Effects of environmental factors on in vivo folding of Bacillus macerans cyclodextrin glycosyltransferase in recombinant Escherichia coli

Journal of Microbiology and Biotechnology

Volumn 11, Issue 1, 2001, Pages 92-96

  Jin, H H ^a   Han, N S ^a   Kweon, D H ^a   Park, Y C ^a   Seo, J H ^a  

^a Seoul National University   (South Korea)

Author keywords

Cyclodextrin glycosyltransferase; Environmental factors; In vivo folding; Inclusion bodies; Recombinant E. coli

Indexed keywords

BETAINE; CALCIUM CHLORIDE; CYCLOMALTODEXTRIN GLUCANOTRANSFERASE; SODIUM CHLORIDE;

ARTICLE; BACILLUS; BACILLUS MACERANS; CELL FRACTIONATION; CELL GROWTH; CONTROLLED STUDY; CULTURE MEDIUM; ENVIRONMENTAL FACTOR; ENZYME ACTIVITY; ESCHERICHIA COLI; GENETIC RECOMBINATION; IN VIVO STUDY; NONHUMAN; OSMOTIC PRESSURE; PH; PROTEIN EXPRESSION; PROTEIN FOLDING; TEMPERATURE;

BACILLUS MACERANS; ESCHERICHIA COLI;

EID: 0035091270     PISSN: 10177825     EISSN: None     Source Type: Journal    
DOI: None     Document Type: Article

References (20)

1. Cyclodextrin-Glucanotransferase von Klebsiella pneumoniae MSal. Bedeuting des enzyme für metabolisms der cyclodextrine bei Klebsiella pneumoniae MSal
2. Purification of cyclodextrin glycosyltransferase by immunochromatography
3. Formation of aggregates from a thermolabile in vivo folding intermediate in P22 tailspike mutation
4. Expression of Bacillus macerans cyclodextrin glycosyltransferase in Bacillus subtilis
5. Structure of cyclodextrin glycosyltransferase refined at 2.0 Å resolution
6. Catalytic center of cyclodextrin glycosyltransferase derived from X-ray structure analysis combined with site-directed mutagenesis
7. Protein aggregation kinetics in an Escherichia coli strain overexpressing a Salmonella typhimurium CheY mutant gene
8. Expression of the EPO-like domains of human thrombopoietin in Escherichia coli
9. Multimeric expression of the antimicrobial peptide buforin II in Escherichia coli by fusion to a cysteine-rich acidic peptide
10. High-level expression of cyclodextrin glycosyltransferase in E. coli using a T7 promoter expression system
11. A spectrophotometric assay for the cyclization activity of cyclomaltohesaose (α-cyclodextrin) glucanotransferase
12. Adaptation of Escherichia coli to high osmolarity environments: Osmoregulation of the high-affinity glycine betaine transport system ProU
13. T7 lysozyme inhibits transcription by T7 RNA polymerase
14. Characterization of the β-cyclodextrin glucanotransferase gene of Bacillus firmus var. alkalophilus and its expression in E. coli
15. Expression of cyclodextrin glucanotransferase from Bacillus macerans in recombinant Escherichia coli
16. Fed-batch fermentation of recombinant Escherichia coli to produce Bacillus macerans CGTase
17. Expression of soluble and fully functional ricin A chain in Escherichia coli is temperature-sensitive
18. Higher culture pH is preferable for inclusion body formation of recombinant salmon growth hormone in Escherichia coli
19. Transient accumulation of glycine betaine and dynamics of endogenous osmolytes in salt-stressed cultures of Sinorhizobium meliloti
20. Inclusion body formation by interleukin-1β depends on the thermal sensitivity of a folding intermediate