Evaluation of a real-time polymerase chain reaction assay for the laboratory diagnosis of giardiasis

Diagnostic Microbiology and Infectious Disease

Volumn 66, Issue 3, 2010, Pages 261-267

  Calderaro, Adriana ^a   Gorrini, Chiara ^a   Montecchini, Sara ^a   Peruzzi, Simona ^a   Piccolo, Giovanna ^a   Rossi, Sabina ^a   Gargiulo, Franco ^b   Manca, Nino ^b   Dettori, Giuseppe ^a   Chezzi, Carlo ^a  

^a UNIVERSITY HOSPITAL OF PARMA   (Italy)

^b UNIVERSITY OF BRESCIA   (Italy)

Author keywords

Genotyping; Giardia intestinalis; Real time PCR

Indexed keywords

PROTOZOAL DNA;

ADULT; ARTICLE; CHILD; CONTROLLED STUDY; DIAGNOSTIC ACCURACY; DIAGNOSTIC PROCEDURE; DNA EXTRACTION; FECES ANALYSIS; GENOTYPE; GIARDIA LAMBLIA; GIARDIASIS; HUMAN; INTERMETHOD COMPARISON; LABORATORY DIAGNOSIS; MAJOR CLINICAL STUDY; MICROSCOPY; NONHUMAN; PRIORITY JOURNAL; REAL TIME POLYMERASE CHAIN REACTION; SENSITIVITY AND SPECIFICITY;

ADULT; BACTERIAL TYPING TECHNIQUES; CHILD; CYTOSKELETAL PROTEINS; FECES; GIARDIA LAMBLIA; GIARDIASIS; HUMANS; POLYMERASE CHAIN REACTION; POLYMORPHISM, RESTRICTION FRAGMENT LENGTH; PROTOZOAN PROTEINS; SENSITIVITY AND SPECIFICITY;

EID: 77349110195     PISSN: 07328893     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.diagmicrobio.2009.10.004     Document Type: Article

References (26)

1. Cacciò S.M., De Giacomo M., Pozio E. Sequence analysis of the β-giardin gene and development of a polymerase chain reaction-restriction fragment length polymorphism assay to genotype Giardia duodenalis cysts from human faecal samples. Int. J. Parasitol. 2002, 32:1023-1030.
2. Cacciò S.M., Thompson R.C.A., McLauchlin J., Smith H. Unravelling Cryptosporidium and Giardia epidemiology. Trends Parasitol. 2005, 21:430-437.
3. Cacciò S.M., Ryan U. Molecular epidemiology of giardiasis. Mol. Biochem. Parasitol. 2008, 160:75-80.
4. Cacciò S.M., Beck R., Lalle M., Marinculic A., Pozio E. Multilocus genotyping of Giardia duodenalis reveals striking differences between assemblages A and B. Int. J. Parasitol. 2008, 38:1523-1531.
5. Calderaro A., Gorrini C., Bommezzadri S., Piccolo G., Dettori G., Chezzi C. Entamoeba histolytica and Entamoeba dispar: comparison of two PCR assay for diagnosis in a non-endemic setting. Trans. Roy. Soc. Trop. Med. Hyg. 2006, 100:450-457.
6. de Lalla F., Rinaldi E., Santoro D., Nicolin R., Tramarin A. Outbreak of Entamoeba histolytica and Giardia lamblia infections in travellers returning from the tropics. Infection 1992, 20:78-82.
7. Dupont H., Sullivan P.S. Giardiasis: the clinical spectrum, diagnosis and therapy. Pediatr Infect Dis 1986, 5:131-138.
8. Faubert G. Immune response to Giardia duodenalis. Clin. Microbiol. Rev. 2000, 13:25-54.
9. Garcia L.S., Bruckner D.A. Giardia lamblia key points-laboratory diagnosis. Diagnostic medical parasitology 1997, 3:42-44. ASM PRESS, Washington, DC.
10. Garcia L.S., Shimizu R.Y. Evaluation of nine immunoassay kits (enzyme immunoassay and direct fluorescence) for detection of Giardia lamblia and Cryptosporidium parvum in human fecal specimens. J. Clin. Microbiol. 1997, 35:1526-1529.
11. Gelanew T., Lalle M., Hailu A., Pozio E., Cacciò S.M. Molecular characterization of human isolates of Giardia duodenalis from Ethiopia. Acta Trop. 2007, 102:92-99.
12. Giangaspero A., Berrilli F., Brandonisio O. Giardia and Cryptosporidium and public health: the epidemiological scenario from the Italian perspective. Parasitol. Res. 2007, 101:1169-1182.
13. Haque R., Roy S., Kabir M., Stroup S.E., Mondal D., Houpt E.R. Giardia assemblage A infection and diarrhea in Bangladesh. J. Infect. Dis. 2005, 192:2171-2173.
14. Haque R., Roy S., Siddique A., Mondal U., Rahman S.M.M., Mondal D., Houpt E., Petri W. Multiplex real-time PCR assay for detection of Entamoeba histolytica, Giardia intestinalis and Cryptosporidium spp. Am. J. Trop. Med. Hyg. 2007, 76:713-717.
15. Homan W.L., Mank T.G. Human giardiasis: genotype linked differences in clinical symptomatology. Int. J. Parasitol. 2001, 31:822-826.
16. Lalle M., Jimenez-Cardosa E., Cacciò S.M., Pozio E. Genotyping of Giardia duodenalis from humans and dogs from Mexico using a β-giardin nested-polymerase chain reaction assay. J. Parasitol. 2005, 91:203-205.
17. Lalle M., Pozio E., Capelli G., Bruschi F., Crotti D., Cacciò S.M. Genetic heterogeneity at the β-giardin locus among human and animal isolates of Giardia duodenalis and identification of potentially zoonotic subgenotypes. Int. J. Parasitol. 2005, 35:207-213.
18. Peruzzi S., Gorrini C., Piccolo G., Calderaro A., Dettori G., Chezzi C. Prevalence of intestinal parasites in the area of Parma during the year 2005. Acta Biomed. 2006, 77:147-151.
19. Read C., Walters J., Robertson I.D., Thompson R.C.A. Correlation between genotype of Giardia duodenalis and diarrhoea. Int. J. Parasitol. 2002, 32:229-231.
20. Sahagún J., Clavel A., Goñi P., Seral C., Llorente M.T., Castillo F.J., Arias A., Gómes-Lus R. Correlation between the presence of symptoms and the Giardia duodenalis genotype. Eur. J. Clin. Microbiol. Infect. Dis. 2008, 27:81-83.
21. Schuurman T., Lankamp P., van Belkum A., Kooistra-Smid M., van Zwet A. Comparison of microscopy, real-time PCR and a rapid immunoassay for the detection of Giardia lamblia in human stool specimens. Clin. Microbiol. Infect. 2007, 13:1186-1191.
22. Sloan L.M. Real-time PCR in clinical microbiology: verification, validation and contamination control. Clin. Microbiol. Newslett. 2007, 29:87-95.
23. Smith H.V., Cacciò S.M., Cook N., Nichols R.A.B., Tait A. Cryptosporidium and Giardia as foodborne zoonoses. Vet. Parasitol. 2007, 149:29-40.
24. Thompson R.C.A., Hopkins R.M., Homan W.L. Nomenclature and genetic groupings of Giardia infecting mammals. Parasitol. Today 2000, 16:210-213.
25. Verweij J.J., Schinkel J., Laeijendecker D., van Rooyen M.A.A., van Lieshout L., Polderman A.M. Real-time PCR for the detection of Giardia lamblia. Mol. Cell. Probes 2003, 17:223-225.
26. Verweij J.J., Blangé R.A., Templeton K., Schinkel J., Brienen E.A.T., van Rooyen M.A.A., van Lieshout L., Polderman A.M. Simultaneous detection of Entamoeba histolytica, Giardia intestinalis and Cryptosporidium parvum in fecal samples by using multiplex real-time PCR. J. Clin. Microbiol. 2004, 42:1220-1223.