Molecular cloning and characterization of gene encoding for cytoplasmic Hsc70 from Pennisetum glaucum may play a protective role against abiotic stresses

Molecular Genetics and Genomics

Volumn 283, Issue 3, 2010, Pages 243-254

  Reddy, Palakolanu Sudhakar ^a   Mallikarjuna, Garladinne ^a   Kaul, Tanushri ^a   Chakradhar, Thammineni ^a   Mishra, Rabi N ^a   Sopory, Sudhir K ^a   Reddy, Malireddy K ^a  

^a INTERNATIONAL CENTRE FOR GENETIC ENGINEERING AND BIOTECHNOLOGY ICGEB   (India)

Author keywords

CDNA library screening; Molecular chaperone; Recombinant protein; Thermotolerance; Transcript analysis

Indexed keywords

ADENOSINE TRIPHOSPHATASE; COMPLEMENTARY DNA; HEAT SHOCK PROTEIN 70; RESTRICTION ENDONUCLEASE;

ABIOTIC STRESS; ARTICLE; CHEMICAL STRUCTURE; CODING; CONTROLLED STUDY; ESCHERICHIA COLI; GENE; GENE DUPLICATION; GENE OVEREXPRESSION; GENETIC CODE; HEAT SHOCK COMPONENT 70 GENE; IN VITRO STUDY; INTRON; ISOLATION PROCEDURE; MOLECULAR CLONING; NONHUMAN; PEARL MILLET; PHYLOGENY; PRIORITY JOURNAL; PROMOTER REGION; PROTEIN PURIFICATION; THERMOSTABILITY; UPREGULATION;

AMINO ACID SEQUENCE; BASE SEQUENCE; CLONING, MOLECULAR; DNA PRIMERS; DNA, COMPLEMENTARY; DNA, PLANT; EXPRESSED SEQUENCE TAGS; HSC70 HEAT-SHOCK PROTEINS; MOLECULAR CHAPERONES; MOLECULAR SEQUENCE DATA; ORYZA SATIVA; PENNISETUM; PLANT PROTEINS; RECOMBINANT PROTEINS; TRANSCRIPTION, GENETIC;

PENNISETUM GLAUCUM;

EID: 77949263576     PISSN: 16174615     EISSN: None     Source Type: Journal    
DOI: 10.1007/s00438-010-0518-7     Document Type: Article

References (29)

1. F Aparicio CL Thomas C Lederer Y Niu DW Wang AJ Maule 2005 Virus induction of heat-shock protein 70 reflects a general response to protein accumulation in the plant cytosol Plant Physiol 138 529 536 10.1104/pp.104. 058958 1:CAS:528:DC%2BD2MXks12hs7k%3D 15805473 (Pubitemid 43138149)
2. WR Boorstein T Ziegelhoffer EA Craig 1994 Molecular evolution of the Hsp70 multigene family J Mol Evol 38 1 17 10.1007/BF00175490 1:CAS:528:DyaK2cXht1Knu74%3D 8151709
3. B Bukau AL Horwich 1998 The Hsp70 and Hsp60 chaperone machines Cell 92 351 366 10.1016/S0092-8674(00)80928-9 1:CAS:528:DyaK1cXhtFGisLk%3D 9476895 (Pubitemid 28093013)
4. P Chomczynski N Sacchi 1987 Single-step method of RNA isolation by acid guanidium thiocyanate-phenol-chloroform extraction Anal Biochem 162 156 159 10.1016/0003-2697(87)90021-2 1:CAS:528:DyaL2sXitFSns7Y%3D 2440339
5. KM Flaherty FC DeLuca DB McKay 1990 Three-dimensional structure of the ATPase fragment of a 70 K heat-shock cognate protein Nature 346 623 628 10.1038/346623a0 1:CAS:528:DyaK3cXlsVOqtbc%3D 2143562 (Pubitemid 20266393)
6. KM Flaherty FC DeLuca DB McKay 1994 Three-dimensional structure of ATP-ase fragment of a 70-kD heat-shock cognate protein Nature 346 623 628 10.1038/346623a0
7. J Frydman 2001 Folding of newly translated proteins in vivo: the role of molecular chaperones Annu Rev Biochem 70 603 647 10.1146/annurev.biochem.70.1. 603 1:CAS:528:DC%2BD3MXlsVehtLo%3D 11395418 (Pubitemid 32662220)
8. SC Gässler A Buchberger T Laufen MP Mayer H Schröder A Valencia B Bukau 1998 Mutations in the DnaK chaperone affecting interaction with the DnaJ co-chaperone Biochemistry 95 15229 15234
9. CL Guy QB Li 1998 The organization and evolution of the spinach stress 70 molecular chaperone gene family Plant Cell 10 539 556 10.1105/tpc.10.4.539 1:CAS:528:DyaK1cXivFCrsrY%3D 9548981 (Pubitemid 28542197)
10. FU Hartl 1996 Molecular chaperones in cellular protein folding Nature 381 571 580 10.1038/381571a0 1:CAS:528:DyaK28XjsFyltb8%3D 8637592 (Pubitemid 26177472)
11. He Z, Xie R, Wang Y, Zou H, Zhu J, Yu G (2008) Cloning and characterization of a heat-shock protein 70 gene, MsHsp70-1 in Medicago sativa. Acta Biochim Biophys Sin 40:209-216
12. JP Joshi 1987 An inspection of the domain between putative TATA box and translation start site in 79 plant genes Nucleic Acids Res 15 6643 6653 10.1093/nar/15.16.6643 1:CAS:528:DyaL2sXlsVyntL0%3D 3628002
13. JP Joshi 1987 Putative polyadenylation signals in nuclear genes of higher plants: a compilation and analysis Nucleic Acids Res 15 9627 9640 10.1093/nar/15.23.9627 1:CAS:528:DyaL1cXltVGgtQ%3D%3D 3697078
14. S Karlin L Brocchieri 1998 Heat-shock protein 70 family: multiple sequence comparisons, function, and evolution J Mol Evol 47 565 577 10.1007/PL00006413 1:CAS:528:DyaK1cXntFWhsbw%3D 9797407 (Pubitemid 28507477)
15. JG Kiang GC Tsokos 1998 Heat-shock protein 70 kDa: molecular biology, biochemistry and physiology Pharmacol Ther 80 183 201 10.1016/S0163-7258(98) 00028-X 1:CAS:528:DyaK1cXmslyrsrY%3D 9839771 (Pubitemid 28498227)
16. MA Lodhi GN Ye NF Weeden BI Reisch 1994 A simple and efficient method for DNA extraction from grapevine cultivars and Vitis species and Ampelopsis Plant Mol Biol Rep 12 6 13 10.1007/BF02668658 1:CAS:528:DyaK2cXltVensbg%3D
17. R Mahroof KY Zhu L Neven B Subramanyam J Bai 2005 Expression patterns of three heat-shock protein 70 genes among developmental stages of the red flour beetle, Tribolium castaneum (Coleoptera: Tenebrionidae) Comp Biochem Physiol A Mol Integr Physiol 141 247 256 10.1016/j.cbpb.2005.05.044 15979913 (Pubitemid 40965759)
18. RN Mishra PS Reddy S Nair G Markandeya AR Reddy SK Sopory MK Reddy 2007 Isolation and characterization of expressed sequence tags (ESTs) from subtracted cDNA libraries of Pennisetum glaucum seedlings Plant Mol Biol 64 713 732 10.1007/s11103-007-9193-4 1:CAS:528:DC%2BD2sXntlajsLY%3D 17558562 (Pubitemid 47036787)
19. EW Napolitano JS Pachter RK Liem 1987 Intracellular distribution of mammalian stress proteins. Effects of cytoskeletal-specific agents J Biol Chem 262 1493 1504 1:CAS:528:DyaL2sXhtVSit7w%3D 2879839
20. N Nikolaidis M Nei 2004 Concerted and nonconcerted evolution of the Hsp70 gene super family in two sibling species of nematodes Mol Biol Evol 21 498 505 10.1093/molbev/msh041 1:CAS:528:DC%2BD2cXis1entL0%3D 14694072 (Pubitemid 38339666)
21. MW Pfaffl GW Horgan L Dempfle 2002 Relative expression software tool (REST) for group-wise comparison and statistical analysis of relative expression results in real-time PCR Nucleic Acids Res 30 e36 10.1093/nar/30.9.e36 11972351
22. PS Reddy S Mahanty T Kaul S Nair SK Sopory MK Reddy 2008 A high-throughput genome walking method and its use for cloning unknown flanking sequences Anal Biochem 381 248 253 10.1016/j.ab.2008.07.012 1:CAS:528: DC%2BD1cXhtVOhs7zL 18674512
23. PS Reddy S Nair G Mallikarjuna T Kaul G Markandeya SK Sopory MK Reddy 2008 A high-throughput, low-cost method for the preparation of "sequencing-ready" phage DNA template Anal Biochem 376 258 261 10.1016/j.ab.2008.02.015 1:CAS:528:DC%2BD1cXkslejtr8%3D 18334224
24. T Renner ER Waters 2007 Comparative genomic analysis of the Hsp70 s from five diverse photosynthetic eukaryotes Cell Stress Chaperones 12 172 185 10.1379/CSC-230R1.1 1:CAS:528:DC%2BD2sXoslGntbY%3D 17688196 (Pubitemid 350224166)
25. A Sali TL Blundell 1993 Comparative protein modelling by satisfaction of spatial restraints J Mol Biol 234 779 815 10.1006/jmbi.1993.1626 1:CAS:528:DyaK2cXnt1ylug%3D%3D 8254673 (Pubitemid 24007801)
26. H Santacruz S Vriz N Angelier 1997 Molecular characterization of a heat-shock cognate cDNA of zebrafish, hsc70, and developmental expression of the corresponding transcripts Dev Genet 21 223 233 10.1002/(SICI)1520-6408(1997)21: 3<223::AID-DVG5>3.0.CO;2-9 1:CAS:528:DyaK2sXotVSms7s%3D 9397538 (Pubitemid 27514819)
27. P Santhoshkumar KK Sharma 2001 Analysis of alpha-crystallin chaperone function using restriction enzymes and citrate synthase Mol Vis 7 172 177 1:CAS:528:DC%2BD3MXntl2mtbs%3D 11483893
28. DY Sung E Vierling CL Guy 2001 Comprehensive expression profile analysis of the Arabidopsis Hsp70 gene family Plant Physiol 126 789 800 10.1104/pp.126.2.789 1:CAS:528:DC%2BD3MXks1Gms7Y%3D 11402207 (Pubitemid 32568814)
29. W Wang B Vinocur O Shoseyov A Altman 2004 Role of plant heat-shock proteins and molecular chaperones in the abiotic stress response Trends Plant Sci 9 244 252 10.1016/j.tplants.2004.03.006 1:CAS:528:DC%2BD2cXjvVemtbw%3D 15130550 (Pubitemid 38617223)