Efficient expression in E. coli of an enantioselective nitrile hydratase from Rhodococcus erythropolis

Biotechnology Letters

Volumn 30, Issue 4, 2008, Pages 755-762

  Song, Liya ^a,b,c   Yuan, Hong Jie ^c,d   Coffey, Lee ^c   Doran, John ^c   Wang, Mei Xiang ^d   Qian, Shijun ^a   O'Reilly, Catherine ^c  

^a INSTITUTE OF MICROBIOLOGY   (China)

^b UNIVERSITY OF CHINESE ACADEMY OF SCIENCES   (China)

^c South East Technological University   (Ireland)

^d INSTITUTE OF CHEMISTRY   (China)

Author keywords

Activator protein; Enantioselectivity; GroES GroEL; Nitrile hydratase; Rhodococcus erythropolis

Indexed keywords

ENANTIOSELECTIVITY; ENZYME ACTIVITY; ESCHERICHIA COLI; GENE EXPRESSION; PROTEINS;

ACTIVATOR PROTEINS; GROES GROEL; NITRILE HYDRATASE; RHODOCOCCUS ERYTHROPOLIS;

GENE ENCODING;

BACTERIAL PROTEIN; HYDROLYASE; NITRILE HYDRATASE; RECOMBINANT PROTEIN;

ARTICLE; CHEMISTRY; ENZYME SPECIFICITY; ENZYMOLOGY; ESCHERICHIA COLI; GENETICS; METABOLISM; RHODOCOCCUS; STEREOISOMERISM;

BACTERIAL PROTEINS; ESCHERICHIA COLI; HYDRO-LYASES; RECOMBINANT PROTEINS; RHODOCOCCUS; STEREOISOMERISM; SUBSTRATE SPECIFICITY;

ESCHERICHIA COLI; RHODOCOCCUS ERYTHROPOLIS;

EID: 43149097366     PISSN: 01415492     EISSN: 15736776     Source Type: Journal    
DOI: 10.1007/s10529-007-9611-3     Document Type: Article

References (22)

1. Blakey AJ, Colby J, Williams E, O'Reilly C (1995) Regio- and stereo-specific nitrile hydrolysis by the nitrile hydratase from Rhodococcus AJ270. FEMS Microbiol Lett 129:57-61
2. Endo I, Nojiri M, Tsujimura M, Nakasako M, Nagashima S, Yohda M, Odaka M (2001) Fe-type nitrile hydratase. J Inorg Biochem 83:247-253
3. Goda M, Hashimoto Y, Takase M, Herai S, Iwahara Y, Higashibata H, Kobayashi M (2002) Isonitrile hydratase from Pseudomonas putida N19-2. Cloning, sequencing, gene expression, and identification of its active acid residue. J Biol Chem 277:45860-45865
4. Hann EC, Eisenberg A, Fager SK, Perkins NE, Gallagher FG, Cooper SM, Gavagan JE, Stieglitz B, Hennessey SM, DiCosimo R (1999) 5-Cyanovaleramide production using immobilized Pseudomonas chlororaphis B23. Bioorg Med Chem 7:2239-2245
5. Hashimoto Y, Nishiyama M, Horinouchi S, Beppu T (1994) Nitrile hydratase gene from Rhodococcus sp. N-774 requirement for its downstream region for efficient expression. Biosci Biotechnol Biochem 58:1859-1865
6. Kobayashi M, Komeda H, Nagasawa T, Nishiyama M, Horinouchi S, Beppu T, Yamada H, Shimizu S (1993) Amidase coupled with low-molecular-mass nitrile hydratase from Rhodococcus rhodochrous J1. Sequencing and expression of the gene and purification and characterization of the gene product. Eur J Biochem 217:327-336
7. Kobayashi M, Nishiyama M, Nagasawa T, Horinouchi S, Beppu T, Yamada H (1991) Cloning, nucleotide sequence and expression in Escherichia coli of two cobalt-containing nitrile hydratase genes from Rhodococcus rhodochrous J1. Biochim Biophys Acta 1129:23-33
8. Kobayashi M, Shimizu S (2000) Nitrile hydrolases. Curr Opin Chem Biol 4:95-102
9. Komeda H, Kobayashi M, Shimizu S (1996) Characterization of the gene cluster of high-molecular-mass nitrile hydratase (H-NHase) induced by its reaction product in Rhodococcus rhodochrous J1. Proc Natl Acad Sci USA 93:4267-4272
10. Kopf MA, Bonnet D, Artaud I, Petre D, Mansuy D (1996) Key role of alkanoic acids on the spectral properties, activity, and active-site stability of iron-containing nitrile hydratase from Brevibacterium R312. Eur J Biochem 240:239-244
11. Loomis K, Rupp S, Yaeger K, Novy R, Wong S (2003) Enhanced protein coexpression in E. coli Novagen InNovations Newsl 18:7-12
12. Nagasawa T, Nanba H, Ryuno K, Takeuchi K, Yamada H (1987) Nitrile hydratase of Pseudomonas chlororaphis B23. Purification and characterization. Eur J Biochem 162:691-698
13. Nishihara K, Kanemori M, Kitagawa M, Yanagi K, Yura T (1998) Chaperone coexpression plasmids: differential and synergistic roles of DnaK-DnaJ-GrpE and GroEL-GroES in assisting folding of an allergen of Japanese Cedar Pollen, Cryj2, in Escherichia coli. Appl Environ Microbiol 64:1694-1699
14. Nishihara K, Kanemori M, Yanagi H, Yura T (2000) Overexpression of trigger factor prevents aggregation of recombinant proteins in Escherichia coli. Appl Environ Microbiol 66:884-889
15. Nishiyama M, Horinouchi S, Kobayashi M, Nagasawa T, Yamada H, Beppu T (1991) Cloning and characterization of genes responsible for metabolism of nitrile compounds from Pseudomonas chlororaphis B23. J Bacteriol 173:2465-2472
16. Nojiri M, Yohda M, Odaka M, Matsushita Y, Tsujimura M, Yoshida T, Dohmae N, Takio K, Endo I (1999) Functional expression of nitrile hydratase in Escherichia coli: requirement of a nitrile hydratase activator and post-translational modification of a ligand cysteine. J Biochem (Tokyo) 125:696-704
17. O'Mahony R, Doran J, Coffey L, Cahill OJ, Black GW, O'Reilly C (2005) Characterisation of the nitrile hydratase gene clusters of Rhodococcus erythropolis strains AJ270 and AJ300 and Microbacterium sp. AJ115 indicates horizontal gene transfer and reveals an insertion of IS1166. Antonie van Leeuwenhoek 87:221-232
18. Stevens JM, Rao SN, Jaouen M, Belghazi M, Schmitter JM, Mansuy D, Artaud I, Sari MA (2003) Chaperone-assisted expression, purification, and characterization of recombinant nitrile hydratase NI1 from Comamonas testosteroni. Protein Expr Purif 29:70-76
19. Thomas JG, Ayling A, Baneyx F (1997) Molecular chaperones, folding catalysts, and the recovery of active recombinant proteins from E. coli. Appl Biochem Biotechnol 66:197-238
20. Wang MX, Feng GQ (2002) A novel approach to enantiopure cyclopropane compounds from biotransformation of nitriles. New J Chem 26:1575-1583
21. Wang MX, Liu CS, Li JS (2002) Enzymatic desymmetrization of 3-alkyl- and 3-arylglutaronitriles, a simple and convenient approach to optically active 4-amino-3-phenylbutanoic acids. Tetrahedron Asymmetry 12:3367-3373
22. Wang MX, Liu CS, Li JS, Meth-Cohn O (2000) Microbial desymmetrization of 3-arylglutaronitriles, an unusual enhancement of enantioselectivity in the presence of additives. Tetrahedron Lett 41:8549-8552