isoforms of β-galactosidases in mung bean seedlings. Phytochemistry 57:349-359.
14. Marquart, D.W. 1963. An algorithm for least-squares estimation of non-linear parameters. J. Soc. Ind. Appl. Math. 11:431-441.
15. Nunan, K.J., C. Davies, S.P. Robinson, and G.B. Fincher. 2001. Expression patterns of cell wall-modifying enzymes during grape berry development. Planta 214:257-264.
16. Ortega-Regules, A., J.M. Ros-García, A.B. Bautista-Ortín, J.M. López-Roca, and E. Gómez-Plaza. 2008. Differences in morphology and composition of skin and pulp cell walls from grapes (Vitis vinifera L.): Technological implications. Eur. Food Res. Tech. 227:223-231.
17. Pérez-Gilabert, M., J.M. López-Nicolás, and F. García-Carmona. 2001a. Purification of a novel lipoxygenase from eggplant (Solanum melongena) fruit chloroplasts. Physiol. Plant. 111:276-282.
18. Pérez-Gilabert, M., A. Morte, M. Honrubia, and F. García-Carmona. 2001b. Partial purification, characterization, and histochemical localization of fully latent desert truffle (Terfezia claveryi Chatin) polyphenol oxidase. J. Agric. Food Chem. 49:1922-1927.
19. Pérez-Gilabert, M., I. Sánchez-Felipe, and F. García-Carmona. 2005. Purification and partial charaterization of lipoxygenase from desert truffle (Terfezia claveryi Chatin) ascocarps. J. Agric. Food Chem. 53:3666-3671.
20. Pérez-Gilabert, M., E. Téllez, and F. García-Carmona. 2009. Triton X-114-aided extraction and partial characterization of β-galactosidase from grape berry pulp (Vitis vinifera L.). Am. J. Enol. Vitic. 60:368-372.
21. Prasanna, V., T.N. Prabha, and R.N. Tharanathan. 2005. Multiple forms of β-galactosidase from mango (Mangifera indica L. Alphonso) fruit pulp. J. Sci. Food Agric. 85:797-803.
22. Pressey, R. 1983. β-Galactosidase in ripening tomatoes. Plant Physiol. 71:132-135.
23. Sánchez-Ferrer, A., M. Pérez-Gilabert, E. Núñez, R. Bru, and F. García-Carmona. 1994. Triton X-114 phase partitioning in plant protein purification. J. Chromatogr., A 668:75-83.
24. Simos, G., and J.G. Georgatsos. 1988. Lactose-hydrolyzing β-glycosidases of barley meal. Biochim. Biophys. Acta 967:17-24.
25. Singleton, V.L., and J.A. Rossi, Jr. 1965. Colorimetry of total phenolics with phosphomolybdic-phosphotungstic acid reagents. Am. J. Enol. Vitic. 16:144-158.
26. Smith, P.K., R.I. Krohn, G.T. Hermanson, A.K. Mallia, F.H. Gartner, M.D. Provenzano, E.K. Fujimoto, N.M. Goeke, B.J. Olson, and D.C. Klenk. 1985. Measurement of protein using bicinchoninic acid. Anal. Biochem. 150:76-85.