Directed evolution of copper nitrite reductase to a chromogenic reductant

Protein Engineering, Design and Selection

Volumn 23, Issue 3, 2010, Pages 137-145

  MacPherson, Iain S ^a,b   Rosell, Federico I ^a   Scofield, Melanie ^a   Mauk, A Grant ^a   Murphy, Michael E P ^a  

^a UNIVERSITY OF BRITISH COLUMBIA   (Canada)

^b BRANDEIS UNIVERSITY   (United States)

Author keywords

Colony screen; Crystallography; Directed evolution; Electrochemistry; Nitrite reductase

Indexed keywords

ALCALIGENES FAECALIS; CHROMOGENIC SUBSTRATE; COPPER NITRITE REDUCTASE; DIOXYGENS; DIRECTED EVOLUTION; DIRECTED EVOLUTION METHODS; EFFICIENT PRODUCTION; ELECTRON ACCEPTOR; FUNCTIONAL CHARACTERIZATION; HIGH-THROUGHPUT; LIBRARY GENERATION; NITRITE REDUCTASE; NYLON MEMBRANES; O-DIANISIDINE; PCR CLONING; REDUCTANTS; SCREENING METHODS; SYNTHESIS REACTION;

CLONING; CRYSTALLOGRAPHY; ELECTROCHEMISTRY; GENE EXPRESSION; MINERALOGY; OXIDATION; PLANTS (BOTANY); SYNTHESIS (CHEMICAL);

SUBSTRATES;

4 DIMETHYLAMINOAZOBENZENE; CHROMOGENIC SUBSTRATE; DIANISIDINE; NITRITE REDUCTASE; OXYGEN;

ALCALIGENES FAECALIS; ARTICLE; CLONING; COLOR; CONTROLLED STUDY; ENZYME ANALYSIS; ENZYME METABOLISM; HIGH THROUGHPUT SCREENING; LIBRARY; LYSIS; MOLECULAR EVOLUTION; NONHUMAN; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; PROTEIN EXPRESSION; PROTEIN FUNCTION;

ALCALIGENES FAECALIS; AZURIN; CHROMOGENIC COMPOUNDS; COPPER; CRYSTALLOGRAPHY, X-RAY; DIANISIDINE; DIRECTED MOLECULAR EVOLUTION; ELECTROCHEMISTRY; ELECTRONS; ENZYME ASSAYS; HIGH-THROUGHPUT SCREENING ASSAYS; MODELS, MOLECULAR; MUTAGENESIS, SITE-DIRECTED; MUTATION; NITRITE REDUCTASES; OXIDATION-REDUCTION; OXYGEN; PROTEIN CONFORMATION; REDUCING AGENTS; REPRODUCIBILITY OF RESULTS; SPECTRUM ANALYSIS;

ALCALIGENES FAECALIS;

EID: 77049109707     PISSN: 17410126     EISSN: 17410134     Source Type: Journal    
DOI: 10.1093/protein/gzp084     Document Type: Article

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