Entamoeba histolytica and Entamoeba dispar: Comparison of two PCR assays for diagnosis in a non-endemic setting

Transactions of the Royal Society of Tropical Medicine and Hygiene

Volumn 100, Issue 5, 2006, Pages 450-457

  Calderaro, Adriana ^a   Gorrini, Chiara ^a   Bommezzadri, Simona ^a   Piccolo, Giovanna ^a   Dettori, Giuseppe ^a   Chezzi, Carlo ^a  

^a UNIVERSITY OF PARMA   (Italy)

Author keywords

Amoebiasis; Conventional PCR; Entamoeba dispar; Entamoeba histolytica; Epidemiology; Real time PCR

Indexed keywords

PROTOZOAL DNA;

ADULT; AMEBIASIS; ARTICLE; CHILD; CONTROLLED STUDY; CULTURE TECHNIQUE; DIAGNOSTIC ACCURACY; DIAGNOSTIC PROCEDURE; DNA SEQUENCE; ENTAMOEBA DISPAR; ENTAMOEBA HISTOLYTICA; FECES ANALYSIS; FEMALE; HUMAN; HUMAN CELL; HUMAN TISSUE; INTERMETHOD COMPARISON; INTESTINE BIOPSY; LIVER ABSCESS; LIVER BIOPSY; MAJOR CLINICAL STUDY; MALE; MICROSCOPY; NUCLEOTIDE SEQUENCE; PARASITE IDENTIFICATION; POLYMERASE CHAIN REACTION; REAL TIME POLYMERASE CHAIN REACTION; SENSITIVITY AND SPECIFICITY; SEQUENCE ANALYSIS; SEROLOGY; STRAIN DIFFERENCE;

ANIMALS; BASE SEQUENCE; DIAGNOSIS, DIFFERENTIAL; DNA PRIMERS; DNA, PROTOZOAN; DYSENTERY, AMEBIC; ENTAMOEBA; ENTAMOEBA HISTOLYTICA; ENTAMOEBIASIS; FECES; FEMALE; HUMANS; ITALY; LIVER ABSCESS, AMEBIC; MALE; MOLECULAR SEQUENCE DATA; POLYMERASE CHAIN REACTION; REVERSE TRANSCRIPTASE POLYMERASE CHAIN REACTION; SENSITIVITY AND SPECIFICITY;

ENTAMOEBA DISPAR; ENTAMOEBA HISTOLYTICA;

EID: 33644841546     PISSN: 00359203     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.trstmh.2005.07.015     Document Type: Article

References (14)

1. J. Blessmann H. Buss P.A. Ton Nu B.T. Dinh Q.T. Viet Ngo A. Le Van M.D. Abd Alla T.F.G.H. Jackson J.I. Ravdin E. Tannich Real-time PCR for detection and differentiation of Entamoeba histolytica and Entamoeba dispar in fecal samples J. Clin. Microbiol. 40 2002 4413-4417
2. L.S. Diamond C.G. Clark A redescription of Entamoeba histolytica Schaudinn, 1903 (emended Walker, 1911), separating it from Entamoeba dispar Brumpt, 1925 J. Eukaryot. Microbiol. 40 1903 340-344
3. S. Gatti A.M. Bernuzzi R. Maserati M. Scaglia Incidence of Entamoeba histolytica/Entamoeba dispar in international travellers, extracommunitary immigrants, and adopted children Arch. Med. Res. 31 2000 S47-S48
4. P. Gonin L. Trudel Detection and differentiation of Entamoeba histolytica and Entamoeba dispar isolates in clinical samples by PCR and enzyme-linked immunosorbent assay J. Clin. Microbiol. 41 2003 237-241
5. R. Haque C.D. Huston M. Hughes E. Houpt W.A. Petri Jr Amoebiasis. N. Engl. J. Med. 348 2003 1565-1573
6. S. Katzwinkel-Wladarsch T. Loscher H. Rinder Direct amplification and differentiation of pathogenic and nonpathogenic Entamoeba histolytica DNA from stool specimens Am. J. Trop. Med. Hyg. 51 1994 115-118
7. A. Kebede J.J. Verweij T. Endeshaw T. Messele G. Tasew B. Petros A.M. Polderman The use of real-time PCR to identify Entamoeba histolytica and E. dispar infections in prisoners and primary-school children in Ethiopia Ann. Trop. Med. Parasitol. 98 2004 43-48
8. C.A. Kreader Relief of amplification inhibition in PCR with bovine serum albumin or T4 gene 32 protein Appl. Environ. Microbiol. 62 1996 1102-1106
9. Y.O. Nunez M.A. Fernandez D. Torres-Nunez J.A. Silva I. Montano J.L. Maestre L. Fonte Multiplex polymerase chain reaction amplification and differentiation of Entamoeba histolytica and Entamoeba dispar DNA from stool samples Am. J. Trop. Med. Hyg. 64 2001 293-297
10. L.S. Ritchie An ether sedimentation technique for the routine stool examination Bull. US Army Med. Dept. 8 1948 326
11. M. Tanyuksel W.A. Petri Jr Laboratory diagnosis of amoebiasis Clin. Microbiol. Rev. 16 2003 713-729
12. J.J. Verweij R.A. Blangé K. Templeton J. Schinkel E.A.T. Brienen M.A.A. van Rooyen L. van Lieshout A.M. Polderman Simultaneous detection of Entamoeba histolytica, Giardia lamblia, and Cryptosporidium parvum in fecal samples by using multiplex real-time PCR J. Clin. Microbiol. 42 2004 1220-1223
13. WHO Amoebiasis Wkly. Epidemiol. Rec. 72 1997 97-100
14. S. Zindrou E. Orozco E. Linder A. Téllez A. Björkman Specific detection of Entamoeba histolytica DNA by hemolysin gene targeted PCR Acta. Trop. 78 2001 117-125