Effects of reduced oxygen and glucose levels on ocular cells in vitro: Implications for tissue models

Cells Tissues Organs

Volumn 191, Issue 2, 2010, Pages 141-151

  Sander, Edward A ^a,b,c   Nauman, Eric A ^b,c,d  

^a Tulane University   (United States)

^b PURDUE UNIVERSITY   (United States)

^c PURDUE UNIVERSITY   (United States)

^d PURDUE UNIVERSITY   (United States)

Author keywords

Aerobic cell metabolism; Anaerobic metabolism; Astrocytes; Fibroblasts; Glaucoma; Lamina cribrosa; Sclera

Indexed keywords

GLUCOSE; GLUTAMIC ACID; LACTIC ACID; OXYGEN; PYRUVIC ACID;

ANIMAL CELL; ARTICLE; ASTROCYTE; CELL COUNT; CELL TYPE; CONTROLLED STUDY; FIBROBLAST; GLAUCOMA; IN VITRO STUDY; METABOLISM; NONHUMAN; PRIORITY JOURNAL; RAT; SCLERA; SWINE; TISSUE ENGINEERING;

ANIMALS; ASTROCYTES; CELL PROLIFERATION; CELLS, CULTURED; GLAUCOMA, OPEN-ANGLE; GLUCOSE; GLUTAMIC ACID; LACTIC ACID; MODELS, BIOLOGICAL; OPTIC DISK; OXYGEN; PYRUVIC ACID; RATS; SCLERA; SWINE; TISSUE ENGINEERING;

ANIMALIA; RATTUS; SUS;

EID: 76749111471     PISSN: 14226405     EISSN: None     Source Type: Journal    
DOI: 10.1159/000231471     Document Type: Article

References (33)

1. Bellezza, A.J., R.T. Hart, C.F. Burgoyne (2000) The optic nerve head as a biomechanical structure: initial finite element modeling. Invest Ophthalmol Vis Sci 41: 2991-3000.
2. Bergmeyer, H.U. (1983) Methods of Enzymatic Analysis. Weinheim, Verlag Chemie.
3. Blaheta, R.A., M. Franz, M.K. Auth, H.J. Wenisch, B.H. Markus (1991) A rapid nonradioactive fluorescence assay for the measurement of both cell number and proliferation. J Immunol Methods 142: 199-206.
4. Brand, K. (1997) Aerobic glycolysis by proliferating cells: protection against oxidative stress at the expense of energy yield. J Bioenerg Biomembr 29: 355-364.
5. Burgoyne, C.F., J.C. Downs, A.J. Bellezza, J.K. Suh, R.T. Hart (2005) The optic nerve head as a biomechanical structure: a new paradigm for understanding the role of IOP-related stress and strain in the pathophysiology of glaucomatous optic nerve head damage. Prog Retin Eye Res 24: 39-73.
6. Cardinal, K.O., G.T. Bonnema, H. Hofer, J.K. Barton, S.K. Williams (2006) Tissue-engineered vascular grafts as in vitro blood vessel mimics for the evaluation of endothelialization of intravascular devices. Tissue Eng 12: 3431-3438.
7. Clark, A.F., S. Browder, H.T. Steely, K. Wilson, D. Cantu-Crouch, M.D. McCartney (1995) Cell biology of the human lamina cribrosa; in Drance, S.M. (ed): Optic Nerve in Glaucoma. Amsterdam, Kugler, pp 79-105.
8. Csete, M. (2005) Oxygen in the cultivation of stem cells. Ann NY Acad Sci 1049: 1-8.
9. Europa, A.F., A. Gambhir, P.C. Fu, W.S. Hu (2000) Multiple steady states with distinct cellular metabolism in continuous culture of mammalian cells. Biotechnol Bioeng 67: 25-34.
10. Fermor, B., J. Urban, D. Murray, A. Pocock, E. Lim, M. Francis, J. Gage (1998) Proliferation and collagen synthesis of human anterior cruciate ligament cells in vitro: effects of ascorbate-2-phosphate, dexamethasone and oxygen tension. Cell Biol Int 22: 635-640.
11. Foster, D.W., A.H. Rubenstein (1994) Hypoglycemia; in Isselbacher, K.J., E. Braunwald, J.D. Wilson, J.B. Martin, A.S. Fauci, D.L. Kasper (eds): Harrison's Principles of Internal Medicine. New York, McGraw-Hill, pp 2000- 2006.
12. Freshney, R.I. (2000) Culture of Animal Cells: A Manual of Basic Technique. New York, Wiley-Liss.
13. Griffith, L.G., G. Naughton (2002) Tissue engineering - current challenges and expanding opportunities. Science 295: 1009-1014.
14. Gstraunthaler, G., T. Seppi, W. Pfaller (1999) Impact of culture conditions, culture media volumes, and glucose content on metabolic properties of renal epithelial cell cultures. Are renal cells in tissue culture hypoxic? Cell Physiol Biochem 9: 150-172.
15. Hansen, U., M. Schunke, C. Domm, N. Ioannidis, J. Hassenpflug, T. Gehrke, B. Kurz (2001) Combination of reduced oxygen tension and intermittent hydrostatic pressure: a useful tool in articular cartilage tissue engineering. J Biomech 34: 941-949.
16. Hernandez, M.R. (2000) The optic nerve head in glaucoma: role of astrocytes in tissue remodeling. Prog Retin Eye Res 19: 297-321.
17. Hernandez, M.R., F. Igoe, A.H. Neufeld (1988) Cell culture of the human lamina cribrosa. Invest Ophthalmol Vis Sci 29: 78-89.
18. Hernandez, M.R., X.X. Luo, W. Andrzejewska, A.H. Neufeld (1989) Age-related changes in the extracellular matrix of the human optic nerve head. Am J Ophthalmol 107: 476-484.
19. Hu, W.S., M.G. Oberg (1990) Monitoring and control of animal cell bioreactors: Biochemical engineering considerations. Bioprocess Technol 10: 451-481.
20. Ignatius, A., H. Blessing, A. Liedert, C. Schmidt, C. Neidlinger-Wilke, C. Kaspar, B. Friemert, L. Claes (2005) Tissue engineering of bone: effects of mechanical strain on osteoblastic cells in type I collagen matrices. Biomaterials 26: 311-318.
21. Lambert, W., R. Agarwal, W. Howe, A.F. Clark, R.J. Wordinger (2001) Neurotrophin and neurotrophin receptor expression by cells of the human lamina cribrosa. Invest Ophthalmol Vis Sci 42: 2315-2323.
22. Neermann, J., R. Wagner (1996) Comparative analysis of glucose and glutamine metabolism in transformed mammalian cell lines, insect and primary liver cells. J Cell Physiol 166: 152-169.
23. Obradovic, B., R.L. Carrier, G. Vunjak-Novakovic, L.E. Freed (1999) Gas exchange is essential for bioreactor cultivation of tissue engineered cartilage. Biotechnol Bioeng 63: 197-205.
24. Planchet, E., K. Jagadis Gupta, M. Sonoda, W.M. Kaiser (2005) Nitric oxide emission from tobacco leaves and cell suspensions: Rate limiting factors and evidence for the involvement of mitochondrial electron transport. Plant J 41: 732-743.
25. Richards, W.L., M.K. Song, H. Krutzsch, R.P. Evarts, E. Marsden, S.S. Thorgeirsson (1985) Measurement of cell proliferation in microculture using Hoechst 33342 for the rapid semiautomated microfluorimetric determination of chromatin DNA. Exp Cell Res 159: 235-246.
26. Shimko, D.A., K.K. White, E.A. Nauman, K.C. Dee (2003) A device for long term, in vitro loading of three-dimensional natural and engineered tissues. Ann Biomed Eng 31: 1347-1356.
27. Sivaraman, A., J.K. Leach, S. Townsend, T. Iida, B.J. Hogan, D.B. Stolz, R. Fry, L.D. Samson, S.R. Tannenbaum, L.G. Griffith (2005) A microscale in vitro physiological model of the liver: Predictive screens for drug metabolism and enzyme induction. Curr Drug Metab 6: 569-591.
28. Smith, M.K., D.J. Mooney (2007) Hypoxia leads to necrotic hepatocyte death. J Biomed Mater Res A 80: 520-529.
29. Stefansson, E., M.B. Landers 3rd, M. Wolbarsht (2005a) Retinal vessel caliber and diabetic retinopathy. Arch Ophthalmol 123: 709.
30. Stefansson, E., D.B. Pedersen, P.K. Jensen, M. la Cour, J.F. Kiilgaard, K. Bang, T. Eysteinsson (2005b) Optic nerve oxygenation. Prog Retin Eye Res 24: 307-332.
31. Suuronen, E.J., C.R. McLaughlin, P.K. Stys, M. Nakamura, R. Munger, M. Griffith (2004) Functional innervation in tissue engineered models for in vitro study and testing purposes. Toxicol Sci 82: 525-533.
32. Tjabringa, G., M. Bergers, D. van Rens, R. de Boer, E. Lamme, J. Schalkwijk (2008) Development and validation of human psoriatic skin equivalents. Am J Pathol 173: 815-823.
33. Zielke, H.R., P.T. Ozand, J.T. Tildon, D.A. Sevdalian, M. Cornblath (1978) Reciprocal regulation of glucose and glutamine utilization by cultured human diploid fibroblasts. J Cell Physiol 95: 41-48.