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Volumn 132, Issue 6, 2010, Pages 2005-2015

Reaction mechanisms of the multicopper oxidase CueO from Escherichia coli support its functional role as a cuprous oxidase

Author keywords

[No Author keywords available]

Indexed keywords

AEROBIC CONDITION; AIR-STABLE; BICINCHONINATE; BINDING PROTEINS; CHROMOPHORIC SUBSTRATES; COPPER ATOMS; COPPER REDUCTION; COPPER TOLERANCE; DIOXYGEN REDUCTION; ELECTRON TRANSFER; FREE LIGANDS; MICHAELIS-MENTEN KINETIC; MODEL SUBSTRATES; MULTICOPPER OXIDASE; NEGATIVE SHIFT; NEW APPROACHES; OXIDASE ACTIVITY; OXIDASE ENZYMES; PH BUFFERS; PHENOL OXIDATION; REACTION MECHANISM; STEADY-STATE KINETICS; SUBSTRATE SPECIFICITY; TWO-COMPONENT;

EID: 77249131784     PISSN: 00027863     EISSN: 15205126     Source Type: Journal    
DOI: 10.1021/ja9091903     Document Type: Article
Times cited : (89)

References (45)
  • 18
    • 77249112735 scopus 로고    scopus 로고
    • Martell, A. E.; Smith, R. M.; Motekaitis, R. J. NIST Critically Selected Stability Constants of Metal Complexes, Version 8.0; NIST Standard Reference Database 46; U.S. Department of Commerce, NIST Program: Gaithersburg, MD, 2004; http://www.nist.gov/srd/nist46.htm.
    • Martell, A. E.; Smith, R. M.; Motekaitis, R. J. NIST Critically Selected Stability Constants of Metal Complexes, Version 8.0; NIST Standard Reference Database 46; U.S. Department of Commerce, NIST Program: Gaithersburg, MD, 2004; http://www.nist.gov/srd/nist46.htm.
  • 23
    • 34247869641 scopus 로고    scopus 로고
    • A smaller β2, 4.6 × 1014 M-2 for [CuI(Bca)2]3- was estimated independently by isothermal titration calorimetry: Yatsunyk, L. A, Rosenzweig, A. C. J. Biol. Chem. 2007, 282, 8622-8631. In that work, the Cu+ solution was generated by reduction of Cu2+ with ascorbate in 200 mM NaCl, which stabilizes Cu+ as [CuICl4] 3- and related chloro complexes. Titration with Bca would involve a conversion of species such as [CuICl4]3, rather than Cu+ aq) to [CuI(Bca) 2]3, diminishing the observed heat release
    • 3-, diminishing the observed heat release.
  • 24
    • 77249123350 scopus 로고    scopus 로고
    • The free Cu+ concentration buffered by Bca and Bcs may be calculated by the formula [Cu, CuIL 2, L]2(β2)-1 and thus is dependent on both concentrations of [CuIL2] and [L, Assuming that 50% of ligand L binds CuI and 50% is free, i.e, L, 2[CuIL2, then [Cu, CuIL 2, 2[CuIL2])2)(β2, 1, 1/4, CuIL2, 1(β2)-1. As a metal buffer, Cu IL2] concentrations are usually controlled in the range 10-50 μM, correlating to [Cu, concentrations in the range 1.6 × 10-13-3.2 × 10-14 M for L, Bca and 4.0 × 10-16-7.9 × 10-17 M for L, Bcs, according to respective β2 values reported
    • +].


* 이 정보는 Elsevier사의 SCOPUS DB에서 KISTI가 분석하여 추출한 것입니다.