Expression and processing of recombinant sarafotoxins precursor in Pichia pastoris

Toxicon

Volumn 39, Issue 8, 2001, Pages 1211-1218

  Borgheresi, Rosa A M B ^a   Palma, Mario S ^b   Ducancel, Frédéric ^c   Camargo, Antonio C M ^a   Carmona, Eurídice ^a  

^a INSTITUTO BUTANTAN   (Brazil)

^b SÃO PAULO STATE UNIVERSITY UNESP   (Brazil)

^c CEA SACLAY   (France)

Author keywords

Expression in Pichia pastoris; Precursor of sarafotoxins; Processing enzymes; Sarafotoxins

Indexed keywords

COMPLEMENTARY DNA; PROTEIN PRECURSOR; RECOMBINANT PROTEIN; SARAFOTOXIN; SNAKE VENOM;

ANIMAL TISSUE; ARTICLE; ATRACTASPIS; CONTROLLED STUDY; CULTURE MEDIUM; DRUG ACTIVITY; IMMUNOBLOTTING; MASS SPECTROMETRY; MOLECULAR CLONING; NONHUMAN; PICHIA PASTORIS; POLYACRYLAMIDE GEL ELECTROPHORESIS; PRIORITY JOURNAL; PROTEIN EXPRESSION; PROTEIN PROCESSING; RAT; SNAKE; TOXIN ANALYSIS;

ANIMALS; AORTA; CLONING, MOLECULAR; MASS SPECTROMETRY; PICHIA; PROTEIN PRECURSORS; RATS; RECOMBINANT PROTEINS; VIPER VENOMS;

ATRACTASPIS; BOTHROPS JARARACA; PICHIA PASTORIS; SERPENTES;

EID: 0035076004     PISSN: 00410101     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0041-0101(00)00265-8     Document Type: Article

References (28)

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2. The conformacional of purified Toxoplasma gonddi, SAG1, antigen, secreted from engineered Pichia pastoris, is adequate for serorecognition and cell proliferation
3. Yeast systems for the commercial production of heterologous proteins
4. High-level expression of tetanus toxin fragment C in Pichia pastoris containing multiple tandem integrations of the gene
5. Continuous production of a novel lysozyme via secretion from the yeast Pichia pastoris
6. Cloning and sequence analysis of cDNAs encoding precursor of sarafotoxins
7. Les sarafotoxines de venins de serpent
8. Facile production of native-like λ-bungarotoxin in yeast: An enhanced system for the production of a neuronal nicotinic acetylcholine receptor probe
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10. Cleavage of structural proteins during the assembly of head of bacteriophage T4
11. Biological activities of [Thr2]sarafotoxin-b, a synthetic analogue of sarafotoxin-b
12. High-level secretion and very efficient isotopic labeling of tick anticoagulant peptide (TAP) expressed in the methylotrophic yeast Pichia pastoris
13. Processing of the Alzheimer's disease amyloid precursor protein in Pichia pastoris: Immunodetection of α-, β- and γ-secretase products
14. Atypical processing of amyloid precursor fusion protein by proteolytic activity in Pichia pastoris
15. Expression and processing of vertebrate acetylcholinesterase in the yeast Pichia pastoris
16. Recombinant human mast cell tryptase: Stable expression in Pichia pastoris and purification of fully active enzyme
17. Cyclic guanosine monophospate inhibition of contraction may be mediated through inhibition of phosphatidylinositol hidrolysis in rat aorta
18. Sarafotoxin expression in the bronchopulmonary tract: Immunohistochemical occurrence and colocalization with endothelins
19. Endothelin ETA and ETB receptors mediate vascular smooth muscle contraction
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21. Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: Procedure and some applications
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23. Endothelin: From molecule to man
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25. Processing, activity, and inhibition of recombinant cyprosin, an aspartic proteinase from cardoon (Cyanara cardunculus)
26. Cleavage-secretion of angiotensin I-converting enzyme in yeast
27. Effects of catecholamines on the isolated aorta of the snake Bothrops jararaca
28. Activity of sarafotoxin/endothelin peptides in the heart and brain of lower vertebrates