Cloning and functional expression of a nitrile hydratase (NHase) from Rhodococcus equi TG328-2 in Escherichia coli, its purification and biochemical characterisation

Applied Microbiology and Biotechnology

Volumn 85, Issue 5, 2010, Pages 1417-1425

  Rzeznicka, Kamila ^a,c   Schätzle, Sebastian ^a   Böttcher, Dominique ^a   Klein, Joachim ^b   Bornscheuer, Uwe T ^a  

^a UNIVERSITY OF GREIFSWALD   (Germany)

^b LONZA AG   (Switzerland)

^c c LEcta GmbH   (Germany)

Author keywords

Cloning; Nitrile hydratase; Nitriles; Recombinant expression; Rhodococcus equi

Indexed keywords

CELL EXTRACTS; CHARACTERISATION; E. COLI; FUNCTIONAL EXPRESSION; HIS-TAG; METHACRYLONITRILE; NITRILE HYDRATASES; PH OPTIMA; PURIFIED ENZYME; RECOMBINANT EXPRESSION; RHODOCOCCUS EQUI; SPECIFIC ACTIVITY; SUBUNIT GENES; THERMAL INACTIVATION;

AFFINITY CHROMATOGRAPHY; AMINO ACIDS; AROMATIC COMPOUNDS; CHROMATOGRAPHIC ANALYSIS; CLONING; ENANTIOSELECTIVITY; ENZYME ACTIVITY; ENZYMES; ESCHERICHIA COLI; GENE EXPRESSION; HYDRATES; ORGANIC ACIDS; PURIFICATION; ULTRAFILTRATION;

CYANIDES;

METHACRYLONITRILE; NITRILE; NITRILE HYDRATASE;

AMINO ACID; BACTERIUM; CLONE; ENZYME ACTIVITY; GENE; GENE EXPRESSION; RECOMBINATION; TEMPERATURE EFFECT; ULTRAFILTRATION;

AFFINITY CHROMATOGRAPHY; AMINO ACID ANALYSIS; ARTICLE; BACTERIAL GENE; CONTROLLED STUDY; ENANTIOSELECTIVITY; ENZYME ACTIVITY; ENZYME ASSAY; ENZYME INACTIVATION; ENZYME PURIFICATION; ENZYME SPECIFICITY; ENZYME STABILITY; ENZYME SYNTHESIS; ESCHERICHIA COLI; FREEZE DRYING; GAS CHROMATOGRAPHY; GENE EXPRESSION SYSTEM; GENE IDENTIFICATION; GENE SEQUENCE; HEAT TOLERANCE; HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; HYDRATION; INVERSE POLYMERASE CHAIN REACTION; MOLECULAR CLONING; NONHUMAN; PH; POLYACRYLAMIDE GEL ELECTROPHORESIS; PROMOTER REGION; RHODOCOCCUS EQUI; SEQUENCE ANALYSIS; ULTRAFILTRATION; WESTERN BLOTTING;

BACTERIAL PROTEINS; BASE SEQUENCE; CLONING, MOLECULAR; DNA, BACTERIAL; ELECTROPHORESIS, POLYACRYLAMIDE GEL; ENZYME STABILITY; ESCHERICHIA COLI; GENES, BACTERIAL; HYDRO-LYASES; HYDROGEN-ION CONCENTRATION; METHACRYLATES; NITRILES; PROMOTER REGIONS, GENETIC; RECOMBINANT PROTEINS; RHODOCOCCUS EQUI; SEQUENCE ANALYSIS, DNA; SPECTROPHOTOMETRY, ULTRAVIOLET; STEREOISOMERISM; SUBSTRATE SPECIFICITY; TEMPERATURE;

ESCHERICHIA COLI; RHODOCOCCUS EQUI;

EID: 76649141718     PISSN: 01757598     EISSN: None     Source Type: Journal    
DOI: 10.1007/s00253-009-2153-y     Document Type: Article

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