Single ovalbumin molecules exploring nucleoplasm and nucleoli of living cell nuclei

Biochimica et Biophysica Acta - Molecular Cell Research

Volumn 1803, Issue 3, 2010, Pages 396-404

  Speil, Jasmin ^a   Kubitscheck, Ulrich ^a  

^a UNIVERSITY OF BONN   (Germany)

Author keywords

Diffusion; Fluorescence microscopy; Molecular mobility; Nucleolus; Nucleus; Single molecule microscopy

Indexed keywords

OVALBUMIN;

ARTICLE; CELL NUCLEOPLASM; CELL STRUCTURE; CONFOCAL LASER MICROSCOPY; CONTROLLED STUDY; CYTOPLASM; DIFFUSION; FLUORESCENCE MICROSCOPY; HELA CELL; HUMAN; HUMAN CELL; HUMAN CELL CULTURE; IMAGE PROCESSING; NUCLEOLUS; PRIORITY JOURNAL; VISCOSITY;

ANIMALS; CELL NUCLEOLUS; CELL NUCLEUS; FLUORESCENT DYES; HELA CELLS; HUMANS; MICROSCOPY, CONFOCAL; OVALBUMIN; PROTEIN BINDING; RECOMBINANT FUSION PROTEINS;

EID: 77249127365     PISSN: 01674889     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.bbamcr.2009.10.010     Document Type: Article

References (22)

1. Misteli T. Physiological importance of RNA and protein mobility in the cell nucleus. Histochem. Cell Biol. 129 (2008) 5-11
2. Dross N., Spriet C., Zwerger M., Muller G., Waldeck W., and Langowski J. Mapping eGFP oligomer mobility in living cell nuclei. PloS One 4 (2009) e5041
3. Wachsmuth M., Weidemann T., Muller G., Hoffmann-Rohrer U.W., Knoch T.A., Waldeck W., and Langowski J. Analyzing intracellular binding and diffusion with continuous fluorescence photobleaching. Biophys. J. 84 (2003) 3353-3363
4. Handwerger K.E., and Gall J.G. Subnuclear organelles: new insights into form and function. Trends Cell. Biol. 16 (2006) 19-26
5. Seksek O., Biwersi J., and Verkman A.S. Translational diffusion of macromolecule-sized solutes in cytoplasm and nucleus. J. Cell. Biol. 138 (1997) 131-142
6. Gorisch S.M., Richter K., Scheuermann M.O., Herrmann H., and Lichter P. Diffusion limited compartmentalization of mammalian cell nuclei assessed by microinjected macromolecules. Exp. Cell. Res. 289 (2003) 282-294
7. Handwerger K.E., Cordero J.A., and Gall J.G. Cajal bodies, nucleoli, and speckles in the Xenopus oocyte nucleus have a low-density, sponge-like structure. Mol. Biol. Cell 16 (2005) 202-211
8. Grunwald D., Martin R.M., Buschmann V., Bazett-Jones D.P., Leonhardt H., Kubitscheck U., and Cardoso M.C. Probing intranuclear environments at the single molecule level. Biophys. J. 94 (2008) 2847-2858
9. Siebrasse J.P., Grunwald D., and Kubitscheck U. Single-molecule tracking in eukaryotic cell nuclei. Anal. Bioanal. Chem. 387 (2007) 41-44
10. Siebrasse J.P., and Kubitscheck U. Single molecule tracking for studying nucleocytoplasmic transport and intranuclear dynamics. Methods Mol. Biol. 464 (2009) 343-361
11. Hernandez-Verdun D. Nucleolus: from structure to dynamics. Histochem. Cell Biol. 125 (2006) 127-137
12. Clegg J.S. Properties and metabolism of the aqueous cytoplasm and its boundaries. Am. J. Physiol. 246 (1984) R133-151
13. Siebrasse J.P., and Peters R. Rapid translocation of NTF2 through the nuclear pore of isolated nuclei and nuclear envelopes. EMBO Rep. 3 (2002) 887-892
14. Siebrasse J.-P.a.U.K. Single substrate and transport receptor molecules at the nuclear pore complex. In: Kehlenbach R. (Ed). Nuclear. Transport (2009), Wiley-VCH Weinheim, Germany
15. Anderson C.M., Georgiou G.N., Morrison I.E., Stevenson G.V., and Cherry R.J. Tracking of cell surface receptors by fluorescence digital imaging microscopy using a charge-coupled device camera. Low-density lipoprotein and influenza virus receptor mobility at 4 degrees C. J. Cell. Sci. 101 Pt 2 (1992) 415-425
16. Crank J. The Mathematics of Diffusion,. 2 ed. (1975), Clarendon Press, Oxford
17. Kues T., Dickmanns A., Luhrmann R., Peters R., and Kubitscheck U. High intranuclear mobility and dynamic clustering of the splicing factor U1 snRNP observed by single particle tracking. Proc. Natl. Acad. Sci. U. S. A. 98 (2001) 12021-12026
18. Martin R.M., Tünnemann G., Leonhardt H., and Cardoso M.C. Nucleolar marker for living cells. Histochem. Cell Biol. (2007) 243-251
19. Martin R.M., Leonhardt H., and Cardoso M.C. DNA labeling in living cells. Cytometry A 67 (2005) 45-52
20. Wojcik K., and Dobrucki J.W. Interaction of a DNA intercalator DRAQ5, and a minor groove binder SYTO17, with chromatin in live cells-influence on chromatin organization and histone-DNA interactions. Cytometry A 73 (2008) 555-562
21. Lang I., Scholz M., and Peters R. Molecular mobility and nucleocytoplasmic flux in hepatoma cells. J. Cell Biol. 102 (1986) 1183-1190
22. Grunwald D., Spottke B., Buschmann V., and Kubitscheck U. Intranuclear binding kinetics and mobility of single native U1 snRNP particles in living cells. Mol. Biol. Cell 17 (2006) 5017-5027