Hypoxia and kinase activity regulate lung epithelial cell glutathione

Experimental Lung Research

Volumn 36, Issue 1, 2010, Pages 45-56

  Jackson, Robert M ^a,b   Gupta, Chhavi ^b  

^a Miami VAHS   (United States)

^b UNIVERSITY OF MIAMI   (United States)

Author keywords

Antioxidants; Epithelial; Hypoxia; Kinases; Lung; Oxidants

Indexed keywords

2 (2 AMINO 3 METHOXYPHENYL)CHROMONE; 4 (4 FLUOROPHENYL) 2 (4 METHYLSULFINYLPHENYL) 5 (4 PYRIDYL)IMIDAZOLE; ACETYLCYSTEINE; ADENOVIRUS VECTOR; CATALASE; GLUTAMATE CYSTEINE LIGASE; GLUTATHIONE; GLUTATHIONE SYNTHASE; HEAT SHOCK PROTEIN 27; MITOGEN ACTIVATED PROTEIN KINASE; MITOGEN ACTIVATED PROTEIN KINASE P38; PHOSPHOTRANSFERASE;

ANIMAL EXPERIMENT; ANIMAL MODEL; ANIMAL TISSUE; ARTICLE; CELL HYPOXIA; CONTROLLED STUDY; DOWN REGULATION; ENZYME ACTIVITY; ENZYME ASSAY; ENZYME REGULATION; ENZYME SYNTHESIS; EPITHELIUM CELL; GENE OVEREXPRESSION; GENETIC TRANSFECTION; HUMAN; HUMAN CELL; IN VIVO CULTURE; LIVER; LUNG; LUNG ALVEOLUS EPITHELIUM; MALE; MOUSE; NONHUMAN; PRIORITY JOURNAL; PROTEIN CONTENT; PROTEIN EXPRESSION; PROTEIN PHOSPHORYLATION; WESTERN BLOTTING;

ANIMALS; ANOXIA; CATALASE; DOWN-REGULATION; EPITHELIAL CELLS; GLUTAMATE-CYSTEINE LIGASE; GLUTATHIONE; GLUTATHIONE SYNTHASE; HUMANS; LUNG; MAP KINASE SIGNALING SYSTEM; MICE; MICE, KNOCKOUT; P38 MITOGEN-ACTIVATED PROTEIN KINASES;

EID: 76749101154     PISSN: 01902148     EISSN: 15210499     Source Type: Journal    
DOI: 10.3109/01902140903061795     Document Type: Article

References (34)

1. Cantin A, North S, Hubbard R, Crystal R: Normal alveolar epithelial lining fluid contains high levels of glutathione. J Appl Physiol. 1987;63:152-157.
2. Guidot D, Modelska K, Lois M, Jain L, Moss I, Pittet J, et al.: Ethanol ingestion via glutathione depletion impairs alveolar epithelial barrier function in rats. Am J Physiol Lung Cell Mol Physiol. 2000;279:L127-L135.
3. Jackson R, Garcia-Rojas R: Kinase activity, Hsp27 phosphorylation and lung epithelial cell glutathione. Exp Lung Res. 2008;34:245-262.
4. Salinthone S, BaM, Hanson L,Martin J, Halayko A, Gerthoffer W: Over expression of human Hsp27 inhibits seruminduced proliferation in airway smooth muscle myocytes and confers resistance to hydrogen peroxide cytotoxicity. Am J Physiol Lung Cell Mol Physiol. 2007;293:L1194-L1207.
5. Mehlen P, Hickey E, Weber L, Arrigo A: Large unphosphorylated aggregates as the active form of Hsp27, which controls intracellular reactive oxygen species and glutathione levels and generates a protection against TNF-α in NIH-3T3-ras cells. Biochem Biophys Res Commun. 1997;241:187-192.
6. Martin-Ventura J, Nicolas V, Houard X, Blanco-Colio L, Leclercq A, Egido J, et al.: Biological significance of decreased Hsp27 in human atherosclerosis. Arterioscler Thromb Vasc Biol. 2006;26:1337-1343.
7. Kotlyarov A, Neininger A, Schubert C, Eckert R, Birchmeier C, Volk H, GaestelM:MAPKAP kinase 2 is essential for LPSinduced TNF-α biosynthesis. Nat Cell Biol. 1999;1:94-97.
8. Balis J, Bumganer S, Paciga J, Paterson J, Shelley S: Synthesis of lung surfactant-associated glycoproteins by A549 cells: description of an in vitro model for human type II cell dysfunction. Exp Lung Res. 1984;6:197-213.
9. Lazrak A, Samanta A, Matalon S: Biophysical and molecular characterization of amiloride-sensitive sodium channels in A549 cells. Am J Lung Cell Mol Physiol. 2000;278:L848-L857.
10. Watanabe N, Dickinson D, Kryzywanski D, Iles K, Zhang H, Venglarik C, Forman H: A549 subclones demonstrate heterogeneity toxicological sensitivity and antioxidant profile. Am J Lung Cell Mol Physiol. 2002;283:L726-736.
11. Powell C, Wright M, Jackson R: Mitochondrial complex I and aconitase during hypoxia-reoxygenation: Modulation of enzyme activities by mitochondrial MnSOD augmentation. Am J Physiol (Lung Cell Mol Physiol). 2003;285:L189-L198.
12. Farriss M, Read D: High performance liquid chromatography of thiols and disulfides: dinitrophenol derivatives. Methods Enzymol. 1987;143:101-109.
13. Bai J, Cederbaum A: Adenovirus-mediated overexpression of catalase in the cytosolic or mitochondrial compartment protects against cytochrome P450 2E1-dependent toxicity in HepG2 cells. J Biol Chem. 2001;276:4315-4321.
14. Liu T, Warburton R, Guevara O, Hill N, Fanburg B, Gaestel M, Kayyali U: Lack of MK2 myofibroblast formation and exacerbates pulmonary fibrosis. Am J Respir Cell Mol Biol. 2007;37:507-517.
15. Vergauwen B, de Vos D, van Beeumen J: Characterization of the bifunctional γ -glutamate-cysteine ligase/glutathione synthetase (GshF) of Pasteurella multocida. J Biol Chem. 2006;2817:4380-4394.
16. Burnette W: "Western blotting": electrophoretic transfer of proteins from sodium dodecyl sulfate-polyacrylamide gels to unmodified nitrocellulose and radiographic detection with antibody and radio iodinated protein A. Anal Biochem. 1981;112:195-203.
17. Tietze F: Enzymic method for quantitative determination of nanogram amounts of total and oxidized glutathione: applications to mammalian blood and other tissues. Anal Biochem. 1969;27:502-520.
18. Ludbrook J: Multiple comparison procedures updated. Clin Exp Pharmacol Physiol. 1998;25:1032-1037.
19. Dickinson D, Forman H: Cellular glutathione and thiols metabolism. Biochem Pharmacol. 2002;64:1019-1026.
20. Mansfield K, Simon M, Keith B: Hypoxic reduction in cellular glutathione levels requires mitochondrial reactive oxygen species. J Appl Physiol. 2004;97:1358-1366.
21. Bell E, Klimova T, Eisenbart J, Moraes C, Murphy M, Budinger G, Chandel N: The Q0 site of the mitochondrial complex III is required for the transduction on hypoxic signaling via reactive oxygen species production. J Cell Biol. 2007;177:1029-1036.
22. Seelig G, Simondsen R, Meister A: Reversible dissociation of γ -glutamylcysteine synthetase into two subunits. J Biol Chem. 1984;259:9345-9347.
23. Tu Z, Anders M: Expression and characterization of human glutamate-cysteine ligase. Arch Biochem Biophys. 1998;354:247-254.
24. Yang H, Zeng Y, Lee T, Yang Y, Ou X, Chen L, Haque M, Rippe R, Lu S: Role of AP-1 in the coordinate regulation of rat glutamate cysteine ligase and glutathione synthetase by tertbutyl hydroquinone. J Biol Chem. 2002;277:35232-35239.
25. Lu S: Regulation of glutathione synthesis. Mol Mech Med. 2009;30:42-59.
26. Janssen-Heininger Y, Persinger R, Korn S, Pantano C, McElhinney B, ReynaertN, Lange R, Ckless K, Shrivastava P, Poynter M: Reactive nitrogen species and cell signaling. Implications for cell death or survival of lung epithelium. Am J Resp Crit Care Med. 2002;166:S9-S16.
27. Mehlen P, Kretz-Remy C, Briolay J, Fostan P, Mirault M, Arrigo A: Intracellular reactive oxygen species as apparent modulators of heat-shock protein 27 (Hsp27) structural organization and phosphorylation in basal and tumour necrosis factor α-treated T47D human carcinoma cells. Biochem J. 1995;312:367-375.
28. Venkatakrishnan C, Tewari A, Moldovan L, Cardounel A, Zweier J, Kuppusamy P, Ilangovan G: Heat shock protects cardiac cells from doxorubicin-induced toxicity by activating p38MAPK and phosphorylation of small heat shock protein 27. Am J Physiol (Heart Circ Physiol). 2006;291:H2680-H2691.
29. Powell C, Wright M, Jackson R: p38MAPK and MEK1/2 inhibition contribute to cellular oxidant injury after hypoxia. Am J Physiol (Lung Cell Mol Physiol). 2004;286:L826-L833.
30. Sun W, Huang Z, Lu S: Regulation of γ -glutamyl cysteine synthetase by protein phosphorylation. Biochem J. 1996;320:321-328.
31. Ochi T: Hydrogen peroxide increases the activity of γ - glutamylcysteine synthase in cultured Chinese hamster V79 cells. Arch Toxicol. 1995;70:96-103.
32. Zhang H, Court N, Forman H. Sub micromolar concentrations of 4-hydroxynonelal induce glutamate cysteine ligase expression in HBE 1 cells. Redox Rep. 2007;12:101-106.
33. KayyaliU, Pennella C, Trujillo C, Villa O, GaestelM,Hassoun P: Cytoskeletal changes in hypoxic pulmonary endothelial cells are dependent on MAPK-activated protein kinase MK2. J Biol Chem. 2002;277:42596-42602.
34. Jagavelu K, Tietge U, Gaestel M, Drexler H, Schieffer B, Bavendiek U: Systemic deficiency of the MAPK-activated protein kinase 2 reduces atherosclerosis in hypercholesterolemic mice. Circ Res. 2007;101:1104-1112.