Random primed gene walking PCR: A simple procedure to retrieve nucleotide fragments adjacent to known DNA sequences

BioTechniques

Volumn 21, Issue 1, 1996, Pages 20-

  Trueba, Gabriel A ^a   Johnson, Russell C ^a  

^a UNIVERSITY OF MINNESOTA MEDICAL SCHOOL   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

DNA FRAGMENT;

ARTICLE; DNA SEQUENCE; HUMAN; MOLECULAR CLONING; NUCLEOTIDE SEQUENCE; POLYMERASE CHAIN REACTION;

EID: 8944252803     PISSN: 07366205     EISSN: None     Source Type: Journal    
DOI: 10.2144/96211bm02     Document Type: Article

References (5)

1. Dominguez, O. and C. Lopez-Larrea. 1994. Gene walking by unpredictably primed PCR. Nucleic Acids Res. 22:3247-3248.
2. Malo, M.S., K. Srivastava, J.M. Andresen, X.N. Chen, J.R. Korenberg and V.M. Ingram. 1994. Targeted gene walking by low stringency polymerase chain reaction: assignment of a putative human brain sodium channel gene (SCN3A) to chromosome 2q24-31. Proc. Natl. Acad. Sci. USA 97:2975-2979.
3. Parker, J.D., P.S. Rabinovitch and G.C. Burmer. 1991. Targeted gene walking polymerase chain reaction. Nucleic Acids Res. 19:3055-3060.
4. Sambrook, J., E.F. Fritsch and T. Maniatis. 1989. Molecular Cloning: A Laboratory Manual. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY.
5. Triglia, T., M.G. Peterson and D.J. Kemp. 1988. A procedure for in vitro amplification of DNA segments that lie outside the boundaries of known sequences. Nucleic Acids Res. 16:8186.