Laser microdissection of immunolabeled astrocytes allows quantification of astrocytic gene expression

Journal of Neuroscience Methods

Volumn 138, Issue 1-2, 2004, Pages 141-148

  Burbach, Guido J ^a   Dehn, Doris ^a   Nagel, Barbara ^a   Del Turco, Domenico ^a   Deller, Thomas ^a  

^a GOETHE UNIVERSITY   (Germany)

Author keywords

Brain injury; Entorhinal cortex lesion; Gene profiling; Glia; Glial fibrillary acidic protein; Quantitative RT PCR

Indexed keywords

GLIAL FIBRILLARY ACIDIC PROTEIN; MESSENGER RNA; RNA 18S; RNA 28S;

ANIMAL CELL; ANIMAL EXPERIMENT; ANIMAL MODEL; ANIMAL TISSUE; ANTIBODY LABELING; ARTICLE; ASTROCYTE; BRAIN CORTEX LESION; CELL SPECIFICITY; CELL TYPE; CLINICAL PROTOCOL; CONTROLLED STUDY; ENTORHINAL CORTEX; GENE EXPRESSION; GENE EXPRESSION PROFILING; IMMUNOHISTOCHEMISTRY; INSTRUMENTATION; INTERMETHOD COMPARISON; LASER MICRODISSECTION; MALE; MOUSE; NONHUMAN; PRIORITY JOURNAL; PROTEIN CONTENT; PROTEIN EXPRESSION; PROTEIN LOCALIZATION; QUANTITATIVE ANALYSIS; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; ANIMAL; BIOSYNTHESIS; C57BL MOUSE; COMPARATIVE STUDY; GENETICS; HEMISPHERIC DOMINANCE; LASER; METABOLISM; METHODOLOGY; MICRODISSECTION; NONPARAMETRIC TEST; UPREGULATION;

ANIMALS; ASTROCYTES; FUNCTIONAL LATERALITY; GENE EXPRESSION; GLIAL FIBRILLARY ACIDIC PROTEIN; IMMUNOHISTOCHEMISTRY; LASERS; MALE; MICE; MICE, INBRED C57BL; MICRODISSECTION; REVERSE TRANSCRIPTASE POLYMERASE CHAIN REACTION; RNA, MESSENGER; RNA, RIBOSOMAL, 18S; RNA, RIBOSOMAL, 28S; STATISTICS, NONPARAMETRIC; UP-REGULATION;

EID: 7744232930     PISSN: 01650270     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.jneumeth.2004.03.022     Document Type: Article

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