Construction of Pichia pastoris strain expressing salivary plasminogen activator from vampire bat (Desmodus rotundus)

Shengwu Gongcheng Xuebao/Chinese Journal of Biotechnology

Volumn 25, Issue 4, 2009, Pages 566-574

  Liu, Yan ^a   Su, Chang ^a   Song, Xiaoshuang ^a   Tang, Yalan ^b   Bao, Zhenhong ^b  

^a SOUTHWEST UNIVERSITY   (China)

^b NANJING UNIVERSITY   (China)

Author keywords

Fibrin plate assay; High expression; Pichia pastoris; Plasminogen activator; Vampire bat

Indexed keywords

ANIMALIA; CRICETINAE; CRICETULUS GRISEUS; DESMODUS ROTUNDUS; MAMMALIA; PICHIA PASTORIS;

PLASMINOGEN ACTIVATOR; RECOMBINANT PROTEIN; SALIVARY PLASMINOGEN ACTIVATOR ALPHA 1, DESMODUS ROTUNDUS;

ANIMAL; ARTICLE; BAT; BIOSYNTHESIS; DNA SEQUENCE; GENE VECTOR; GENETIC RECOMBINATION; GENETICS; METABOLISM; MOLECULAR GENETICS; NUCLEOTIDE SEQUENCE; PICHIA;

ANIMALS; BASE SEQUENCE; CHIROPTERA; GENETIC VECTORS; MOLECULAR SEQUENCE DATA; PICHIA; PLASMINOGEN ACTIVATORS; RECOMBINANT PROTEINS; RECOMBINATION, GENETIC; SEQUENCE ANALYSIS, DNA;

EID: 77649220796     PISSN: 10003061     EISSN: None     Source Type: Journal    
DOI: None     Document Type: Article

References (19)

1. Gardell SJ, Duong LT, Diehl RE, et al. Isolation, characterization, and cDNA cloning of a vampire bat salivary plasminogen activator. J Biol Chem, 1989, 264(30): 17947-17952.
2. Kratzschmar J, Haendler B, Langer G, et al. The plasminogen activator family from the salivary gland of the vampire bat Desmodus rotundus: Cloning and expression. Gene, 1991, 105: 229-237.
3. Kratzschmar J, Haendler B, Bringmann P, et al. High-level secretion of the four salivary plasminogen activators from the vampire bat Desmodus rotundus by stably transfected baby hamster kidney cells. Gene, 1992, 116(2): 281-284.
4. Bergum PW, Gardell SJ. Vampire bat salivary plasminogen activator exhibits a strict and fastidious requirement for polymeric fibrin as its cofactor, unlike human tissue-type plasminogen activator: A kinetic analysis. J Biol Chem, 1992, 267(25): 17726-17731.
5. Gardell SJ, Hare TR, Bergum PW, et al. Vampire bat salivary plasminogen activator is quiescent in human plasma in the absence of fibrin unlike human tissue plasminogen activator. Blood, 1990, 76(12): 2560-2564.
6. Hare TR, Gardell SJ. Vampire bat salivary plasminogen activator promotes robust lysis of plasma clots in a plasma milieu without causing fluid phase plasminogen activation. Thromb Haemost, 1992, 68: 165-169.
7. Petri T, Langer G, Bringmann P, et al. Production of vampire bat plasminogen-activator DSPA alfa1 in CHO and insect cells. J Biotechnol, 1995, 39(1): 75-83.
8. Verstraete M, Lijnen HR, Collen D. Thrombolytic agents in development. Drugs, 1995, 50: 29-42.
9. Liberatore GT, Samson A, Bladin C, et al. Vampire bat salivary plasminogen activator (desmoteplase) a unique fibrinolytic enzyme that does not promote neurodegeneration. Stroke, 2003, 34(2): 537-543.
10. Reddrop C, Moldrich RX, Beart PM, et al. Vampire bat salivary plasminogen activator (desmoteplase) inhibits tissue-type plasminogen activator-induced potentiation of excitotoxic injury. Stroke, 2005, 36(6): 1241-1246.
11. Pugsley M, Petersen KU, Gupta S, et al. Recombinant desmodus salivary plasminogen activator (desmote- plase) in acute ischemic stroke. Stroke, 2006, 37(2): 656-656.
12. Sambrook J, Fritsch EF, Maniatis T. Molecular Cloning: A Laboratory Manual. 3nd Ed. New York: Cold Spring Harbor Laboratory Press, 2002.
13. Scorer CA, Clare JJ, McCombie WR. Rapid selection using G418 of high copy number transformants of Pichia pastoris for high-level foreign gene expression. Biotechnology, 1994, 12(2): 181-184.
14. Bradford MM. A rapid and sensitve method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem, 1976, 72: 248.
15. Marsh NA, Gaffney PJ. Rapid fibrin plate-method for plasminogen activator assay. Thromb Haemostasis, 1977, 38(2): 545-551.
16. Siegei RS. Methylotroyphic yeast Pichia pastoris produced in high-cell-density fermentation with high cell yields as vehicle for recombinant protein production. Biotechnol Bioeng, 1989, 34: 403-404.
17. Borgheresi RA, Palma MS, Ducancel F, et al. Expression and processing of recombinant sarafotoxins precursor in Pichia pastoris. Toxicon, 2001, 39: 1211-1218.
18. Wei ZR, Wang YJ, Li GQ, et al. Optimized gene synthesis, expression and purification of active salivary plasminogen activator α2(DSPAα2)of Desmodus rotundus in Pichia pastoris. Protein Expr Purif, 2008, 57(1): 27-33.
19. Pan X, Cao GW, Ke CW, et al. Structure and function of vascular endotheliol growth factor and its related proteins. Acta Biochim Biophys Sin, 1999, 31(4): 357-361.