Use of bicistronic vectors in combination with flow cytometry to screen for effective small interfering RNA target sequences

Biochemical and Biophysical Research Communications

Volumn 393, Issue 3, 2010, Pages 498-503

  Kamio, Naoka ^a,b   Hirai, Hideyo ^a,b   Ashihara, Eishi ^a   Tenen, Daniel G ^c,d   Maekawa, Taira ^a   Imanishi, Jiro ^b  

^a KYOTO UNIVERSITY HOSPITAL   (Japan)

^b KYOTO PREFECTURAL UNIVERSITY OF MEDICINE   (Japan)

^c HARVARD STEM CELL INSTITUTE   (United States)

^d NATIONAL UNIVERSITY OF SINGAPORE   (Singapore)

Author keywords

Bicistronic vector; Flow cytometry; Green fluorescent protein; siRNA

Indexed keywords

GREEN FLUORESCENT PROTEIN; RED FLUORESCENT PROTEIN; SMALL INTERFERING RNA;

ARTICLE; EXPRESSION VECTOR; FLOW CYTOMETRY; GENE EXPRESSION; GENE SILENCING; GENE TARGETING; PRIORITY JOURNAL; QUANTITATIVE ANALYSIS; RNA SEQUENCE;

CELL LINE; FLOW CYTOMETRY; GENES, REPORTER; GENETIC VECTORS; GREEN FLUORESCENT PROTEINS; HUMANS; LUMINESCENT PROTEINS; RNA, SMALL INTERFERING; TRANSDUCTION, GENETIC;

EID: 77949264248     PISSN: 0006291X     EISSN: 10902104     Source Type: Journal    
DOI: 10.1016/j.bbrc.2010.02.033     Document Type: Article

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