Biochemical analysis of cellular target of S-trityl-l-cysteine derivatives using affinity matrix

Bioorganic and Medicinal Chemistry Letters

Volumn 20, Issue 5, 2010, Pages 1578-1580

  Shimizu, Makiko ^a   Ishii, Hirosuke ^a   Ogo, Naohisa ^a   Matsuno, Kenji ^a   Asai, Akira ^a  

^a UNIVERSITY OF SHIZUOKA   (Japan)

Author keywords

Anticancer; Biaryl; KSP; STLC

Indexed keywords

CARBOXYLIC ACID DERIVATIVE; CYSTEINE DERIVATIVE; KINESIN; PROTEIN KSP; UNCLASSIFIED DRUG;

AFFINITY CHROMATOGRAPHY; ANTINEOPLASTIC ACTIVITY; ARTICLE; CANCER CELL; CELL INTERACTION; CELL LYSATE; CHEMICAL ANALYSIS; CONTROLLED STUDY; CYTOPLASM; CYTOTOXICITY; DRUG DESIGN; DRUG SYNTHESIS; HUMAN; HUMAN CELL; IMMOBILIZED CELL; STRUCTURE ACTIVITY RELATION;

ANTINEOPLASTIC AGENTS; CELL LINE, TUMOR; CYSTEINE; ENZYME INHIBITORS; HUMANS; IMMOBILIZED PROTEINS; KINESIN; PROTEIN BINDING;

EID: 76649102075     PISSN: 0960894X     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.bmcl.2010.01.088     Document Type: Article

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13. +.
14. 2O (1 mL, three times), 1 mmol/L ethanolamine (2 mL), 50 mmol/L Tris buffer (1 mL, pH 8), and 70 mmol/L AcOH (1 mL, pH 4). Tris buffer and AcOH washings were repeated three times and the resin was washed with ultrapure water (2 mL, three times), and stored in ultrapure water/ethanol (4/1, v/v) until use. For the control beads, a solution of ethanolamine in DMSO (100 mmol/L, 140 μL) was used instead of the solution of 3c in DMSO.
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