Esterase activity of Bordetella pertussis CyaC-acyltransferase against synthetic substrates: Implications for catalytic mechanism in vivo

FEMS Microbiology Letters

Volumn 304, Issue 2, 2010, Pages 183-190

  Thamwiriyasati, Niramon ^a   Powthongchin, Busaba ^b   Kittiworakarn, Jongrak ^a   Katzenmeier, Gerd ^a   Angsuthanasombat, Chanan ^a  

^a MAHIDOL UNIVERSITY   (Thailand)

^b SILPAKORN UNIVERSITY   (Thailand)

Author keywords

Acyltransferase; Adenylate cyclase hemolysin; Bordetella pertussis; Fatty acyl modification; In vitro activation; Serine esterase

Indexed keywords

4 NITROPHENYL ACETATE; ACETIC ACID DERIVATIVE; ADENYLATE CYCLASE TOXIN; ALANINE; CHYMOTRYPSIN; ESTERASE; HISTAMINE; PALMITIC ACID 4 NITROPHENYL ESTER; PALMITIC ACID DERIVATIVE; SERINE; SERINE PROTEINASE; TYROSINE; UNCLASSIFIED DRUG;

AMINO ACID SEQUENCE; AMINO ACID SUBSTITUTION; ARTICLE; BORDETELLA PERTUSSIS; CATALYSIS; CELL INCLUSION; CONTROLLED STUDY; ENZYME ACTIVE SITE; ENZYME ACTIVITY; ENZYME STRUCTURE; ESCHERICHIA COLI; HYDROLYSIS; IN VITRO STUDY; IN VIVO STUDY; NONHUMAN; NUCLEOTIDE SEQUENCE; PRIORITY JOURNAL; PROTEIN EXPRESSION;

ACETYLTRANSFERASES; ADENYLATE CYCLASE TOXIN; AMINO ACID SEQUENCE; AMINO ACID SUBSTITUTION; BORDETELLA PERTUSSIS; CATALYTIC DOMAIN; ESCHERICHIA COLI; ESTERASES; GENE EXPRESSION; HUMANS; MODELS, CHEMICAL; MODELS, MOLECULAR; MOLECULAR SEQUENCE DATA; MOLECULAR WEIGHT; MUTAGENESIS, SITE-DIRECTED; NITROPHENOLS; PALMITATES; RECOMBINANT PROTEINS; SEQUENCE ALIGNMENT; SUBSTRATE SPECIFICITY;

BORDETELLA PERTUSSIS; ESCHERICHIA COLI;

EID: 77149157491     PISSN: 03781097     EISSN: 15746968     Source Type: Journal    
DOI: 10.1111/j.1574-6968.2010.01896.x     Document Type: Article

References (22)

1. Basar T, Havlicek V, Bezouskova S, Hackett M Sebo P (2001) Acylation of lysine 983 is sufficient for toxin activity of Bordetella pertussis adenylate cyclase. Substitutions of alanine 140 modulate acylation site selectivity of the toxin acyltransferase CyaC. J Biol Chem 276 : 348 354.
2. Bellalou J, Sakamoto H, Ladant D, Geoffroy C Ullmann A (1990) Deletions affecting hemolytic and toxin activities of Bordetella pertussis adenylate cyclase. Infect Immun 58 : 3242 3247.
3. Carbonetti NH, Artamonova GV, Andreasen C Bushar N (2005) Pertussis toxin and adenylate cyclase toxin provide a one-two punch for establishment of Bordetella pertussis infection of the respiratory tract. Infect Immun 73 : 2698 2703.
4. Christen M, Hunenberger PH, Bakowies D et al 2005) The GROMOS software for biomolecular simulation: GROMOS05. J Comput Chem 26 : 1719 1751.
5. Elbaum D Nagel RL (1981) Esterase activity of hemoglobin. Differences between HB A and HB S. J Biol Chem 256 : 2280 2283.
6. Guermonprez P, Khelef N, Blouin E et al 2001) The adenylate cyclase toxin of Bordetella pertussis binds to target cells via the alpha(M)beta(2) integrin (CD11b/CD18). J Exp Med 193 : 1035 1044.
7. Hackett M, Guo L, Shabanowitz J, Hunt DF Hewlett EL (1994) Internal lysine palmitoylation in adenylate cyclase toxin from Bordetella pertussis. Science 266 : 433 435.
8. Hardman MJ, Valenzuela P Bender ML (1971) Acylation of α- and δ-chymotrypsins by p-nitrophenyl acetate. Enzyme-substrate complex formation and pH dependence. J Biol Chem 246 : 5907 5913.
9. Havlicek V, Higgins L, Chen W, Halada P, Sebo P, Sakamoto H Hackett M (2001) Mass spectrometric analysis of recombinant adenylate cyclase toxin from Bordetella pertussis strain 18323/pHSP9. J Mass Spectrom 36 : 384 391.
10. Holmquist M (2000) Alpha/beta-hydrolase fold enzymes: structures, functions and mechanisms. Curr Protein Pept Sc 1 : 209 235.
11. Khelef N, Zychlinsky A Guiso N (1993) Bordetella pertussis induces apoptosis in macrophages: role of adenylate cyclase-hemolysin. Infect Immun 61 : 4064 4071.
12. Lairez D, Pauthe E Pelta J (2003) Refolding of a high molecular weight protein: salt effect on collapse. Biophys J 84 : 3904 3916.
13. Laskowski RA, Rullmannn JA, MacArthur MW, Kaptein R Thornton JM (1996) AQUA and PROCHECK-NMR: programs for checking the quality of protein structures solved by NMR. J Biomol NMR 8 : 477 486.
14. Manavalan P Johnson WC Jr. (1987) Variable selection method improves the prediction of protein secondary structure from circular dichroism spectra. Anal Biochem 167 : 76 85.
15. Masin J, Basler M, Knapp O, El-Azami-El-Idrissi M et al 2005) Acylation of lysine 860 allows tight binding and cytotoxicity of Bordetella adenylate cyclase on CD11b-expressing cells. Biochemistry 44 : 12759 12766.
16. Pluvinage B, Dairou J, Possot OM et al 2007) Cloning and molecular characterization of three arylamine N-acetyltransferase genes from Bacillus anthracis: identification of unusual enzymatic properties and their contribution to sulfamethoxazole resistance. Biochemistry 46 : 7069 7078.
17. Powthongchin B Angsuthanasombat C (2008) High level of soluble expression in Escherichia coli and characterisation of the CyaA pore-forming fragment from a Bordetella pertussis Thai clinical isolate. Arch Microbiol 189 : 169 174.
18. Sebo P Ladant D (1993) Repeat sequences in the Bordetella pertussis adenylate cyclase toxin can be recognized as alternative carboxy-proximal secretion signals by the Escherichia coli alpha-haemolysin translocator. Mol Microbiol 9 : 999 1009.
19. Trent MS, Worsham LM Ernst-Fonberg ML (1999) HlyC, the internal protein acyltransferase that activates hemolysin toxin: roles of various conserved residues in enzymatic activity as probed by site-directed mutagenesis. Biochemistry 38 : 9541 9548.
20. Vriend G (1990) WHAT IF: a molecular modeling and drug design program. J Mol Graphics 8 : 52 56.
21. Westrop GD, Hormozi EK, Da Costa NA, Parton R Coote JG (1996) Bordetella pertussis adenylate cyclase toxin: proCyaA and CyaC proteins synthesised separately in Escherichia coli produce active toxin in vitro. Gene 180 : 91 99.
22. Ziegler K, Yusupov M, Bishop B Born TL (2007) Substrate analysis of homoserine acyltransferase from Bacillus cereus. Biochem Bioph Res Co 361 : 510 515.