A comparison of six methods for genomic DNA extraction suitable for PCR-based genotyping applications using ovine milk samples

Molecular and Cellular Probes

Volumn 24, Issue 2, 2010, Pages 93-98

  Psifidi, Androniki ^a   Dovas, Chrysostomos I ^a   Banos, Georgios ^a  

^a ARISTOTLE UNIVERSITY OF THESSALONIKI   (Greece)

Author keywords

Bulk milk; Dairy sheep; DNA extraction; Real time PCR

Indexed keywords

CHLOROFORM; GENOMIC DNA; PHENOL;

ANALYTICAL EQUIPMENT; ARTICLE; CONTROLLED STUDY; DNA EXTRACTION; DNA ISOLATION; GEL ELECTROPHORESIS; INTERMETHOD COMPARISON; MILK; NONHUMAN; PRIORITY JOURNAL; REAL TIME POLYMERASE CHAIN REACTION; SHEEP; SPECTROPHOTOMETER;

ANIMALS; COSTS AND COST ANALYSIS; DNA; ELECTROPHORESIS, AGAR GEL; GENOME; GENOTYPE; MILK; REFERENCE STANDARDS; REVERSE TRANSCRIPTASE POLYMERASE CHAIN REACTION; SHEEP; SPECTROPHOTOMETRY; TIME FACTORS;

ANIMALIA; OVIS; OVIS ARIES;

EID: 76849103311     PISSN: 08908508     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.mcp.2009.11.001     Document Type: Article

References (26)

1. D'Angelo F., Santillo A., Sevi A., and Albenzio M. Technical note: A simple salting-out method for DNA extraction from milk somatic cells: investigation into the goat CSN1S1 Gene. J Dairy Sci 90 (2007) 3550-3552
2. Psifidi A, Dovas CI, Basdagianni Z, Bouzalas I, Banos G. Assessing scrapie risk in daity sheep flocks. In: Proceedings of the 2009 annual meeting british society of animal science, Scarborough, UK, 31 March-2 April, 2008, p. 25.
3. Bagnato A., Schiavini F., Rossoni A., Maltecca C., Dolezal M., Medugorac I., et al. Quantitative trait loci affecting milk yield and protein percentage in a three-country Brown Swiss population. J Dairy Sci 91 (2008) 767-783
4. Buitkamp J., and Gotz K. Use of milk samples from a milk evaluation programme for the genotyping of cows. Arch Tierz Dummerstorf 47 (2004) 15-26
5. Lipkin E., Shalom A., Khatib H., Soller M., and Friendmann A. Milk as a source of deoxyribonucleic acid and as a substrate for the polymerase chain reaction. J Dairy Sci 76 (1993) 2025-2032
6. Lindquist S., Hanson L., Hernell O., Lonneral B., Normack J., Stromqvist M., et al. Isolation of mRNA and genomic DNA from epithelial cells in human milk and amplified by PCR. Biotechniques 17 (1994) 692
7. Amills M., Francino O., Jansa M., and Sanchez A. Isolation of genomic DNA from milk samples by using Chelex resin. J Dairy Sci 64 (1997) 231-238
8. Murphy M.A., Shariflu M.R., and Moran C. High quality genomic DNA extraction from large milk samples. J Dairy Res 69 (2002) 645-649
9. Lopez Calleja I., Gonzalez I., Fajardo V., Rotriquez M.A., Hermandez P.E., Garcia T., et al. Rapid detection of cows' milk in sheeps' and goats' milk by a species-specific polymerase chain reaction technique. J Dairy Sci 87 (2004) 2839-2845
10. Ensminger M.E. Dairy cattle science. 3rd ed. (1993), Interstate Publishers Inc
11. Wilson I.G. Inhibition and facilitation of nucleic acid amplification. Appl Environ Microbiol 63 (1997) 3741-3751
12. Borodina T., Lehrach H., and Soldator A. DNA purification on homemade silica spin columns. Anal Biochem 321 1 (2003) 135-137
13. Black J.A., and Foarde K.K. Comparison of four different methods for extraction of Stachybotrys chartarum spore DNA and verification by real-time PCR. J Microbiol Methods 70 (2007) 75-81
14. Smith K., Diggle M.A., and Clarke S.C. Comparison of commercial DNA extraction kits for extraction of bacterial genomic DNA from whole-blood samples. J Clinical Microbiol (June 2003) 2440-2443
15. Extramiana B., González L., Cortabarría N., García M., and Juste R. Evaluation of a PCR technique for the detection of maedi visna proviral DNA in blood, milk and tissue samples of naturally infected sheep. Small Ruminant Res 44 (2002) 109-117
16. Fahle G.A., and Fischer S.H. Comparison of six commercial extraction kits for recovery of Cytomegalovirus DNA from spiked human specimens. J Clinical Microbiol (October 2000) 3860-3863
17. Riemann K., Adamzik M., Frauenrath S., Egensperger R., Schmid K.W., Brockmeyer N.H., et al. Comparison of manual and automated nucleic acid extraction from whole-blood samples. J Clin Lab Anal 21 (2007) 244-248
18. Kong W.J., Ying W., Qiong W., Yue-chen H., and Yu-Juan H. Comparison of three methods for isolation of nucleic acids from membranate inner ear tissue of rats. China Med J 119 12 (2006) 889-900
19. Keegan H., Boland C., Malkin A., Griffin M., Ryan F., and Lambkin H. Comparison of DNA extraction from cervical cells collected in PreserCyt solution for the amplification of Chlamydia trachomatis. Cytopathology 16 (2005) 82-87
20. Leslie C., Bu D., Lewis C., and Euhus D. A comparison of five methods for extracting DNA from paucicellular clinical samples. Mol Cell Probes 20 (2006) 191-196
21. Griffiths L.J., Anyim M., Doffman S.R., Millar M.R., and Agrawal S.G. Comparison of DNA extraction methods for Aspergillus fumigatus using real-time PCR. J Med Microbiol 55 (2006) 1187-1191
22. Clausen F.B., Krog G.R., Rieneck K., and Dziegiel M.H. Improvement in fetal DNA extraction of maternal plasma. Evaluation of the Nuclisens Magnetic Extraction system and the QIAamp DSP Virus Kit in comparison with the QIAamp DNA Blood Kit. Prenat Diagn 27 (2007) 6-10
23. Clements D.N., Wood S., Carter S., and Ollier W.E. Assessment of the quality and quantity of genomic DNA recovered from canine blood samples by three different extraction methods. Res Vet Sci 85 (2008) 74-79
24. 171 is associated with high susceptibility to scrapie in purebred and crossbred German Merinoland sheep. Arch Virol 149 (2004) 1571-1580
25. Nolan T., Hands R.E., Ogunkolade W., and Bustin S.A. SPUD: a quantitative PCR assay for the detection of inhibitors in nucleic acid preparations. Anal Biochem 351 (2006) 308-310
26. Leitner G., Chaffer M., Caraso C., Ezra E., Kababea D., Winkler M., et al. Udder infection and milk somatic cell count, NAGase activity and milk composition-fat, protein and lactose in Israeli Assaf and Awassi sheep. Small Ruminant Res 39 (2003) 107-112