Triton X-114-aided extraction and partial characterization of β-galactosidase from grape berry pulp (Vitis vinifera L.)

American Journal of Enology and Viticulture

Volumn 60, Issue 3, 2009, Pages 368-372

  Pérez Gilabert, Manuela ^a   Téllez, Edgar ^a   García Carmona, Francisco ^a  

^a UNIVERSITY OF MURCIA   (Spain)

Author keywords

galactosidase; Phase partitioning; Pulp; Triton X 114

Indexed keywords

VITACEAE; VITIS VINIFERA;

EID: 70349499665     PISSN: 00029254     EISSN: None     Source Type: Journal    
DOI: None     Document Type: Article

References (29)

1. Alcântara, P.H.N., L. Martim, C.O. Silva, S.M.C. Dietrich, and M.S. Buckeridge. 2006. Purification of a β-galactosidase from cotyledons of Hymenaea courbaril L. (Leguminosae). Enzyme properties and biological function. Plant Physiol. Biochem. 44:619-627.
2. Balasubramaniam, S., H.C. Lee, H. Lazan, R. Toman, and Z.M. Ali. 2005. Purification and properties of a β-galactosidase from carambola fruit with significant activity towards cell wall polysaccharides. Phytochemistry 66:153-163.
3. Barnavon, L., T. Doco, N. Terrier, A. Ageorges, C. Romieu, and P. Pellerin. 2000. Analysis of cell wall neutral sugar composition, β-galactosidase activity and a related cDNA clone throughout the development of Vitis vinifera grape berries. Plant Physiol. Biochem. 38:289-300.
4. Batisse, C., M. Buret, and P.J. Coulomb. 1996. Biochemical dif ferences in cell wall of cherry fruit between soft and crisp fruit. J. Agric. Food Chem. 44:453-457.
5. Brown, R.E., K.L. Janvis, and K.J. Hyland. 1989. Protein measurement using bicinchoninic acid elimination of interfering substances. Anal. Biochem. 180:136-139.
6. Chantarangsee, M., W. Tanthanuch, T. Fujimura, S.C. Fry, and J.K. Cairns. 2007. Molecular characterization of β-galactosidase from germinating rice (Oryza sativa). Plant Sci. 173:118-134.
7. Dick, A., A. Opoku-Gyamfua, and A.C. DeMarco. 1990. Glycosi dases of apple fruit: A multi-functional β-galactosidase. Physiol. Plant. 80:250-256.
8. Gantulga, D., Y. Turan, D.R. Bevan, and A. Esen. 2008. The Arabi dopsis At1g45130 and At3g52840 genes encode β-galactosidases with activity toward cell wall polysaccharides. Phytochemistry 69:1661-1670.
9. Gil-Muñoz, R., A. Hernández-Jiménez, J.M. López-Roca, A. Martínez-Cutillas, and E. Gómez-Plaza. 2007. Efecto de difer entes enzimas de maceración sobre el color de vinos de Syrah, Monastrell y Cabernet Sauvignon. Poster presentation. 30th World Congress of Vine and Wine, Budapest.
10. Golden, K.D., M.A. John, and E.A. Kean. 1993. β-Galactosidase from Coffea arabica and its role in fruit ripening. Phytochemistry 34:355-360.
11. Kovács, E., P. Merész, Z. Kristóf, and E. Németh-Szerdahelyi. 2008. Ripening and microstructure of apricot (Prunus armeniaca L.) Acta Alimen. 37:23-39.
12. Konno, H., S. Nakashima, T. Nakato, and K. Katoh. 2002. Pectin bound β-galactosidase present in cell walls of carrot cells under the different calcium status. Physiol. Plant. 114:213-222.
13. Li, S.C., J.W. Han, K.C. Chen, and C.S. Chen. 2001. Purification and characterization of isoforms of β-galactosidases in mung bean seedlings. Phytochemistry 57:349-359.
14. Marquardt, D.W. 1963. An algorithm for least-squares estimation of non-linear parameters. J. Soc. Ind. Appl. Math. 11:431-441.
15. Nunan, K.J., I.M. Sims, A. Bacic, S.P. Robinson, and G.B. Fincher. 1998. Changes in cell wall composition during ripening of grape berries. Plant Physiol. 118:783-792.
16. Nunan, K.J., C. Davies, S.P. Robinson, and G.B. Fincher. 2001. Expression patterns of cell wall-modifying enzymes during grape berry development. Planta 214:257-264.
17. Ortega-Regules, A., J.M. Ros-García, A. B. Bautista-Ortín, J.M. López-Roca, and E. Gómez-Plaza. 2008. Differences in morphol ogy and composition of skin and pulp cell walls from grapes (Vitis vinifera L.): Technological implications. Eur. Food Res. Technol. 227:223-231.
18. Pérez-Gilabert, M., J.M. López-Nicolás, and F. García-Carmona. 2001a. Purification of a novel lipoxygenase from eggplant (Solanum melongena) fruit chloroplasts. Physiol. Plant. 111:276-282.
19. Pérez-Gilabert, M., A. Morte, M. Honrubia, and F. García-Carmona. 2001b. Partial purification, characterization, and histochemical localization of fully latent desert truffle (Terfezia claveryi Chatin) polyphenol oxidase. J. Agric. Food Chem. 49:1922-1927.
20. Pérez-Gilabert, M., I. Sánchez-Felipe, and F. García-Carmona. 2005. Purification and partial charaterization of lipoxygenase from desert truffle (Terfezia claveryi Chatin) ascocarps. J. Agric. Food Chem. 53:3666-3671.
21. Pérez-Magariño, S., and M.L.González-San José. 2005. Effect of ripening of grapes on the low molecular weight phenolic com pounds of red wines. Eur. Food Res. Technol. 220:597-606.
22. Prasanna, V., T.N. Prabha, and R.N. Tharanathan. 2005. Multiple forms of β-galactosidase from mango (Mangifera indica L. Al phonso) fruit pulp. J. Sci. Food Agric. 85:797-803.
23. Pressey, R. 1983. β-Galactosidase in ripening tomatoes. Plant Physiol. 71:132-135.
24. Romero-Cascales, I., J.I. Fernández-Fernández, J.M. Ros-García, J.M. López-Roca, and E. Gómez-Plaza. 2008. Characterisation of the main enzymatic activities present in six commercial macerating enzymes and their effects on extracting colour during winemaking of Monastrell grapes. Int. J. Food Sci. Technol. 43:1295-1305.
25. Sánchez-Ferrer, A., M. Pérez-Gilabert, E. Núñez, R. Bru, and F. García-Carmona. 1994. Triton X-114 phase partitioning in plant protein purification. J. Chromatogr., A 668:75-83.
26. Simos, G., and J.G. Georgatsos. 1988. Lactose-hydrolyzing β-glycosidases of barley meal. Biochim. Biophis. Acta 967:17 24.
27. Singleton, V.L., and J.A. Rossi Jr. 1965. Colorimetry of total phenolics with phosphomolybdic-phosphotungstic acid reagents. Am. J. Enol. Vitic. 16:144-158.
28. Smith, P.K., et al. 1985. Measurement of protein using bicinchoninic acid. Anal. Biochem. 150:76-85.
29. Smith, D.L., J.A. Abbot, and K.C. Gross. 2002. Down-regulation of tomato β-galactosidase 4 results in decreased fruit softening. Plant Physiol. 129:1755-1762.