Inhibition of neuropilin-1 by RNA-interference and its angiostatic potential in the treatment of hepato-cellular carcinoma

Zeitschrift fur Gastroenterologie

Volumn 48, Issue 1, 2010, Pages 21-27

  Raskopf, E ^a   Vogt, A ^a   Standop, J ^a   Sauerbruch, T ^a   Schmitz, V ^a  

^a UNIVERSITY OF BONN   (Germany)

Author keywords

Anti endothelial effects; Hepatocellular carcinoma; Neuropilin 1; RNA interference; VEGF signaling

Indexed keywords

MITOGEN ACTIVATED PROTEIN KINASE 1; MITOGEN ACTIVATED PROTEIN KINASE 14; MITOGEN ACTIVATED PROTEIN KINASE 3; NEUROPILIN 1; PROTEIN KINASE B; SMALL INTERFERING RNA; VASCULAR CELL ADHESION MOLECULE 1; VASCULOTROPIN;

ANTIANGIOGENIC ACTIVITY; APOPTOSIS; ARTICLE; ENDOTHELIUM CELL; GENE EXPRESSION; HUMAN; HUMAN CELL; IMMUNOHISTOCHEMISTRY; LIVER CELL CARCINOMA; MICROVASCULATURE; PROTEIN EXPRESSION; RNA INTERFERENCE; SIGNAL TRANSDUCTION;

ANGIOGENESIS INHIBITORS; ANIMALS; APOPTOSIS; CELL LINE, TUMOR; CELL PROLIFERATION; ENDOTHELIUM, VASCULAR; GENE EXPRESSION REGULATION; GENE KNOCKDOWN TECHNIQUES; HUMANS; LIVER NEOPLASMS, EXPERIMENTAL; MALE; MICE; MICE, INBRED C3H; MICROCIRCULATION; NEOPLASM TRANSPLANTATION; NEUROPILIN-1; RNA, SMALL INTERFERING; SIGNAL TRANSDUCTION; TRANSFECTION; VASCULAR ENDOTHELIAL GROWTH FACTOR A;

EID: 76549103146     PISSN: 00442771     EISSN: None     Source Type: Journal    
DOI: 10.1055/s-0028-1109907     Document Type: Article

References (17)

1. Soker S, Takashima S, Miao HQ et al. Neuropilin-1 is expressed by endothelial and tumor cells as an isoform-specific receptor for vascular endothelial growth factor. Cell 1998; 92: 735-745
2. Guttmann-Raviv N, Kessler O, Shraga-Heled N et al. The neuropilins and their role in tumorigenesis and tumor progression. Cancer Lett 2006; 231: 1-11
3. Robinson C], Stringer SE. The splice variants of vascular endothelial growth factor (VEGF) and their receptors. J Cell Sci 2001; 114 (Pt 5): 853-865
4. Soker S. Neuropilin in the midst of cell migration and retraction. Int J Biochem Cell Biol 2001; 33: 433-437
5. Pan Q, Chanthery Y, Liang WC et al. Blocking neuropilin-1 function has an additive effect with anti-VEGF to inhibit tumor growth. Cancer Cell 2007; 11: 53-67
6. Miao HQ, Lee P, Lin H et al. Neuropilin-1 expression by tumor cells promotes tumor angiogenesis and progression. Faseb J 2000; 14: 2532-2539
7. Quante M, Raskopf E, Stahl S et al. No functional and transductional significance of specific neuropilin 1 siRNA inhibition in colon carcinoma cell lines lacking VEGF receptor 2. Oncol Rep 2009; 21: 1161-1168
8. Llovet JM, Bruix J. Novel advancements in the management of hepato-cellular carcinoma in 2008. J Hepatol 2008; 48 (Suppl 1): S20-S37
9. Llovet JM, Bruix J. Molecular targeted therapies in hepatocellular carcinoma. Hepatology 2008; 48: 1312-1327
10. Raskopf E, Vogt A, Sauerbruch T et al. siRNA targeting VEGF inhibits hepatocellular carcinoma growth and tumor angiogenesis in vivo. J Hepatol 2008; 49: 977-984
11. Kornek M, Lukacs-Kornek V, Limmer A et al. DOTAP formulated (immune-stimulatory) VEGF-A siRNA increased antitumoral efficacy in orthotopic HCC in murine liver fibrosis. Mol Med 2008; 14 (7-8): 365-373
12. Mosmann T. Rapid colorimetric assay for cellular growth and survival: application to proliferation and cytotoxicity assays. J Immunol Methods 1983; 65: 55-63
13. Pan Q, Chathery Y, Wu Y et al. Neuropilin-1 binds to VEGF121 and regulates endothelial cell migration and sprouting. J Biol Chem 2007; 282: 24049-24056
14. Schmitz V, Raskopf E, Gonzalez-Carmona MA et al. Plasminogen fragment K1-5 improves survival in a murine hepatocellular carcinoma model. Gut 2007; 56: 271-278
15. Zachary I. VEGF signalling: integration and multi-tasking in endothelial cell biology. Biochem Soc Trans 2003; 31 (Pt 6): 1171-1177
16. Rajan S, Ye J, Bai S et al. NF-kappaB, but not p38 MAP kinase, is required for TNF-alpha-induced expression of cell adhesion molecules in endothelial cells. J Cell Biochem 2008; 105: 477-486
17. Robbins M, Judge A, Ambegia E et al. Misinterpreting the therapeutic effects of siRNA caused by immune stimulation. Hum Gene Ther 2008; 19 (10): 991-999