Expression, purification, and characterization of a human recombinant 17β-hydroxysteroid dehydrogenase type 1 in escherichia coli

Molecular Biotechnology

Volumn 44, Issue 2, 2010, Pages 133-139

  Chang, Yi Hsun ^a   Wang, Yuan Liang ^a   Lin, Jain Yu ^a   Chuang, Lea Yea ^a   Hwang, Chi Ching ^a  

^a KAOHSIUNG MEDICAL UNIVERSITY   (Taiwan)

Author keywords

17 Hydroxysteroid dehydrogenase 1; Enzyme catalysis; Protein expression; Protein purification; Short chain dehydrogenase reductase

Indexed keywords

BACTERIAL EXPRESSION SYSTEMS; BIOLOGICAL ACTIVITIES; COFACTORS; EFFICIENT METHOD; ENZYME CATALYSIS; EXPRESSION VECTORS; HYDROXYSTEROID DEHYDROGENASE; KINETIC STUDY; PROTEIN EXPRESSIONS; PROTEIN PURIFICATION; SEX STEROIDS; SINGLE-STEP;

AFFINITY CHROMATOGRAPHY; CATALYSIS; CELL CULTURE; CHROMATOGRAPHIC ANALYSIS; DEHYDROGENATION; ENZYMES; ESCHERICHIA COLI; GENE EXPRESSION; HORMONES;

PURIFICATION;

ESTRADIOL; NICOTINAMIDE ADENINE DINUCLEOTIDE PHOSPHATE; RECOMBINANT ENZYME; TESTOSTERONE 17BETA DEHYDROGENASE; TESTOSTERONE 17BETA DEHYDROGENASE TYPE 1; UNCLASSIFIED DRUG;

ARTICLE; BACTERIAL CELL; CELL TRANSFORMATION; CONTROLLED STUDY; ENZYME KINETICS; ENZYME PURIFICATION; ENZYME STABILITY; ESCHERICHIA COLI; EXPRESSION VECTOR; GENE EXPRESSION SYSTEM; GENE OVEREXPRESSION; NONHUMAN; OXIDATION; PH; PLASMID; PROTEIN EXPRESSION; STEADY STATE; THERMOSTABILITY;

17-HYDROXYSTEROID DEHYDROGENASES; ELECTROPHORESIS, POLYACRYLAMIDE GEL; ESCHERICHIA COLI; HUMANS; RECOMBINANT PROTEINS;

BACTERIA (MICROORGANISMS); ESCHERICHIA COLI;

EID: 77649238687     PISSN: 10736085     EISSN: None     Source Type: Journal    
DOI: 10.1007/s12033-009-9221-5     Document Type: Article

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