Assay and heterologous expression in Pichia pastoris of plant cell wall type-II membrane anchored glycosyltransferases

Glycoconjugate Journal

Volumn 26, Issue 9, 2009, Pages 1235-1246

  Petersen, Bent Larsen ^a   Egelund, Jack ^b   Damager, Iben ^a   Faber, Kirsten ^a   Krüger Jensen, Jacob ^b   Yang, Zhang ^a   Bennett, Eric Paul ^b   Scheller, Henrik Vibe ^c   Ulvskov, Peter ^a  

^a AARHUS UNIVERSITY   (Denmark)

^b UNIVERSITY OF COPENHAGEN   (Denmark)

^c LAWRENCE BERKELEY NATIONAL LABORATORY   (United States)

Author keywords

Free sugar assay; Glycosyltransferase; Pichia pastoris; Plant cell wall; Protein expression

Indexed keywords

DIVALENT CATION; FUCOSE; GLUCAN SYNTHASE; GLYCOSYLATED PROTEIN; GLYCOSYLTRANSFERASE; GLYCOSYLTRANSFERASE RGXT1; GLYCOSYLTRANSFERASE RGXT2; MANGANESE; UNCLASSIFIED DRUG; URIDINE DIPHOSPHATE XYLOSE; XYLOSE; ARABIDOPSIS PROTEIN; GLYCOPROTEIN;

AMINO TERMINAL SEQUENCE; ARABIDOPSIS; ARTICLE; BACULOVIRUS; CARBOXY TERMINAL SEQUENCE; CELL MEMBRANE; CELL WALL; CONTROLLED STUDY; DEGLYCOSYLATION; DIALYSIS; ELECTROSPRAY MASS SPECTROMETRY; ENZYME ACTIVITY; GENE EXPRESSION SYSTEM; HETEROLOGOUS EXPRESSION; INSECT CELL; NONHUMAN; PH MEASUREMENT; PICHIA PASTORIS; PLANT CELL; PRIORITY JOURNAL; PROTEIN GLYCOSYLATION; PROTEIN PURIFICATION; PROTEIN SECRETION; TURNOVER TIME; AMINO ACID SEQUENCE; ANIMAL; CYTOLOGY; DRUG EFFECT; ENZYME ACTIVATION; ENZYME ASSAY; ENZYMOLOGY; GLYCOSYLATION; IMMUNOBLOTTING; INSECT; METABOLISM; METHODOLOGY; MOLECULAR GENETICS; NUCLEOTIDE SEQUENCE; PH; PICHIA; SECRETION;

ARABIDOPSIS; HEXAPODA; PICHIA; PICHIA PASTORIS;

AMINO ACID SEQUENCE; ANIMALS; ARABIDOPSIS; ARABIDOPSIS PROTEINS; BASE SEQUENCE; CATIONS, DIVALENT; CELL MEMBRANE; CELL WALL; ENZYME ACTIVATION; ENZYME ASSAYS; GLYCOPROTEINS; GLYCOSYLATION; GLYCOSYLTRANSFERASES; HYDROGEN-ION CONCENTRATION; IMMUNOBLOTTING; INSECTS; MOLECULAR SEQUENCE DATA; PICHIA; SPECTROMETRY, MASS, ELECTROSPRAY IONIZATION;

EID: 77649262826     PISSN: 02820080     EISSN: 15734986     Source Type: Journal    
DOI: 10.1007/s10719-009-9242-0     Document Type: Article

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