Identification of rhesus macaque genital microbiota by 16s pyrosequencing shows similarities to human bacterial vaginosis: Implications for use as an animal model for HIV vaginal infection

AIDS Research and Human Retroviruses

Volumn 26, Issue 2, 2010, Pages 193-200

  Spear, Gregory T ^a   Gilbert, Douglas ^a   Sikaroodi, Masoumeh ^b   Doyle, Lara ^c   Green, Linda ^c   Gillevet, Patrick M ^b   Landay, Alan L ^a   Veazey, Ronald S ^c  

^a RUSH UNIVERSITY MEDICAL CENTER   (United States)

^b GEORGE MASON UNIVERSITY   (United States)

^c TULANE NATIONAL PRIMATE RESEARCH CENTER   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

RNA 16S; SIALIDASE;

ANAEROCOCCUS; ANIMAL EXPERIMENT; ANIMAL MODEL; ARTICLE; ATOPOBACTER; ATOPOBIUM; BACTERIAL STRAIN; CAMPYLOBACTER; CONTROLLED STUDY; CORYNEBACTERIUM; DIALISTER; ENZYME ACTIVITY; FEMALE; HELIOPHILUM; HUMAN IMMUNODEFICIENCY VIRUS INFECTION; HUMAN VERSUS ANIMAL COMPARISON; LACTOBACILLUS; LACTOBACILLUS JOHNSONII; MOBILUNCUS; NONHUMAN; PARVIMONAS; PEPTONIPHILUS; PORPHYROMONAS; PREVOTELLA; PRIORITY JOURNAL; PROTEINIPHILUM; PYROSEQUENCING; RHESUS MONKEY; SNEATHIA; STREPTOCOCCUS; THIOREDUCTOR; VAGINA FLORA; VAGINITIS;

ANIMALS; BACTERIA; DISEASE MODELS, ANIMAL; DNA, BACTERIAL; DNA, RIBOSOMAL; FEMALE; HUMANS; MACACA MULATTA; MONKEY DISEASES; RNA, RIBOSOMAL, 16S; SEQUENCE ANALYSIS, DNA; SIMIAN ACQUIRED IMMUNODEFICIENCY SYNDROME; VAGINA; VAGINOSIS, BACTERIAL;

EID: 77149122114     PISSN: 08892229     EISSN: None     Source Type: Journal    
DOI: 10.1089/aid.2009.0166     Document Type: Article

References (29)

1. Antonio MA, Hawes SE, and Hillier SL: The identification of vaginal Lactobacillus species and the demographic and mi-crobiologic characteristics of women colonized by these species. J Infect Dis 1999;180:1950-1956.
2. Atashili J, Poole C, Ndumbe PM, Adimora AA, and Smith JS: Bacterial vaginosis and HIV acquisition: A meta-analysis of published studies. AIDS 2008;22:1493-1501.
3. Kaul R, Nagelkerke NJ, Kimani J, et al.: Prevalent herpes simplex virus type 2 infection is associated with altered vaginal flora and an increased susceptibility to multiple sexually transmitted infections. J Infect Dis 2007;196:1692-1697.
4. Cherpes TL, Meyn LA, Krohn MA, Lurie JG, and Hillier SL: Association between acquisition of herpes simplex virus type 2 in women and bacterial vaginosis. Clin Infect Dis 2003;37:319-325.
5. Cherpes TL, Hillier SL, Meyn LA, Busch JL, and Krohn MA: A delicate balance: Risk factors for acquisition of bacterial va-ginosis include sexual activity, absence of hydrogen peroxide-producing lactobacilli, black race, and positive herpes simplex virus type 2 serology. Sex Transm Dis 2008;35:78-83.
6. Fredricks DN, Fiedler TL, and Marrazzo JM: Molecular identification of bacteria associated with bacterial vaginosis. N Engl J Med 2005;353:1899-1911.
7. Hyman RW, Fukushima M, Diamond L, Kumm J, Giudice LC, and Davis RW: Microbes on the human vaginal epithelium. Proc Natl Acad Sci USA 2005;102:7952-7957.
8. Han YW, Shen T, Chung P, Buhimschi IA, and Buhimschi CS: Uncultivated bacteria as etiologic agents of intra-amniotic inflammation leading to preterm birth. J Clin Microbiol 2009; 47:38-47.
9. Patton DL, Sweeney YC, Cummings PK, Meyn L, Rabe LK, and Hillier SL: Safety and efficacy evaluations for vaginal and rectal use of BufferGel in the macaque model. Sex Transm Dis 2004;31:290-296.
10. Patton DL, Sweeney YT, Balkus JE, et al.: Preclinical safety assessments of UC781 anti-human immunodeficiency virus topical microbicide formulations. Antimicrob Agents Chemother 2007;51:1608-1615.
11. Patton DL, Sweeney YT, Balkus JE, and Hillier SL: Vaginal and rectal topical microbicide development: Safety and efficacy of 1.0% Savvy (C31G) in the pigtailed macaque. Sex Transm Dis 2006;33:691-695.
12. Ratterree M, Gettie A, Williams V, et al.: Safety and distribution of cellulose acetate 1,2-benzenedicarboxylate (CAP), a candidate anti-HIV microbicide in rhesus macaques. AIDS 2005;19:1595-1599.
13. Schlievert PM, Strandberg KL, Brosnahan AJ, et al.: Glycerol monolaurate does not alter rhesus macaque (Macaca mu-latta) vaginal lactobacilli and is safe for chronic use. Antimicrob Agents Chemother 2008;52:4448-4454.
14. Doyle L, Young CL, Jang SS, and Hillier SL: Normal vaginal aerobic and anaerobic bacterial flora of the rhesus macaque (Macaca mulatta). J Med Primatol 1991;20:409-413.
15. Lane DJ: 16S/23S rRNA sequencing. In: Goodfellow ESaM, Ed. Nucleic Acid Techniques in Bacterial Systematics (pp. 115-175). West Sussex, England: John Wiley & Sons Ltd, 1991.
16. Cole JR, Chai B, Farris RJ, et al.: The Ribosomal Database Project (RDP-II): Sequences and tools for high-throughput rRNA analysis. Nucleic Acids Res 2005;33:294-296.
17. Barman M, Unold D, Shifley K, et al.: Enteric salmonellosis disrupts the microbial ecology of the murine gastrointestinal tract. Infect Immun 2008;76:907-915.
18. Briselden AM, Moncla BJ, Stevens CE, and Hillier SL: Sialidases (neuraminidases) in bacterial vaginosis and bacterial vaginosis-associated microflora. J Clin Microbiol 1992;30: 663-666.
19. Spear GT, Sikaroodi M, Zariffard MR, Landay AL, French AL, and Gillevet PM: Comparison of the diversity of the vaginal microbiota in HIV-infected and HIV-uninfected women with or without bacterial vaginosis. J Infect Dis 2008;198:1131-1140.
20. Spiegel CA, Davick P, Totten PA, et al.: Gardnerella vaginalis and anaerobic bacteria in the etiology of bacterial (nonspecific) vaginosis. Scand J Infect Dis Suppl 1983;40:41-46.
21. Hill GB: The microbiology of bacterial vaginosis. Am J Obstet Gynecol 1993;169:450-454.
22. Fredricks DN, Fiedler TL, Thomas KK, Oakley BB, and Marrazzo JM: Targeted PCR for detection of vaginal bacteria associated with bacterial vaginosis. J Clin Microbiol 2007;45:3270-3276.
23. Boskey ER, Telsch KM, Whaley KJ, Moench TR, and Cone RA: Acid production by vaginal flora in vitro is consistent with the rate and extent of vaginal acidification. Infect Immun 1999;67:5170-5175.
24. Hillier SL: The vaginal microbial ecosystem and resistance to HIV. AIDS Res Hum Retroviruses 1998;14(Suppl 1):S17-21.
25. Spear GT, St John E, and Zariffard MR: Bacterial vaginosis and human immunodeficiency virus infection. AIDS Res Ther 2007;4:25.
26. Allsworth JE, Lewis VA, and Peipert JF: Viral sexually transmitted infections and bacterial vaginosis: 2001-2004. National Health and Nutrition Examination Survey data. Sex Transm Dis 2008;35:791-796.
27. Marrazzo JM, Thomas KK, Fiedler TL, Ringwood K, and Fredricks DN: Relationship of specific vaginal bacteria and bacterial vaginosis treatment failure in women who have sex with women. Ann Intern Med 2008;149:20-28.
28. Leitich H, Bodner-Adler B, Brunbauer M, Kaider A, Egarter C, and Husslein P: Bacterial vaginosis as a risk factor for preterm delivery: A meta-analysis. Am J Obstet Gynecol 2003;189:139-147.
29. Cherpes TL, Melan MA, Kant JA, Cosentino LA, Meyn LA, and Hillier SL: Genital tract shedding of herpes simplex virus type 2 in women: Effects of hormonal contraception, bacterial vaginosis, and vaginal group B Streptococcus colonization. Clin Infect Dis 2005;40:1422-1428.