Interaction of a DNA intercalator DRAQ5, and a minor groove binder SYTO17, with chromatin in live cells - Influence on chromatin organization and histone - DNA interactions

Cytometry Part A

Volumn 73, Issue 6, 2008, Pages 555-562

  Wojcik, Krzysztof ^a   Dobrucki, Jurek W ^a  

^a JAGIELLONIAN UNIVERSITY   (Poland)

Author keywords

Chromatin structure; DNA intercalator; DRAQ5; Fluorescence confocal microscopy; Histone exchange; Minor groove binder; Syto17

Indexed keywords

DRAQ5; DYE; HISTONE H1; INTERCALATING AGENT; SYTO17;

ARTICLE; CELL NUCLEUS; CHROMATIN ASSEMBLY AND DISASSEMBLY; CHROMATIN STRUCTURE; CONTROLLED STUDY; DNA HISTONE INTERACTION; HELA CELL; HUMAN; HUMAN CELL; PRIORITY JOURNAL;

ANTHRAQUINONES; CHROMATIN; DNA; FLUORESCENT DYES; HELA CELLS; HISTONES; HUMANS; IMAGE PROCESSING, COMPUTER-ASSISTED; INTERCALATING AGENTS; MICROSCOPY, CONFOCAL; STAINING AND LABELING;

EID: 44949098358     PISSN: 15524922     EISSN: 15524930     Source Type: Journal    
DOI: 10.1002/cyto.a.20573     Document Type: Article

References (17)

1. Njoh KL, Patterson LH, Zloh M, Wiltshire M, Fisher J, Chappell S, Ameer-Beg S, Bai Y, Matthews D, Errington RJ, Smith PJ. Spectral analysis of the DNA targeting bisalkylaminoanthraquinone DRAQ5 in intact living cells. Cytometry Part A 2006;69A:805-814.
2. Kanda T, Sullivan KF, Wahl GM. Histone-GFP fusion protein enables sensitive analysis of chromosome dynamics in living mammalian cells. Curr Biol 1998;8:377-385.
3. Kimura H, Cook PR. Kinetics of core histones in living human cells: Little exchange of H3 and H4 and some rapid exchange of H2B. J Cell Biol 2001;153:1341-1353.
4. Dobrucki JW, Feret D, Noatynska A. Scattering of exciting light by live cells in fluorescence confocal imaging - Phototoxic effects and relevance for FRAP studies. Biophys J 2007;93:1778-1786.
5. Thoma F, Koller T, Klug A. Involvement of histone H1 in the organization of the nucleosome and of the salt-dependent superstructures of chromatin. J Cell Biol 1979;83:403-427.
6. Misteli T, Gunjan A, Hock R, Bustin M, Brown DT. Dynamic binding of histone H1 to chromatin in living cells. Nature 2000;408:877-881.
7. Saenger W. Principles of Nucleic Acid Structure. New York: Springer-Verlag; 1988.
8. Lever MA, Th'ng JP, Sun X, Hendzel MJ. Rapid exchange of histone H1.1 on chromatin in living human cells. Nature 2001;408:873-876.
9. Linder C, Thoma F. Histone H1 expressed in Saccharomyces cerevisiae binds to chromatin and affects survival, growth, transcription, and plasmid stability but does not change nucleosomal spacing. Mol Cell Biol 1994;14:2822-2835.
10. Martin RM, Leonhardt H, Cardoso MC. DNA labeling in living cells. Cytometry Part A 2005;67A:45-52.
11. Varga-Weisz PD, Becker PB. Regulation of higher-order chromatin structures by nucleosome-remodelling factors. Curr Opin Genet Dev 2006;16:151-156.
12. Kulaeva OI, Gaykalova DA, Studitsky VM. Transcription through chromatin by RNA polymerase II: Histone displacement and exchange. Mutat Res 2007;618: 116-129.
13. Hashimoto H, Sonoda E, Takami Y, Kimura H, Nakayama T, Tachibana M, Takeda S, Shinkai Y. Histone H1 variant. H1R is involved in DNA damage response. DNA Repair (Amst) 2007;6:1584-1595.
14. Takata H, Matsunaga S, Morimoto A, Ono-Maniwa R, Uchiyama S, Fukui K. H1.X with different properties from other linker histones is required for mitotic progression. FEBS Lett 2007;581:3783-3788.
15. McClendon AK, Osheroff N. DNA topoisomerase II, genotoxicity, and cancer. Mutat Res 2007;623:83-97.
16. Li HH, Aubrecht J, Fornace AJ Jr. Toxicogenomics: Overview and potential applications for the study of non-covalent DNA interacting chemicals. Mutat Res 2007;623:98-108.
17. Gewirtz D. A Critical evaluation of the mechanisms of action proposed for the antitumor effects of the anthracycline antibiotics adriamycin and daunorubicin. Biochem Pharmacol 1999;57:727-741.