Identification of UV/blue light-response elements in the Arabidopsis thaliana chalcone synthase promoter using a homologous protoplast transient expression system

Plant Molecular Biology

Volumn 36, Issue 5, 1998, Pages 741-754

  Hartmann, Ulrike ^a   Valentine, William J ^b   Christie, John M ^b,c   Hays, John ^a,d   Jenkins, Gareth I ^b   Weisshaar, Bernd ^a  

^a MAX PLANCK INSTITUTE FOR PLANT BREEDING RESEARCH   (Germany)

^b UNIVERSITY OF GLASGOW   (United Kingdom)

^c GEOPHYSICAL LABORATORY   (United States)

^d OREGON STATE UNIVERSITY   (United States)

Author keywords

ACGT containing element; CHS; G box; Promoter structure; UV A blue light; UV B light

Indexed keywords

DELETION MUTAGENESIS; PETROSELINUM CRISPUM; PROMOTER REGION; PROTOPLAST; TRANSCRIPTION REGULATION; ULTRAVIOLET IRRADIATION;

ACYLTRANSFERASES; ARABIDOPSIS; BASE SEQUENCE; DNA, PLANT; GENE EXPRESSION REGULATION, PLANT; GENES, PLANT; LIGHT; MOLECULAR SEQUENCE DATA; MUTAGENESIS; PROMOTER REGIONS (GENETICS); PROTOPLASTS; SEQUENCE DELETION; ULTRAVIOLET RAYS; VARIATION (GENETICS);

ARABIDOPSIS THALIANA; EMBRYOPHYTA; PETROSELINUM CRISPUM;

EID: 0032033377     PISSN: 01674412     EISSN: None     Source Type: Journal    
DOI: 10.1023/A:1005921914384     Document Type: Article

References (58)

1. Abel S, Theologis A: Transient transformation of Arabidopsis leaf protoplasts: a versatile experimental system to study gene expression. Plant J 5: 421-427 (1994).
2. Ahmad M, Cashmore AR: HY4 gene of A.thaliana encodes a protein with characteristics of a blue-light photoreceptor. Nature 366: 162-166 (1993).
3. Ahmad M, Cashmore AR: Seeing blue: the discovery of cryptochrome. Plant Mol Biol 30: 851-861 (1996).
4. Armstrong GA, Weisshaar B, Hahlbrock K: Homodimeric and heterodimeric leucine zipper proteins and nuclear factors from parsley recognize diverse promoter elements with ACGT cores. Plant Cell 4: 525-537 (1992).
5. Axelos M, Curie C, Mazzolini L, Bardet C, Lescure B: A protocol for transient gene expression in Arabidopsis thaliana protoplasts isolated from cell suspension cultures. Plant Physiol Biochem 30: 123-128 (1992).
6. Batschauer A, Ehmann B, Schäfer E: Cloning and characterization of a chalcone synthase gene from mustard and its light-dependent expression. Plant Mol Biol 16: 175-185 (1991).
7. Batschauer A, Gilmartin PM, Nagy F, Schäfer E: The molecular biology of photoregulated genes. In: Kendrick RE, Kronenberg GHM (ed) Photomorphogenesis in Plants, 2nd ed., pp. 559-599. Kluwer Academic Publishers, Dordrecht, Netherlands (1994).
8. Batschauer A, Rocholl M, Kaiser T, Nagatani A, Furuya M, Schäfer E: Blue and UV-A light-regulated CHS expression in Arabidopsis independent of phytochrome A and phytochrome B. Plant J 9: 63-69 (1996).
9. Blecken J, Weisshaar B, Herzfeld F: Two distinct cis-acting elements are involved in light-dependent activation of the pea clip promoter. Mol Gen Genet 245: 371-379 (1994).
10. Block A, Dangl JL, Hahlbrock K, Schulze-Lefert P: Functional borders, genetic fine structure, and distance requirements of cis elements mediating light responsiveness of the parsley chalcone synthase promoter. Proc Natl Acad Sci USA 87: 5387-5391 (1990).
11. Büttner M, Singh KB: Arabidopsis thaliana ethylene-responsive element binding protein (AtEBP), an ethylene-inducible, GCC box DNA-binding protein interacts with an ocs element binding protein. Proc Natl Acad Sci USA 94: 5961-5966 (1997).
12. Christie JM, Jenkins GI: Distinct UV-B and UV-A/blue light signal transduction pathways induce chalcone synthase gene expression in Arabidopsis cells. Plant Cell 8: 1555-1567 (1996).
13. Cooke R et al.: Further progress towards a catalogue of all Arabidopsis genes: analysis of a set of 5000 non-redundant ESTs. Plant J 9: 101-124 (1996).
14. Dangl JL, Hahlbrock K, Schell J: Regulation and stucture of chalcone synthase genes. In: Vasil JK, Schell J (ed) Cell Culture and Somatic Cell Genetics of Plants, vol. 6, pp. 155-173. Academic Press, New York (1989).
15. Dangl JL, Hauffe K-D, Lipphardt S, Hahlbrock K, Scheel D: Parsley protoplasts retain differential responsiveness to UV light and fungal elicitor. EMBO J 6: 2551-2556 (1987).
16. Faktor O, Loake G, Dixon RA, Lamb CJ: The G-box and H-box in a 39 bp region of a French bean chalcone synthase promoter constitute a tissue-specific regulatory element. Plant J 11: 1105-1113 (1997).
17. Feinbaum RL, Ausubel FM: Transcriptional regulation of the Arabidopsis thaliana chalcone synthase gene. Mol Cell Biol 8: 1985-1992 (1988).
18. Feinbaum RL, Storz G, Ausubel FM: High intesity and blue light regulated expression of chimeric chalcone synthase genes in transgenic Arabidopsis thaliana plants. Mol Gen Genet 226: 449-456 (1991).
19. Feinberg AP, Vogelstein B: A technique for radiolabeling DNA restriction endonuclease fragments to high specific activity. Anal Biochem 137: 266-267 (1984).
20. Feldbrügge M, Hahlbrock K, Weisshaar B: The transcriptional regulator CPRF1: expression analysis and gene structure. Mol Gen Genet 251: 619-627 (1996).
21. Feldbrügge M, Sprenger M, Dinkelbach M, Yazaki K, Harter K, Weisshaar B: Functional analysis of a light-responsive plant bZIP transcriptional regulator. Plant Cell 6: 1607-1621 (1994).
22. Feldbrügge M, Sprenger M, Hahlbrock K, Weisshaar B: PcMYB1, a novel plant protein containing a DNA-binding domain with one MYB repeat, interacts in vivo with a light-regulatory promoter unit. Plant J 11: 1079-1093 (1997).
23. Foster R, Izawa T, Chua N-H: Plant bZIP proteins gather at ACGT elements. FASEB J 8: 192-200 (1994).
24. Fuglevand G, Jackson JA, Jenkins GI: UV-B, UV-A, and blue light signal transduction pathways interact synergistically to regulate chalcone synthase gene expression in Arabidopsis. Plant Cell 8: 2347-2357 (1996).
25. Giuliano G, Pichersky E, Malik VS, Timko MP, Scolnic PA, Cashmore AR: An evolutionary conserved protein binding sequence upstream of a plant light-regulated gene. Proc Natl Acad Sci USA 85: 7089-7093 (1988).
26. Izawa T, Foster R, Chua NH: Plant bZIP protein DNA binding specificity. J Mol Biol 230: 1131-1144 (1993).
27. Jackson JA, Fuglevand G, Brown BA, Shaw MJ, Jenkins GI: Isolation of Arabidopsis mutants altered in the light-regulation of chalcone synthase gene expression using a transgenic screening approach. Plant J 8: 369-380 (1995).
28. Jackson JA, Jenkins GI: Extension-growth and expression of flavonoid biosynthesis genes in the Arabidopsis hy4 mutant. Planta 197: 233-239 (1995).
29. Jefferson RJ: Assaying chimaeric genes in plants: The GUS gene fusion system. Plant Mol Biol Rep 5: 387-405 (1987).
30. Jenkins GI: UV and blue light signal transduction in Arabidopsis. Plant Cell Envir 20: 773-778 (1997).
31. Jenkins GI, Christie JM, Fuglevand G, Long JC, Jackson JA: Plant responses to UV and blue light: biochemical and genetic approaches. Plant Sci 112: 117-138 (1995).
32. Kaiser T, Batschauer A: Cis-acting elements of the CHS1 gene from white mustard controlling promoter activity and spatial patterns of expression. Plant Mol Biol 28: 231-243 (1995).
33. Kaiser T, Emmler K, Kretsch T, Weisshaar B, Schäfer E, Batschauer A: Promoter elements of the mustard CHS1 gene sufficient for light regulation in transgenic plants. Plant Mol Biol 28: 219-229 (1995).
34. Kaufman LS: Transduction of blue-light signals. Plant Physiol 102: 333-337 (1993).
35. Kawalleck P, Somssich IE, Feldbrügge M, Hahlbrock K, Weisshaar B: Polyubiquitin gene expression and structural properties of the ubi4-2 gene in Petroselinum crispum. Plant Mol Biol 21: 673-684 (1993).
36. Kombrink E, Hahlbrock K: Responses of cultured parsley cells to elicitors from phytopathogenic fungi. Plant Physiol 81: 216-221 (1986).
37. Koornneef M, Rolff E, Spruit CJP: Genetic control of light-inhibited hypocotyl elongation in Arabidopsis thaliana (L.) Heynh. Pflanzenphysiol 100: 147-160 (1980).
38. Korfhage U, Trezzini GF, Meier I, Hahlbrock K, Somssich IE: Plant homeodomain protein involved in transcriptional regulation of a pathogen defense-related gene. Plant Cell 6: 695-708 (1994).
39. Kubasek WL, Shirley BW, McKillop A, Goodman HM, Briggs W, Ausubel FM: Regulation of flavonoid biosynthetic genes in germinating Arabidopsis seedlings. Plant Cell 4: 1229-1236 (1992).
40. Landt O, Grunert H-P, Hahn U: A general method for rapid site-directed mutagenesis using the polymerase chain reaction. Gene 96: 125-128 (1990).
41. Lubberstedt T, Bolle CE, Sopory S, Flieger K, Herrmann RG, Oelmuller R: Promoters from genes for plastid proteins possess regions with different sensitivities toward red and blue light. Plant Physiol 104: 997-1006 (1994).
42. Luehrsen KR, de Wet JR, Walbot V: Transient expression analysis in plants using firefly luciferase reporter gene. Meth Enzymol 216: 397-414 (1992).
43. Marrs KA, Kaufman LS: Blue-light regulation of transcription for nuclear genes in pea. Proc Natl Acad Sci USA 86: 4492-4495 (1989).
44. Martin C, Paz-Ares J: MYB transcription factors in plants. Trends Genet 13: 67-73 (1997).
45. May MJ, Leaver CJ: Oxidative stimulation of glutathione synthesis in Arabidopsis thaliana suspension cultures. Plant Physiol 103: 621-627 (1993).
46. Menkens AE, Schindler U, Cashmore AR: The G-box: a ubiquitous regulatory DNA element in plants bound by the GBF family of bZIP proteins. Trends Biochem Sci 20: 506-510 (1995).
47. Rocholl M, Talke-Messerer C, Kaiser T, Batschauer A: Unit1 of the mustard chalcone synthase promoter is sufficient to mediate light responses from different photoreceptors. Plant Sci 97: 189-198 (1994).
48. Sambrook J, Fritsch EF, Maniatis T: Molecular Cloning: A Laboratory Manual, 2nd ed. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY (1989).
49. Sarkar G, Sommer SS: The 'megaprimer' method of site-directed mutagenesis. BioTechniques 8: 404-407 (1990).
50. Sawbridge TI, Lopez-Juez E, Knight MR, Jenkins GI: A blue-light photoreceptor mediates the fluence-rate-dependent expression of genes encoding the small subunit of ribulose 1,5-bisphosphate carboxylase/oxygenase in light-grown Phaseolus vulgaris primary leaves. Planta 192: 1-8 (1994).
51. Schulze-Lefert P, Becker-Andre M, Schulz W, Hahlbrock K, Dangl JL: Functional architecture of the light-responsive chalcone synthase promoter from parsley. Plant Cell 1: 707-714 (1989).
52. Schulze-Lefert P, Dangl JL, Becker-André M, Hahlbrock K, Schulz W: Inducible in vivo DNA footprints define sequences necessary for UV light activation of the parsley chalcone synthase gene. EMBO J 8: 651-656 (1989).
53. Short TW, Briggs WR: The transduction of blue light signals in higher plants. Annu Rev Plant Physiol Plant Mol Biol 45: 143-171 (1994).
54. Tovar Torres J, Block A, Hahlbrock K, Somssich IE: Influence of bacterial strain genotype on transient expression of plasmid DNA in plant protoplasts. Plant J 4: 587-592 (1993).
55. Trezzini GF, Horrichs A, Somssich IE: Isolation of putative defense-related genes from Arabidopsis thaliana and expression in fungal elicitor-treated cells. Plant Mol Biol 21: 385-389 (1993).
56. Weisshaar B, Armstrong GA, Block A, da Costa e Silva O, Hahlbrock K: Light-inducible and constitutively expressed DNA-binding proteins recognizing a plant promoter element with functional relevance in light responsiveness. EMBO J 10: 1777-1786 (1991).
57. Weisshaar B, Block A, Armstrong GA, Herrmann A, Schulze-Lefert P, Hahlbrock K: Regulatory elements required for light-mediated expression of the Petroselinum crispum chalcone synthase gene. In: Jenkins GI, Schuch W (ed) Molecular Biology of plant Development, pp. 191-210. The Company of Biologists, Cambridge, UK (1991).
58. Williams ME, Foster R, Chua N-H: Sequences flanking the hexameric G-box core CACGTG affect the specificity of protein binding. Plant Cell 4: 485-496 (1992).