Preparation of the immobilized metal affinity membrane with high amount of metal ions and protein adsorption efficiencies

Process Biochemistry

Volumn 45, Issue 4, 2010, Pages 500-506

  Ke, Yi Miao ^a   Chen, Chih I ^a,b   Kao, Po Min ^a   Chen, Hua Bing ^a   Huang, Hung Chang ^c   Yao, Cheng Ju ^a   Liu, Yung Chuan ^a  

^a NATIONAL CHUNG HSING UNIVERSITY   (Taiwan)

^b HSIUPING INSTITUTE OF TECHNOLOGY   (Taiwan)

^c INSTITUTE OF NUCLEAR ENERGY RESEARCH   (Taiwan)

Author keywords

Enzymes purification; Immobilized metal affinity membrane; Optimization; Penicillin G acylase; Regenerated cellulose membrane

Indexed keywords

COPPER IONS; EPICHLOROHYDRIN; FLOWTHROUGH; IMMOBILIZED METAL AFFINITY MEMBRANES; MODEL PROTEINS; NAOH CONCENTRATION; OBJECTIVE FUNCTIONS; OPTIMAL REACTION CONDITION; PENICILLIN G ACYLASE; PROTEIN ADSORPTION; PROTEIN ADSORPTION CAPACITY; REGENERATED CELLULOSE; TRANSMEMBRANE PRESSURE DROP;

ADSORPTION; CATALYSTS; CELLULOSE; DIFFERENTIAL AMPLIFIERS; DRUG PRODUCTS; ENZYMES; METAL IONS; METAL REFINING; METALS; OPTIMIZATION; PRESSURE DROP; PROGRAMMED CONTROL SYSTEMS; PURIFICATION; TRACE ANALYSIS;

MEMBRANES;

ADSORPTION; AMPLIFIERS; CATALYSTS; CELLULOSE; IONS; MEMBRANES; METALS; OPTIMIZATION; PRESSURE GRADIENT; PROTEINS; PURIFICATION;

EID: 77649341325     PISSN: 13595113     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.procbio.2009.11.010     Document Type: Article

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