Zymographic assay of plant diamine oxidase on entrapped peroxidase polyacrylamide gel electrophoresis. A study of stability to proteolysis

Analytical and Bioanalytical Chemistry

Volumn 396, Issue 3, 2010, Pages 1281-1290

  Calinescu, Carmen ^a   Federico, Rodolfo ^b   Mondovi, Bruno ^c   Mateescu, Mircea Alexandru ^a  

^a UNIVERSITÉ DU QUÉBEC À MONTRÉAL   (Canada)

^b ROMA TRE UNIVERSITY   (Italy)

^c SAPIENZA UNIVERSITY OF ROME   (Italy)

Author keywords

Diamine oxidase; Entrapped peroxidase polyacrylamide gel; Proteolytic stability; Simulated gastro intestinal conditions; Zymographic assay

Indexed keywords

AFTER-IMAGES; CATALYZED OXIDATION; CO-SUBSTRATE; CONCENTRATION OF; DIAMINE OXIDASE; ELECTROPHORETIC GELS; ENZYMATIC ACTIVITIES; GASTRIC CONDITIONS; HORSE-RADISH PEROXIDASE; INTESTINAL FLUID; LATHYRUS SATIVUS; LINEAR DEPENDENCY; ORTHO-PHENYLENEDIAMINE; PANCREATIC PROTEASE; POLYACRY LAMIDE; POLYACRYLAMIDE GEL ELECTROPHORESIS; POLYACRYLAMIDE GELS; PROTEIN BANDS; PROTEIN PATTERNS; PROTEOLYTIC STABILITY; SODIUM DODECYL SULFATE;

ELECTROPHORESIS; IMAGE ANALYSIS; PROTEINS; SODIUM; SODIUM SULFATE;

GELS;

AMINE OXIDASE (COPPER CONTAINING); PEROXIDASE;

ARTICLE; CHEMISTRY; ENZYMOLOGY; EVALUATION; ISOLATION AND PURIFICATION; LATHYRUS; METABOLISM; METHODOLOGY; PLANT SEED; POLYACRYLAMIDE GEL ELECTROPHORESIS; STOMACH JUICE;

AMINE OXIDASE (COPPER-CONTAINING); ELECTROPHORESIS, POLYACRYLAMIDE GEL; GASTROINTESTINAL CONTENTS; LATHYRUS; PEROXIDASES; SEEDS;

ARMORACIA RUSTICANA; LATHYRUS; LATHYRUS SATIVUS;

EID: 76749097620     PISSN: 16182642     EISSN: 16182650     Source Type: Journal    
DOI: 10.1007/s00216-009-3306-7     Document Type: Article

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