Luminescence detection of SNARE-SNARE interaction in Arabidopsis protoplasts

Plant Molecular Biology

Volumn 72, Issue 4, 2010, Pages 433-444

  Kato, Naohiro ^a   Fujikawa, Yukichi ^a   Fuselier, Taylor ^a   Adamou Dodo, Rimanatou ^b   Nishitani, Aiko ^c   Sato, Masa H ^c  

^a LOUISIANA STATE UNIVERSITY   (United States)

^b UNIVERSITY OF LOUISIANA AT MONROE   (United States)

^c KYOTO PREFECTURAL UNIVERSITY   (Japan)

Author keywords

Arabidopsis; High throughput; Interaction; SNARE; Split luciferase complementation

Indexed keywords

ARABIDOPSIS; EMBRYOPHYTA; EUKARYOTA;

ARABIDOPSIS PROTEIN; GREEN FLUORESCENT PROTEIN; HYBRID PROTEIN; PEN1 PROTEIN, ARABIDOPSIS; QB SNARE PROTEIN; SNARE PROTEIN; SYNTAXIN; SYP61 PROTEIN, ARABIDOPSIS; VAM3 PROTEIN, ARABIDOPSIS; VESICLE TRANSPORT V SNARE 11 PROTEIN, ARABIDOPSIS; VESICLE TRANSPORT V-SNARE 11 PROTEIN, ARABIDOPSIS;

AMINO ACID SUBSTITUTION; ARABIDOPSIS; ARTICLE; CHEMISTRY; GENETIC COMPLEMENTATION; GENETICS; LUMINESCENCE; METABOLISM; PHOTOTROPHY; PROTEIN DOMAIN; PROTOPLAST; TRANSGENIC PLANT;

AMINO ACID SUBSTITUTION; ARABIDOPSIS; ARABIDOPSIS PROTEINS; GENETIC COMPLEMENTATION TEST; GREEN FLUORESCENT PROTEINS; LUMINESCENT MEASUREMENTS; PHOTOTROPHIC PROCESSES; PLANTS, GENETICALLY MODIFIED; PROTEIN INTERACTION DOMAINS AND MOTIFS; PROTOPLASTS; QA-SNARE PROTEINS; QB-SNARE PROTEINS; RECOMBINANT FUSION PROTEINS; SNARE PROTEINS;

EID: 77449160621     PISSN: 01674412     EISSN: None     Source Type: Journal    
DOI: 10.1007/s11103-009-9581-z     Document Type: Article

References (44)

1. An SJ, Almers W (2004) Tracking SNARE complex formation in live endocrine cells. Science 306: 1042-1046.
2. Antonin W, Fasshauer D, Becker S, Jahn R, Schneider TR (2002) Crystal structure of the endosomal SNARE complex reveals common structural principles of all SNAREs. Nat Struct Biol 9: 107-111.
3. Bethani I, Lang T, Geumann U, Sieber JJ, Jahn R, Rizzoli SO (2007) The specificity of SNARE pairing in biological membranes is mediated by both proof-reading and spatial segregation. EMBO J 26: 3981-3992.
4. Chen Z, Noir S, Kwaaitaal M, Hartmann HA, Wu MJ, Mudgil Y, Sukumar P, Muday G, Panstruga R, Jones AM (2009) Two-seven-transmembrane domain MILDEW RESISTANCE LOCUS O proteins cofunction in Arabidopsis root thigmomorphogenesis. Plant Cell 21: 1972-1991.
5. Dulubova I, Yamaguchi T, Gao Y, Min SW, Huryeva I, Sudhof TC, Rizo J (2002) How Tlg2p/syntaxin 16 'snares' Vps45. EMBO J 21: 3620-3631.
6. Ebine K, Okatani Y, Uemura T, Goh T, Shoda K, Niihama M, Morita MT, Spitzer C, Otegui MS, Nakano A, Ueda T (2008) A SNARE complex unique to seed plants is required for protein storage vacuole biogenesis and seed development of Arabidopsis thaliana. Plant Cell 20: 3006-3021.
7. Enami K, Ichikawa M, Uemura T, Kutsuna N, Hasezawa S, Nakagawa T, Nakano A, Sato MH (2009) Differential expression control and polarized distribution of plasma membrane-resident SYP1 SNAREs in Arabidopsis thaliana. Plant Cell Physiol 50: 280-289.
8. Fisher RJ, Pevsner J, Burgoyne RD (2001) Control of fusion pore dynamics during exocytosis by Munc18. Science 291: 875-878.
9. Foresti O, daSilva LL, Denecke J (2006) Overexpression of the Arabidopsis syntaxin PEP12/SYP21 inhibits transport from the prevacuolar compartment to the lytic vacuole in vivo. Plant Cell 18: 2275-2293.
10. Fujikawa Y, Kato N (2007) Split luciferase complementation assay to study protein-protein interactions in Arabidopsis protoplasts. Plant J 52: 185-195.
11. Geelen D, Leyman B, Batoko H, Di Sansebastiano GP, Moore I, Blatt MR (2002) The abscisic acid-related SNARE homolog NtSyr1 contributes to secretion and growth: evidence from competition with its cytosolic domain. Plant Cell 14: 387-406.
12. Hartmann U, Valentine WJ, Christie JM, Hays J, Jenkins GI, Weisshaar B (1998) Identification of UV/blue light-response elements in the Arabidopsis thaliana chalcone synthase promoter using a homologous protoplast transient expression system. Plant Mol Biol 36: 741-754.
13. Hong W (2005) SNAREs and traffic. Biochim Biophys Acta 1744: 493-517.
14. Jahn R, Scheller RH (2006) SNAREs-engines for membrane fusion. Nat Rev Mol Cell Biol 7: 631-643.
15. Jahn R, Lang T, Sudhof TC (2003) Membrane fusion. Cell 112: 519-533.
16. Kato N, Reynolds D, Brown ML, Boisdore M, Fujikawa Y, Morales A, Meisel LA (2008) Multidimensional fluorescence microscopy of multiple organelles in Arabidopsis seedlings. Plant Methods 4: 9.
17. Kim SB, Ozawa T, Watanabe S, Umezawa Y (2004) High-throughput sensing and noninvasive imaging of protein nuclear transport by using reconstitution of split Renilla luciferase. Proc Natl Acad Sci USA 101: 11542-11547.
18. Kwon C, Neu C, Pajonk S, Yun HS, Lipka U, Humphry M, Bau S, Straus M, Kwaaitaal M, Rampelt H, El Kasmi F, Jurgens G, Parker J, Panstruga R, Lipka V, Schulze-Lefert P (2008) Co-option of a default secretory pathway for plant immune responses. Nature 451: 835-840.
19. Leshem Y, Melamed-Book N, Cagnac O, Ronen G, Nishri Y, Solomon M, Cohen G, Levine A (2006) Suppression of Arabidopsis vesicle-SNARE expression inhibited fusion of H2O2-containing vesicles with tonoplast and increased salt tolerance. Proc Natl Acad Sci USA 103: 18008-18013.
20. Lipka V, Kwon C, Panstruga R (2007) SNARE-ware: the role of SNARE-domain proteins in plant biology. Annu Rev Cell Dev Biol 23: 147-174.
21. Meyer D, Pajonk S, Micali C, O'Connell R, Schulze-Lefert P (2008) Extracellular transport and integration of plant secretory proteins into pathogen-induced cell wall compartments. Plant J 57: 986-999.
22. Muller A, Guan C, Galweiler L, Tanzler P, Huijser P, Marchant A, Parry G, Bennett M, Wisman E, Palme K (1998) AtPIN2 defines a locus of Arabidopsis for root gravitropism control. EMBO J 17: 6903-6911.
23. Niihama M, Uemura T, Saito C, Nakano A, Sato MH, Tasaka M, Morita MT (2005) Conversion of functional specificity in Qb-SNARE VTI1 homologues of Arabidopsis. Curr Biol 15: 555-560.
24. Ohtomo I, Ueda H, Shimada T, Nishiyama C, Komoto Y, Hara-Nishimura I, Takahashi T (2005) Identification of an allele of VAM3/SYP22 that confers a semi-dwarf phenotype in Arabidopsis thaliana. Plant Cell Physiol 46: 1358-1365.
25. Parlati F, Varlamov O, Paz K, McNew JA, Hurtado D, Sollner TH, Rothman JE (2002) Distinct SNARE complexes mediating membrane fusion in Golgi transport based on combinatorial specificity. Proc Natl Acad Sci USA 99: 5424-5429.
26. Paulmurugan R, Massoud TF, Huang J, Gambhir SS (2004) Molecular imaging of drug-modulated protein-protein interactions in living subjects. Cancer Res 64: 2113-2119.
27. Rual JF, Venkatesan K, Hao T, Hirozane-Kishikawa T, Dricot A, Li N, Berriz GF, Gibbons FD, Dreze M, Ayivi-Guedehoussou N, Klitgord N, Simon C, Boxem M, Milstein S, Rosenberg J, Goldberg DS, Zhang LV, Wong SL, Franklin G, Li S, Albala JS, Lim J, Fraughton C, Llamosas E, Cevik S, Bex C, Lamesch P, Sikorski RS, Vandenhaute J, Zoghbi HY, Smolyar A, Bosak S, Sequerra R, Doucette-Stamm L, Cusick ME, Hill DE, Roth FP, Vidal M (2005) Towards a proteome-scale map of the human protein-protein interaction network. Nature 437: 1173-1178.
28. Sanderfoot AA, Kovaleva V, Bassham DC, Raikhel NV (2001) Interactions between syntaxins identify at least five SNARE complexes within the Golgi/prevacuolar system of the Arabidopsis cell. Mol Biol Cell 12: 3733-3743.
29. Sato MH, Nakamura N, Ohsumi Y, Kouchi H, Kondo M, Hara-Nishimura I, Nishimura M, Wada Y (1997) The AtVAM3 encodes a syntaxin-related molecule implicated in the vacuolar assembly in Arabidopsis thaliana. J Biol Chem 272: 24530-24535.
30. Schoch S, Deak F, Konigstorfer A, Mozhayeva M, Sara Y, Sudhof TC, Kavalali ET (2001) SNARE function analyzed in synaptobrevin/VAMP knockout mice. Science 294: 1117-1122.
31. Schuette CG, Hatsuzawa K, Margittai M, Stein A, Riedel D, Kuster P, Konig M, Seidel C, Jahn R (2004) Determinants of liposome fusion mediated by synaptic SNARE proteins. Proc Natl Acad Sci USA 101: 2858-2863.
32. Schulze WX, Reinders A, Ward J, Lalonde S, Frommer WB (2003) Interactions between co-expressed Arabidopsis sucrose transporters in the split-ubiquitin system. BMC Biochem 4: 3.
33. Stefan E, Aquin S, Berger N, Landry CR, Nyfeler B, Bouvier M, Michnick SW (2007) Quantification of dynamic protein complexes using Renilla luciferase fragment complementation applied to protein kinase A activities in vivo. Proc Natl Acad Sci USA 104: 16916-16921.
34. Stein M, Dittgen J, Sanchez-Rodriguez C, Hou BH, Molina A, Schulze-Lefert P, Lipka V, Somerville S (2006) Arabidopsis PEN3/PDR8, an ATP binding cassette transporter, contributes to nonhost resistance to inappropriate pathogens that enter by direct penetration. Plant Cell 18: 731-746.
35. Surpin M, Raikhel N (2004) Traffic jams affect plant development and signal transduction. Nat Rev Mol Cell Biol 5: 100-109.
36. Tanaka H, Kitakura S, De Rycke R, De Groodt R, Friml J (2009) Fluorescence imaging-based screen identifies ARF GEF component of early endosomal trafficking. Curr Biol 19: 391-397.
37. 2+-regulated membrane fusion by synaptotagmin and SNAREs. Science 304: 435-438.
38. Uemura T, Ueda T, Ohniwa RL, Nakano A, Takeyasu K, Sato MH (2004) Systematic analysis of SNARE molecules in Arabidopsis: dissection of the post-Golgi network in plant cells. Cell Struct Funct 29: 49-65.
39. Walter M, Chaban C, Schutze K, Batistic O, Weckermann K, Nake C, Blazevic D, Grefen C, Schumacher K, Oecking C, Harter K, Kudla J (2004) Visualization of protein interactions in living plant cells using bimolecular fluorescence complementation. Plant J 40: 428-438.
40. Weber T, Zemelman BV, McNew JA, Westermann B, Gmachl M, Parlati F, Sollner TH, Rothman JE (1998) SNAREpins: minimal machinery for membrane fusion. Cell 92: 759-772.
41. Wrona AF, Spanswick RM, Aist JR (1988) Calcium transport in protoplasts isolated from ml-o barley isolines resistant and susceptible to powdery mildew. Plant Physiol 88: 1157-1162.
42. Xia Z, Zhou Q, Lin J, Liu Y (2001) Stable SNARE complex prior to evoked synaptic vesicle fusion revealed by fluorescence resonance energy transfer. J Biol Chem 276: 1766-1771.
43. Yano D, Sato M, Saito C, Sato MH, Morita MT, Tasaka M (2003) A SNARE complex containing SGR3/AtVAM3 and ZIG/VTI11 in gravity-sensing cells is important for Arabidopsis shoot gravitropism. Proc Natl Acad Sci USA 100: 8589-8594.
44. Yoshizawa AC, Kawashima S, Okuda S, Fujita M, Itoh M, Moriya Y, Hattori M, Kanehisa M (2006) Extracting sequence motifs and the phylogenetic features of SNARE-dependent membrane traffic. Traffic 7: 1104-1118.