Dominance of highly divergent feline leukemia virus A progeny variants in a cat with recurrent viremia and fatal lymphoma

Retrovirology

Volumn 7, Issue , 2010, Pages

  Helfer Hungerbuehler, A Katrin ^a   Cattori, Valentino ^a   Boretti, Felicitas S ^a   Ossent, Pete ^a   Grest, Paula ^a   Reinacher, Manfred ^b   Henrich, Manfred ^b   Bauer, Eva ^a   Bauer Pham, Kim ^a   Niederer, Eva ^a   Holznagel, Edgar ^a   Lutz, Hans ^a   Hofmann Lehmann, Regina ^a  

^a UNIVERSITY OF ZURICH   (Switzerland)

^b JUSTUS LIEBIG UNIVERSITY   (Germany)

Author keywords

[No Author keywords available]

Indexed keywords

ENVELOPE PROTEIN; VIRUS ANTIGEN; VIRUS RNA; VIRUS ENVELOPE PROTEIN;

ANEMIA; ANIMAL EXPERIMENT; ANIMAL MODEL; ANIMAL TISSUE; ANTIGEN DETECTION; ARTICLE; B CELL LYMPHOMA; BLOOD ANALYSIS; CAT DISEASE; CD4+ T LYMPHOCYTE; CONTROLLED STUDY; DISEASE SEVERITY; FELINE LEUKEMIA VIRUS; FELINE LEUKEMIA VIRUS A; FEMALE; GENE SEQUENCE; LEUKOPENIA; LONG TERMINAL REPEAT; LYMPHOCYTE DEPLETION; LYMPHOMA; MUTAGENESIS; NONHUMAN; NUCLEOTIDE SEQUENCE; POINT MUTATION; PROGENY; RECURRENT INFECTION; RNA ANALYSIS; SALIVA ANALYSIS; VIRUS CELL INTERACTION; VIRUS LOAD; ANIMAL; ANIMAL DISEASE; BLOOD; CAT; CD4 LYMPHOCYTE COUNT; CLASSIFICATION; CLUSTER ANALYSIS; DNA SEQUENCE; FELINE IMMUNODEFICIENCY VIRUS; GENETIC POLYMORPHISM; GENETICS; ISOLATION AND PURIFICATION; PHYLOGENY; RECURRENT DISEASE; SALIVA; VIREMIA; VIROLOGY; VIRUS ACTIVATION;

FELINE IMMUNODEFICIENCY VIRUS; FELINE LEUKEMIA VIRUS;

ANIMALS; ANTIGENS, VIRAL; BLOOD; CATS; CD4 LYMPHOCYTE COUNT; CLUSTER ANALYSIS; FEMALE; IMMUNODEFICIENCY VIRUS, FELINE; LEUKEMIA VIRUS, FELINE; LYMPHOMA, B-CELL; PHYLOGENY; POINT MUTATION; POLYMORPHISM, GENETIC; RECURRENCE; RNA, VIRAL; SALIVA; SEQUENCE ANALYSIS, DNA; VIRAL ENVELOPE PROTEINS; VIRAL LOAD; VIREMIA; VIRUS ACTIVATION;

EID: 77649288491     PISSN: None     EISSN: 17424690     Source Type: Journal    
DOI: 10.1186/1742-4690-7-14     Document Type: Article

References (83)

1. Jarrett WF, Crawford EM, Martin WM, Davie F. A virus-like particle associated with leukaemia (lymphosarcoma). Nature 1964, 202:567-568. 10.1038/202567a0, 14195054.
2. Pedersen NC, Ho EW, Brown ML, Yamamoto JK. Isolation of a T-lymphotropic virus from domestic cats with an immunodeficiency-like syndrome. Science 1987, 235:790-793. 10.1126/science.3643650, 3643650.
3. Yamamoto JK, Sparger E, Ho EW, Andersen PR, OConner TP, Mandell CP, Lowenstinde L, Munn R, Pedersen NC. Pathogenesis of experimentally induced feline immunodeficiency virus infection in cats. Am J Vet Res 1988, 49:1246-1258.
4. Hoover EA, Mullins JI. Feline leukemia virus infection and diseases. J Am Vet Med Assoc. 1991, 199(10):1287-1297.
5. Flynn JN, Dunham SP, Watson V, Jarrett O. Longitudinal analysis of feline leukemia virus-specific cytotoxic T lymphocytes: correlation with recovery from infection. J Virol 2002, 76(5):2306-2315. 10.1128/jvi.76.5.2306-2315.2002, 135947, 11836409.
6. Hayes KA, Rojko JL, Tarr MJ, Polas PJ, Olsen RG, Mathes LE. Atypical localised viral expression in a cat with feline leukaemia. Vet Rec 1989, 124(13):344-346.
7. Pacitti AM, Jarrett O, Hay D. Transmission of feline leukaemia virus in the milk of a non-viraemic cat. Vet Rec 1986, 118(14):381-384.
8. Madewell BR, Jarrett O. Recovery of feline leukaemia virus from non-viraemic cats. Vet Rec 1983, 112(15):339-342.
9. Rojko JL, Hoover EA, Quackenbush SL, Olsen RG. Reactivation of latent feline leukaemia virus infection. Nature 1982, 298(5872):385-388. 10.1038/298385a0, 6283387.
10. Post JE, Warren L. Reactivation of latent feline leukemia virus. Feline Leukemia Virus 1980, 151-155. New York: Elsevier North Holland Inc, Hardy WD Jr, Essex M, McClelland AJ.
11. Pedersen NC, Meric SM, Johnson L, Plucker S, Theiler GH. The clinical significance of latent feline leukemia virus infection in cats. Feline Pratice 1984, 14:32-48.
12. Pacitti AM, Jarrett O. Duration of the latent state in feline leukaemia virus infections. Vet Rec 1985, 117(18):472-474.
13. Hofmann-Lehmann R, Huder JB, Gruber S, Boretti F, Sigrist B, Lutz H. Feline leukaemia provirus load during the course of experimental infection and in naturally infected cats. J Gen Virol 2001, 82(Pt 7):1589-1596.
14. Torres AN, Mathiason CK, Hoover EA. Re-examination of feline leukemia virus: host relationships using real-time PCR. Virology 2005, 332(1):272-283. 10.1016/j.virol.2004.10.050, 15661159.
15. Tandon R, Cattori V, Gomes-Keller MA, Meli ML, Golder MC, Lutz H, Hofmann-Lehmann R. Quantitation of feline leukaemia virus viral and proviral loads by TaqMan real-time polymerase chain reaction. J Virol Methods 2005, 130(1-2):124-132. 10.1016/j.jviromet.2005.06.017, 16054243.
16. Hofmann-Lehmann R, Cattori V, Tandon R, Boretti FS, Meli ML, Riond B, Lutz H. How molecular methods change our views of FeLV infection and vaccination. Vet Immunol Immunopathol 2008, 123(1-2):119-123. 10.1016/j.vetimm.2008.01.017, 18295346.
17. Hofmann-Lehmann R, Cattori V, Tandon R, Boretti FS, Meli ML, Riond B, Pepin AC, Willi B, Ossent P, Lutz H. Vaccination against the feline leukaemia virus: outcome and response categories and long-term follow-up. Vaccine 2007, 25(30):5531-5539. 10.1016/j.vaccine.2006.12.022, 17240486.
18. Hofmann-Lehmann R, Tandon R, Boretti FS, Meli ML, Willi B, Cattori V, Gomes-Keller MA, Ossent P, Golder MC, Flynn JN, Lutz H. Reassessment of feline leukaemia virus (FeLV) vaccines with novel sensitive molecular assays. Vaccine 2006, 24(8):1087-1094. 10.1016/j.vaccine.2005.09.010, 16198454.
19. Lehmann R, Franchini M, Aubert A, Wolfensberger C, Cronier J, Lutz H. Vaccination of cats experimentally infected with feline immunodeficiency virus, using a recombinant feline leukemia virus vaccine. J Am Vet Med Assoc 1991, 199(10):1446-1452.
20. Hofmann-Lehmann R, Holznagel E, Ossent P, Lutz H. Parameters of disease progression in long-term experimental feline retrovirus (feline immunodeficiency virus and feline leukemia virus) infections: Hematology, clinical chemistry and lymphocyte subsets. Clin Diagn Lab Immunol 1997, 4(1):33-42. 170472, 9008278.
21. Lutz H, Pedersen NC, Durbin R, Theilen GH. Monoclonal antibodies to three epitopic regions of feline leukemia virus p27 and their use in enzyme-linked immunosorbent assay of p27. J Immunol Methods 1983, 56(2):209-220. 10.1016/0022-1759(83)90413-1, 6186744.
22. Calzolari M, Young E, Cox D, Davis D, Lutz H. Serological diagnosis of feline immunodeficiency virus infection using recombinant transmembrane glycoprotein. Vet Immunol Immunopathol 1995, 46(1-2):83-92. 10.1016/0165-2427(94)07008-U, 7618262.
23. Hofmann-Lehmann R, Holznagel E, Aubert A, Ossent P, Reinacher M, Lutz H. Recombinant FeLV vaccine: long-term protection and effect on course and outcome of FIV infection. Vet Immunol Immunopathol 1995, 46(1-2):127-137. 10.1016/0165-2427(94)07012-V, 7618252.
24. Reinacher M, Theilen G. Frequency and significance of feline leukemia virus infection in necropsied cats. Am J Vet Res 1987, 48(6):939-945.
25. Kipar A, Kremendahl J, Grant CK, von Bothmer I, Reinacher M. Expression of viral proteins in feline leukemia virus-associated enteritis. Vet Pathol 2000, 37(2):129-136. 10.1354/vp.37-2-129, 10714641.
26. Monteith CE, Chelack BJ, Davis WC, Haines DM. Identification of monoclonal antibodies for immunohistochemical staining of feline B lymphocytes in frozen and formalin-fixed paraffin-embedded tissues. Can J Vet Res 1996, 60(3):193-198. 1263832, 8809382.
27. Kessler Y, Helfer-Hungerbuehler AK, Cattori V, Meli ML, Zellweger B, Ossent P, Riond B, Reusch CE, Lutz H, Hofmann-Lehmann R. Quantitative TaqMan(R) real-time PCR assays for gene expression normalisation in feline tissues. BMC Mol Biol 2009, 10(1):106. 10.1186/1471-2199-10-106, 2803789, 20003366.
28. Cattori V, Tandon R, Riond B, Pepin AC, Lutz H, Hofmann-Lehmann R. The kinetics of feline leukaemia virus shedding in experimentally infected cats are associated with infection outcome. Vet Microbiol 2009, 133(3):292-296. 10.1016/j.vetmic.2008.07.001, 18774240.
29. Meli ML, Cattori V, Martinez F, Lopez G, Vargas A, Simon MA, Zorrilla I, Munoz A, Palomares F, Lopez-Bao JV, Pastor J, Tandon R, Willi B, Hofmann-Lehmann R, Lutz H. Feline leukemia virus and other pathogens as important threats to the survival of the critically endangered Iberian lynx (Lynx pardinus). PLoS ONE 2009, 4(3):e4744. 10.1371/journal.pone.0004744, 2649436, 19270739.
30. Cattori V, Tandon R, Pepin A, Lutz H, Hofmann-Lehman R. Rapid detection of feline leukemia virus provirus integration into feline genomic DNA. Mol Cell Probes 2006, 20(3-4):172-181. 10.1016/j.mcp.2005.11.007, 16488115.
31. Leutenegger CM, Mislin CN, Sigrist B, Ehrengruber MU, Hofmann-Lehmann R, Lutz H. Quantitative real-time PCR for the measurement of feline cytokine mRNA. Vet Immunol Immunopathol 1999, 71:291-305. 10.1016/S0165-2427(99)00100-2, 10587308.
32. Leutenegger CM, Klein D, Hofmann-Lehmann R, Mislin C, Hummel U, Boni J, Boretti F, Guenzburg WH, Lutz H. Rapid feline immunodeficiency virus provirus quantitation by polymerase chain reaction using the TaqMan fluorogenic real-time detection system. J Virol Methods. 1999, 78(1-2):105-116.
33. Cattori V, Hofmann-Lehmann R. Absolute quantitation of feline leukemia virus proviral DNA and viral RNA loads by TaqMan real-time PCR and RT-PCR. Methods Mol Biol 2008, 429:73-87. full_text, 18695960.
34. Allenspach K, Amacker M, Leutenegger CM, Hottiger M, Hofmann-Lehmann R, Hübscher U, Pistello M, Lutz H. Quantification of proviral FIV DNA using competitive PCR. Schweiz Arch Tierheilkd 1996, 138:87-92.
35. Hofmann-Lehmann R, Williams AL, Swenerton RK, Li PL, Rasmussen RA, Chenine AL, McClure HM, Ruprecht RM. Quantitation of simian cytokine and beta-chemokine mRNAs, using real-time reverse transcriptase-polymerase chain reaction: variations in expression during chronic primate lentivirus infection. AIDS Res Hum Retroviruses 2002, 18(9):627-639. 10.1089/088922202760019329, 12079558.
36. Tandon R, Cattori V, Willi B, Lutz H, Hofmann-Lehmann R. Quantification of endogenous and exogenous feline leukemia virus sequences by real-time PCR assays. Vet Immunol Immunopathol 2008, 123(1-2):129-133. 10.1016/j.vetimm.2008.01.027, 18295344.
37. Sheets RL, Pandey R, Jen WC, Roy-Burman P. Recombinant feline leukemia virus genes detected in naturally occurring feline lymphosarcomas. J Virol 1993, 67(6):3118-3125. 237649, 8388492.
38. Mathes LE, Pandey R, Chakrabarti R, Hofman FM, Hayes KA, Stromberg P, Roy-Burman P. Pathogenicity of a subgroup C feline leukemia virus (FeLV) is augmented when administered in association with certain FeLV recombinants. Virology 1994, 198:185-195. 10.1006/viro.1994.1021, 8259654.
39. Büttner A. Vergleichende Untersuchungen über Translation und Transkription von Strukturproteinen des Felinen Leukämievirus nach experimenteller Infektion. Inaugural dissertation 2004, Giessen, Germany: University of Giessen.
40. Donahue PR, Hoover EA, Beltz GA, Riedel N, Hirsch VM, Overbaugh J, Mullins JI. Strong sequence conservation among horizontally transmissible, minimally pathogenic feline leukemia viruses. J Virol 1988, 62:722-731. 253625, 2828667.
41. Nishigaki K, Okuda M, Endo Y, Watari T, Tsujimoto H, Hasegawa A. Structure and function of the long terminal repeats of feline leukemia viruses derived from naturally occurring acute myeloid leukemias in cats. J Virol 1997, 71(12):9823-9827. 230298, 9371654.
42. Kumar S, Nei M, Dudley J, Tamura K. MEGA: a biologist-centric software for evolutionary analysis of DNA and protein sequences. Brief Bioinform 2008, 9(4):299-306. 10.1093/bib/bbn017, 2562624, 18417537.
43. Thompson JD, Higgins DG, Gibson TJ. CLUSTAL W: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice. Nucleic Acids Res 1994, 22(22):4673-4680. 10.1093/nar/22.22.4673, 308517, 7984417.
44. Felsenstein J. Confidence Limits on Phylogenies: An Approach Using the Bootstrap. Evolution 1985, 39(4):783-791.
45. Nei M, Kumar S. Molecular Evolution and Phylogenetics. 2000, New York: Oxford University Press.
46. Werner JA, Woo JC, Vernau W, Graham PS, Grahn RA, Lyons LA, Moore PF. Characterization of feline immunoglobulin heavy chain variable region genes for the molecular diagnosis of B-cell neoplasia. Vet Pathol 2005, 42(5):596-607. 10.1354/vp.42-5-596, 16145206.
47. Moore PF, Woo JC, Vernau W, Kosten S, Graham PS. Characterization of feline T cell receptor gamma (TCRG) variable region genes for the molecular diagnosis of feline intestinal T cell lymphoma. Vet Immunol Immunopathol 2005, 106(3-4):167-178. 10.1016/j.vetimm.2005.02.014, 15963816.
48. Henrich M, Hecht W, Weiss AT, Reinacher M. A new subgroup of immunoglobulin heavy chain variable region genes for the assessment of clonality in feline B-cell lymphomas. Vet Immunol Immunopathol 2009, 130(1-2):59-69. 10.1016/j.vetimm.2009.01.006, 19243841.
49. Greiner M. Serodiagnostische Tests. Evaluierung und Interpretation in der Veterinärmedizin und anderen Fachgebieten Springer, Berlin, Germany 2003,
50. Landis JR, Koch GG. The measurement of observer agreement for categorical data. Biometrics 1977, 33(1):159-174. 10.2307/2529310, 843571.
51. Valli VE, Jacobs RM, Parodi AL, Vernau W, Moore PF. World Health Organization International Histological Classification of Tumors of Domestic Animals: Histological Classification of Hematopoietic Tumors of Domestic Animals. 2002, VIII. Armed Force Institute of Pathology, American Registry of Pathology.
52. Rey MA, Prasad R, Tailor CS. The C domain in the surface envelope glycoprotein of subgroup C feline leukemia virus is a second receptor-binding domain. Virology 2008, 370(2):273-284. 10.1016/j.virol.2007.09.011, 17945326.
53. Stewart MA, Warnock M, Wheeler A, Wilkie N, Mullins JI, Onions DE, Neil JC. Nucleotide sequences of a feline leukemia virus subgroup A envelope gene and long terminal repeat and evidence for the recombinational origin of subgroup B viruses. J Virol 1986, 58:825-834. 252989, 3009890.
54. Shelton GH, Grant CK, Cotter SM, Gardner MB, Hardy WD, DiGiacomo RF. Feline immunodeficiency virus and feline leukemia virus infections and their relationships to lymphoid malignancies in cats: a retrospective study (1968-1988). J Acquir Immune Defic Syndr 1990, 3:623-630.
55. Chandhasin C, Coan PN, Pandrea I, Grant CK, Lobelle-Rich PA, Puetter A, Levy LS. Unique long terminal repeat and surface glycoprotein gene sequences of feline leukemia virus as determinants of disease outcome. J Virol 2005, 79(9):5278-5287. 10.1128/JVI.79.9.5278-5287.2005, 1082761, 15827142.
56. Jarrett O. Pathogenicity of feline leukemia virus is commonly associated with variant viruses. Leukemia 1992, 6(Suppl 3):153S-154S.
57. Neil JC, Fulton R, Rigby M, Stewart M. Feline leukaemia virus: generation of pathogenic and oncogenic variants. Curr Top Microbiol Immunol 1991, 171:67-93.
58. Shelton GH, McKim KD, Cooley PL, Dice PF, Russell RG, Grant CK. Feline leukemia virus and feline immunodeficiency virus infections in a cat with lymphoma. J Am Vet Med Assoc 1989, 194(2):249-252.
59. Callanan JJ, Jones BA, Irvine J, Willett BJ, McCandlish IA, Jarrett O. Histologic classification and immunophenotype of lymphosarcomas in cats with naturally and experimentally acquired feline immunodeficiency virus infections. Vet Pathol 1996, 33(3):264-272.
60. Poli A, Abramo F, Baldinotti F, Pistello M, Da Prato L, Bendinelli M. Malignant lymphoma associated with experimentally induced feline immunodeficiency virus infection. J Comp Pathol 1994, 110:319-328. 10.1016/S0021-9975(08)80309-X, 7914523.
61. Gabor LJ, Love DN, Malik R, Canfield PJ. Feline immunodeficiency virus status of Australian cats with lymphosarcoma. Aust Vet J 2001, 79(8):540-545. 10.1111/j.1751-0813.2001.tb10742.x, 11599813.
62. Beatty JA, Callanan JJ, Terry A, Jarrett O, Neil JC. Molecular and immunophenotypical characterization of a feline immunodeficiency virus (FIV)-associated lymphoma: a direct role for FIV in B-lymphocyte transformation?. J Virol 1998, 72(1):767-771. 109433, 9420284.
63. Feichtinger H, Putkonen P, Parravicini C, Li SL, Kaaya EE, Bottiger D, Biberfeld G, Biberfeld P. Malignant lymphomas in cynomolgus monkeys infected with simian immunodeficiency virus. Am J Pathol 1990, 137(6):1311-1315. 1877739, 1701962.
64. Levine AM. Acquired immunodeficiency syndrome-related lymphoma. Blood 1992, 80(1):8-20.
65. Jackson ML, Wood SL, Misra V, Haines DM. Immunohistochemical identification of B and T lymphocytes in formalin-fixed, paraffin-embedded feline lymphosarcomas: relation to feline leukemia virus status, tumor site, and patient age. Can J Vet Res 1996, 60(3):199-204. 1263833, 8809383.
66. Phipps AJ, Chen H, Hayes KA, Roy-Burman P, Mathes LE. Differential pathogenicity of two feline leukemia virus subgroup A molecular clones, pFRA and pF6A. J Virol 2000, 74(13):5796-5801. 10.1128/JVI.74.13.5796-5801.2000, 112073, 10846058.
67. Beatty JA, Lawrence CE, Callanan JJ, Grant CK, Gault EA, Neil JC, Jarrett O. Feline immunodeficiency virus (FIV)-associated lymphoma: a potential role for immune dysfunction in tumourigenesis. Vet Immunol Immunopathol 1998, 65(2-4):309-322. 10.1016/S0165-2427(98)00164-0, 9839882.
68. Hamilton-Dutoit SJ, Pallesen G, Franzmann MB, Karkov J, Black F, Skinhoj P, Pedersen C. AIDS-related lymphoma. Histopathology, immunophenotype, and association with Epstein-Barr virus as demonstrated by in situ nucleic acid hybridization. Am J Pathol 1991, 138(1):149-163. 1886047, 1846263.
69. Overbaugh J, Bangham CR. Selection forces and constraints on retroviral sequence variation. Science 2001, 292(5519):1106-1109. 10.1126/science.1059128, 11352065.
70. Jarrett O, Russell PH. Differential growth and transmission in cats of feline leukaemia viruses of subgroups A and B. Int J Cancer 1978, 21:466-472. 10.1002/ijc.2910210411, 208983.
71. Overbaugh J, Riedel N, Hoover EA, Mullins JI. Transduction of endogenous envelope genes by feline leukaemia virus in vitro. Nature 1988, 332:731-734. 10.1038/332731a0, 2895894.
72. Rigby MA, Rojko JL, Stewart MA, Kociba GJ, Cheney CM, Rezanka LJ, Mathes LE, Hartke JR, Jarrett O, Neil JC. Partial dissociation of subgroup C phenotype and in vivo behaviour in feline leukaemia viruses with chimeric envelope genes. J Gen Virol 1992, 73:2839-2847. 10.1099/0022-1317-73-11-2839, 1331290.
73. Sarangi A, Bupp K, Roth MJ. Identification of a retroviral receptor used by an envelope protein derived by peptide library screening. Proc Natl Acad Sci USA 2007, 104(26):11032-11037. 10.1073/pnas.0704182104, 1904118, 17581869.
74. Mazari PM, Linder-Basso D, Sarangi A, Chang Y, Roth MJ. Single-round selection yields a unique retroviral envelope utilizing GPR172A as its host receptor. Proc Natl Acad Sci USA 2009, 106(14):5848-5853. 10.1073/pnas.0809741106, 2667028, 19307586.
75. Bupp K, Sarangi A, Roth MJ. Probing sequence variation in the receptor-targeting domain of feline leukemia virus envelope proteins with peptide display libraries. J Virol 2005, 79(3):1463-1469. 10.1128/JVI.79.3.1463-1469.2005, 544108, 15650172.
76. Chandhasin C, Coan PN, Levy LS. Subtle mutational changes in the SU protein of a natural feline leukemia virus subgroup A isolate alter disease spectrum. J Virol 2005, 79(3):1351-1360. 10.1128/JVI.79.3.1351-1360.2005, 544135, 15650161.
77. Jackson ML, Haines DM, Misra V. Sequence analysis of the putative viral enhancer in tissues from 33 cats with various feline leukemia virus-related diseases. Vet Microbiol 1996, 53(3-4):213-225. 10.1016/S0378-1135(96)01228-X, 9008333.
78. Fulton R, Plumb M, Shield L, Neil JC. Structural diversity and nuclear protein binding sites in the long terminal repeats of feline leukemia virus. J Virol 1990, 64:1675-1682. 249304, 2157050.
79. Matsumoto Y, Momoi Y, Watari T, Goitsuka R, Tsujimoto H, Hasegawa A. Detection of enhancer repeats in the long terminal repeats of feline leukemia viruses from cats with spontaneous neoplastic and nonneoplastic diseases. Virology 1992, 189:745-749. 10.1016/0042-6822(92)90598-J, 1322598.
80. Miura T, Shibuya M, Tsujimoto H, Fukasawa M, Hayami M. Molecular cloning of a feline leukemia provirus integrated adjacent to the c-myc gene in a feline T-cell leukemia cell line and the unique structure of its long terminal repeat. Virology 1989, 169:458-461. 10.1016/0042-6822(89)90172-4, 2539700.
81. Levy LS. Advances in understanding molecular determinants in FeLV pathology. Vet Immunol Immunopathol 2008, 123(1-2):14-22. 10.1016/j.vetimm.2008.01.008, 2413067, 18289704.
82. Francis DP, Essex M, Hardy WD. Excretion of feline leukaemia virus by naturally infected pet cats. Nature 1977, 269:252-254. 10.1038/269252a0, 201852.
83. Elder JH, Mullins JI. Nucleotide sequence of the envelope gene of Gardner-Arnstein feline leukemia virus B reveals unique sequence homologies with a murine mink cell focus-forming virus. J Virol 1983, 46:871-880. 256562, 6304347.