Genetic interventions in mammalian cells; Applications and uses in high-throughput screening and drug discovery

Cell Biology and Toxicology

Volumn 26, Issue 1, 2010, Pages 43-55

  Hampton, Sally L ^a   Kinnaird, Anne I ^a  

^a Vantia Ltd   (United Kingdom)

Author keywords

Cellular assays; Drug discovery; GPCR; Target manipulation

Indexed keywords

CELL ASSAY; CELL LINE; DNA MODIFICATION; DRUG DELIVERY SYSTEM; DRUG RESEARCH; DRUG TARGETING; GENETICS; HIGH THROUGHPUT SCREENING; HUMAN; IN VITRO STUDY; MAMMAL CELL; NONHUMAN; PRIORITY JOURNAL; REVIEW;

ANIMALS; CELL LINE; DRUG DISCOVERY; DRUG EVALUATION, PRECLINICAL; GENE EXPRESSION; GENE EXPRESSION REGULATION; GENE TRANSFER TECHNIQUES; GENES; GENETIC ENGINEERING; HIGH-THROUGHPUT SCREENING ASSAYS; HUMANS; MAMMALS;

MAMMALIA;

EID: 76749161255     PISSN: 07422091     EISSN: None     Source Type: Journal    
DOI: 10.1007/s10565-009-9140-z     Document Type: Review

References (18)

1. CP Adams VV Brantner 2006 Estimating the cost of new drug development: is it really 802 million dollars? Health Aff (Millwood) 25 2 420 428 10.1377/hlthaff.25.2.420
2. IJ Beresford, et al. 1995 GR159897, a potent non-peptide antagonist at tachykinin NK2 receptors Eur J Pharmacol 272 2-3 241 248 10.1016/0014-2999(94) 00655-Q 1:CAS:528:DyaK2MXjt1Smsrw%3D 7713168
3. UAK Betz 2005 How many genomics targets can a portfolio afford? Drug Discov Today 10 15 1057 1063 10.1016/S1359-6446(05)03498-7 16055022 (Pubitemid 41074144)
4. C Bradburne K Robertson D Thach 2009 Assessment of methods and analysis of outcomes for comprehensive optimization of nucleofection Genet Vaccines Ther 7 6 10.1186/1479-0556-7-6 19432988
5. D Cawkill SS Eaglestone 2007 Evolution of cell-based reagent provision Drug Discov Today 12 19-20 820 825 10.1016/j.drudis.2007.08.014 1:CAS:528:DC%2BD2sXhtFKitrfL 17933682 (Pubitemid 47539971)
6. NL Craig 1988 The mechanism of conservative site-specific recombination Ann Rev Genet 22 77 105 10.1146/annurev.ge.22.120188.000453 1:CAS:528: DyaL1MXhs1Krt7g%3D 3071260
7. CY Han, et al. 2008 Nucleofection is a highly effective gene transfer technique for human melanoma cell lines Exp Dermatol 17 5 405 411 10.1111/j.1600-0625.2007.00687.x 1:CAS:528:DC%2BD1cXmslKlt7s%3D 18312380
8. SJ Hill, et al. 2001 Reporter-gene systems for the study of G-protein-coupled-receptors Curr Opin Pharmacol 1 526 532 10.1016/S1471-4892(01) 00091-1 1:CAS:528:DC%2BD3MXnsFOisr8%3D 11764780 (Pubitemid 33593844)
9. Human Genome Sequencing Consortium 2004 Finishing the euchromatic sequence of the human genome Nature 431 7011 931 945 10.1038/nature03001 (Pubitemid 39434409)
10. E Kostenis M Waelbroeck G Milligan 2005 Techniques: promiscuous Galpha proteins in basic research and drug discovery Trends Pharmacol Sci 26 11 595 602 10.1016/j.tips.2005.09.007 1:CAS:528:DC%2BD2MXhtFKhsrrM 16183138 (Pubitemid 41505228)
11. P Kunapuli, et al. 2005 Application of division arrest technology to cell-based HTS: comparison with frozen and fresh cells Assay Drug Dev Technol 3 1 17 26 10.1089/adt.2005.3.17 1:CAS:528:DC%2BD2MXislagu7Y%3D 15798392 (Pubitemid 40533249)
12. CA Maggi R Patacchini P Rovero A Giachetti 1993 Tachykinin receptors and tachykinin receptor antagonists J Auton Pharmacol 13 1 23 93 10.1111/j.1474-8673.1993.tb00396.x 1:CAS:528:DyaK3sXhtl2qu74%3D 8382703
13. Masia N. The cost of developing a new drug. In: US Department of State, editor. Focus on: intellectual property rights. Washington, DC: US Department of State; 2006. p. 82-3.
14. DJ McLoughlin F Bertelli C Williams 2007 The A, B, Cs of G-protein-coupled receptor pharmacology in assay development for HTS Expert Opin Drug Discov 2 5 603 619 10.1517/17460441.2.5.603 1:CAS:528:DC%2BD2sXotFyntbc%3D (Pubitemid 47071655)
15. JP Overington B Al-Lazikani AL Hopkins 2006 How many drug targets are there? Nat Rev Drug Discov 5 993 996 10.1038/nrd2199 1:CAS:528: DC%2BD28Xht1Kju7jM 17139284 (Pubitemid 44835126)
16. B Sauer 1994 Site-specific recombination: developments and applications Curr Opin Biotechnol 5 521 527 10.1016/0958-1669(94)90068-X 1:CAS:528: DyaK2MXhvV2gu70%3D 7765467 (Pubitemid 24321421)
17. C Venter, et al. 2001 The sequence of the human genome Science 291 1304 1351 10.1126/science.1058040 1:CAS:528:DC%2BD3MXhtlSgsbo%3D 11181995 (Pubitemid 32173090)
18. MJ Wigglesworth, et al. 2008 Use of cryopreserved cells for enabling greater flexibility in compound profiling J Biomol Screen 13 5 354 362 10.1177/1087057108317768 1:CAS:528:DC%2BD1cXotlCms7g%3D 18467669 (Pubitemid 351793986)