Molecular mechanism of polyethylene glycol mediated stabilization of protein

Biochemical and Biophysical Research Communications

Volumn 392, Issue 4, 2010, Pages 561-566

  Rawat, Sanjay ^a   Raman Suri, C ^a   Sahoo, Debendra K ^a  

^a INSTITUTE OF MICROBIAL TECHNOLOGY   (India)

Author keywords

BSA; Molecular mechanism; Polyethylene glycol; Protein stability

Indexed keywords

BOVINE SERUM ALBUMIN; MACROGOL;

ARTICLE; CIRCULAR DICHROISM; DIFFERENTIAL SCANNING CALORIMETRY; FLUORESCENCE SPECTROSCOPY; NONHUMAN; PRIORITY JOURNAL; PROTEIN BINDING; PROTEIN DENATURATION; PROTEIN STABILITY; THERMOSTABILITY;

ANIMALS; CATTLE; CIRCULAR DICHROISM; POLYETHYLENE GLYCOLS; PROTEIN BINDING; PROTEIN CONFORMATION; PROTEIN DENATURATION; PROTEIN STABILITY; SERUM ALBUMIN, BOVINE; SPECTROMETRY, FLUORESCENCE; TRYPTOPHAN; UREA;

BOVINAE;

EID: 76649145626     PISSN: 0006291X     EISSN: 10902104     Source Type: Journal    
DOI: 10.1016/j.bbrc.2010.01.067     Document Type: Article

References (21)

1. Ahmad B., Ansari M.A., Sen P., and Khan R.H. Low versus high molecular weight poly(ethylene glycol) induced states of stem bromelain at low pH: stabilization of molten globule and unfolded states. Biopolymers 81 (2006) 350-359
2. Simona L.M., Kotoraman M., Garab G., and Laczko I. Effects of polyhydroxy compounds on the structure and activity of α-chymotrypsin. Biochem. Biophys. Res. Commun. 293 (2002) 416-420
3. Annunziata O., Asherie N., Lomakin A., Pande J., Ogun O., and Benedek G.B. Effect of polyethylene glycol on the liquid-liquid phase transition in aqueous protein solutions. Proc. Natl. Acad. Sci. USA 99 (2002) 14165-14170
4. Hasek J. Poly(ethylene glycol) interactions with proteins. Z. Kristallogr. Suppl. 23 (2006) 613-618
5. Lundqvist M., Sethson I., and Jonsson B.H. Protein adsorption onto silica nanoparticles: conformational changes depend on the particles' curvature and the protein stability. Langmuir 20 (2004) 10639-10647
6. Carter D.C., and Ho J.X. Structure of serum albumin. Adv. Protein Chem. 45 (1994) 153-203
7. Wangoo N., Suri C.R., and Shekhawat G. Interaction of gold nanoparticles with protein: a spectroscopic study to monitor protein conformational changes. Appl. Phys. Lett. 92 (2008) 133104
8. Zhang M., Li X.H., Gong Y.D., Zhao N.M., and Zhang X.F. Properties and biocompatibility of chitosan films modified by blending with PEG. Biomaterials 23 (2002) 2641-2648
9. Schonbrunn E., Eschenburg S., Luger K., Kabsch W., and Amrhein N. Structural basis for the interaction of the fluorescence probe 8-anilino-1-naphthalene sulfonate (ANS) with the antibiotic target MurA. Proc. Natl. Acad. Sci. USA 97 (2000) 6345-6349
10. Farruggia B., Nerli B., Nuci H.D., Rigatusso R., and Pico G. Thermal features of the bovine serum albumin unfolding by polyethylene glycols. Int. J. Biol. Macromol. 26 (1999) 23-33
11. Sun C., Yang J., Wu X., Huang X., Wang F., and Liu S. Unfolding and refolding of bovine serum albumin by cetyl pyridinium bromide. Biophys. J. 88 (2005) 3518-3524
12. Cleland J.L., Builder S.E., Swartz J.R., Winkler M., Chang J.Y., and Wang D.I.C. Polyethylene glycol enhanced protein refolding. Biotechnology 10 (1992) 1013-1019
13. Dwyer J.J., Gittis A.G., Karp D.A., Lattman E.E., Spencer D.S., Stites W.E., and MorenoE B.G. High apparent dielectric constants in the interior of a protein reflect water penetration. Biophys. J. 79 (2000) 1610-1620
14. Rostovtseva T.K., Nestorovich E.M., and Bezrukov S.M. Partitioning of differently sized poly (ethylene glycol)s into ompF porin. Biophys. J. 82 (2002) 160-169
15. Movileanu L., Cheley S., and Bayley H. Partitioning of individual flexible polymers into a nanoscopic protein pore. Biophys. J. 85 (2003) 897-910
16. Bennion B.J., and Daggett V. The molecular basis for the chemical denaturation of proteins by urea. Proc. Natl. Acad. Sci. USA 100 (2003) 5142-5147
17. Zangi R., Zhou R., and Berne B.J. Urea's action on hydrophobic interactions. J. Am. Chem. Soc. 131 (2009) 1535-1541
18. Rocco A.G., Mollica L., Ricchiuto P., Baptista A.M., Gianazza E., and Eberini I. Characterization of the protein unfolding processes induced by urea and temperature. Biophys. J. 94 (2008) 2241-2251
19. Anna M. DSC study of association of ethanol with albumin. J. Therm. Anal. Calorim. 87 (2007) 91-97
20. Celej M.S., Montich G.G., and Fidelio G.D. Protein stability induced by ligand binding correlates with changes in protein flexibility. Protein Sci. 12 (2003) 1496-1506
21. Cleland J.L., and Randolph T.W. Mechanism of polyethylene glycol interaction with the molten globule folding intermediate of bovine carbonic anhydrase B. J. Biol. Chem. 267 (1992) 3147-3153