The severe acute respiratory syndrome coronavirus Nsp15 protein is an endoribonuclease that prefers manganese as a cofactor

Journal of Virology

Volumn 78, Issue 22, 2004, Pages 12218-12224

  Bhardwaj, Kanchan ^a   Guarino, Linda ^a   Kao, C Cheng ^a  

^a TEXAS A AND M UNIVERSITY   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

DOUBLE STRANDED RNA; MAGNESIUM; MANGANESE; METAL ION; OLIGONUCLEOTIDE; RECOMBINANT PROTEIN; RIBONUCLEASE; RNA POLYMERASE; TRYPTOPHAN; URIDINE PHOSPHATE; VIRUS PROTEIN;

AMINO ACID SEQUENCE; AMINO TERMINAL SEQUENCE; ARTICLE; CARBOXY TERMINAL SEQUENCE; CONTROLLED STUDY; ENZYME ACTIVITY; ENZYME ANALYSIS; ENZYME KINETICS; ENZYME SPECIFICITY; ESCHERICHIA COLI; FLUORESCENCE; NONHUMAN; PRIORITY JOURNAL; PROTEIN CONFORMATION; PROTEIN EXPRESSION; PROTEIN STABILITY; RNA CLEAVAGE; RNA SYNTHESIS; SARS CORONAVIRUS; SEQUENCE ALIGNMENT;

AMINO ACID SEQUENCE; ENDORIBONUCLEASES; MANGANESE; MOLECULAR SEQUENCE DATA; SARS VIRUS; VIRAL NONSTRUCTURAL PROTEINS;

EID: 7644238616     PISSN: 0022538X     EISSN: None     Source Type: Journal    
DOI: 10.1128/JVI.78.22.12218-12224.2004     Document Type: Article

References (33)

1. pro) structure: basis for design of anti-SARS drugs. Science 300:1763-1767.
2. Baker, S. C., and M. M. Lai. 1990. An in vitro system for the leader-primed transcription of coronavirus mRNAs. EMBO J. 9:4173-4179.
3. 2+-dependent ribonuclease that functions in U16 SnoRNA processing in X. laevis. Biochem. Biophys. Res. Commun. 233:514-517.
4. Enserink, M. 2003. Clues to the animal origins of SARS. Science 300:1351.
5. 2+ ions reveals two divalent metals bound in the active site. J. Biol. Chem. 276:7266-7271.
6. Ivanov, K. A., V. Theil, J. C. Dobbe, Y. van der Meer, E. J. Snijder, and J. Ziebuhr. 2004. Multiple activities associated with severe acute respiratory syndrome coronavirus helicase. J. Virol. 78:5619-5632.
7. Joo, M., and S. Makino. 1992. Mutagenic analysis of the coronavirus intergenic consensus sequence. J. Virol. 66:6330-6337.
8. Kao, C., M. Zheng, and S. Rudisser. 1999. A simple and efficient method to reduce nontemplated nucleotide addition at the 3′ terminus of RNAs transcribed by T7 RNA polymerase. RNA 5:1268-1272.
9. Kao, C. C., and J. H. Sun. 1996. Initiation of minus-strand RNA synthesis by brome mosaic virus RNA-dependent RNA polymerase: use of oligoribonucleotide primers. J. Virol. 70:6826-6830.
10. Keck, J. L., E. R. Goedken, and S. Marqusee. 1998. Activation/attenuation model for RNase H. A one-metal mechanism with second-metal inhibition. J. Biol. Chem. 273:34128-34133.
11. Kim, C. H., C. C. Kao, and I. Tinoco. 2000. RNA motifs that determine specificity between a viral replicase and its promoter. Nat. Struct. Biol. 7:415-423.
12. Kim, Y.-C., G. Roberts, M. Thomson, and C. C. Kao. Spectroscopic analysis of the hepatitis C virus RNA polymerase. Submitted for publication.
13. Lai, M. M. C., and D. Cavanagh. 1997. The molecular biology of coronaviruses. Adv. Virus Res. 48:1-100.
14. Laneve, P., F. Altieri, M. E. Fiori, A. Scaloni, I. Bozzoni, and E. Caffarelli. 2003. Purification, cloning, and characterization of XendoU, a novel endoribonuclease involved in processing of intron-encoded small nucleolar RNAs in Xenopus laevis. J. Biol. Chem. 278:13026-13032.
15. Peiris, J. S., C. M. Chu, V. C. Cheng, K. S. Chan, I. F. Hung, L. L. Poon, K. I. Law, B. S. Tang, T. Y. Hon, C. S. Chan, K. H. Chan, J. S. Ng, B. J. Zheng, W. L. Ng, R. W. Lai, Y. Guan, and K. Y. Yuen. 2003. Clinical progression and viral load in a community outbreak of coronavirus-associated SARS pneumonia: a prospective study. Lancet 361:1767-1772.
16. Poutanen, S. M., D. E. Low, B. Henry, S. Finkelstein, D. Rose, K. Green, R. Tellier, R. Draker, D. Adachi, M. Ayers, A. K. Chan, D. M. Skowronski, I. Salit, A. E. Simor, A. S. Slutsky, P. W. Doyle, M. Krajden, M. Petric, R. C. Brunham, and A. J. McGeer. 2003. Identification of severe acute respiratory syndrome in Canada. N. Engl. J. Med. 348:1995-2005.
17. Ranjith-Kumar, C. T., J. Gajewski, L. Gutshall, D. Maley, R. T. Sarisky, and C. C. Kao. 2001. Terminal nucleotidyl transferase activity of recombinant Flaviviridae RNA-dependent RNA polymerases: implication for viral RNA synthesis. J. Virol. 75:8615-8623.
18. Ranjith-Kumar, C. T., Y. C. Kim, L. Gutshall, C. Silverman, S. Khandekar, R. T. Sarisky, and C. C. Kao. 2002. Mechanism of de novo initiation of RNA synthesis by the hepatitis C virus RNA-dependent RNA polymerase: role of divalent metals. J. Virol. 76:12513-12525.
19. Ranjith-Kumar, C. T., X. Zhang, and C. C. Kao. 2003. Enhancer-like activity of a brome mosaic virus RNA promoter J. Virol. 77:1830-1839.
20. Rao, P., W. Yuan, and R. M. Krug. 2003. Crucial role of CA cleavage sites in the cap-snatching mechanism for initiating viral mRNA synthesis. EMBO J. 22:1188-1198.
21. Rost, B. 1996. PHD: predicting one-dimensional protein structure by profile based neural networks. Methods Enzymol. 266:525-539.
22. Rota, P. A., M. S. Oberste, S. S. Monroe, W. A. Nix, R. Campagnoli, J. P. Icenogle, S. Penaranda, B. Bankamp, K. Maher, M. H. Chen, S. Tong, A. Tamin, L. Lowe, M. Frace, J. L. DeRisi, Q. Chen, D. Wang, D. D. Erdman, T. C. Peret, C. Burns, T. G. Ksiazek, P. E. Rollin, A. Sanchez, S. Liffick, B. Holloway, J. Limor, K. McCaustland, M. Olsen-Rasmussen, R. Fouchier, S. Gunther, A. D. Osterhaus, C. Drosten, M. A. Pallansch, L. J. Andersno, and W. J. Bellini. 2003. Characterization of a novel coronavirus associated with severe acute respiratory syndrome. Science 300:1394-1399.
23. Sawicki, S. G., and D. L. Sawicki. 1990. Coronavirus transcription: subgenomic mouse hepatitis virus replicative intermediates function in RNA synthesis. J. Virol. 64:1050-1056.
24. Sawicki, S. G., and D. L. Sawicki. 1998. A new model for coronavirus transcription. Adv. Exp. Med. Biol. 440:215-219.
25. Snijder, E. J., P. J. Bredenbeek, J. C. Dobbe, V. Thiel, J. Ziebuhr, L. L. Poon, Y. Guan, M. Rozanov, W. J. Spaan, and A. E. Gorbalenya. 2003. Unique and conserved features of genome and proteome of SARS-coronavirus, an early split-off from the coronavirus group 2 lineage. J. Mol. Biol. 331:991-1004.
26. Spaan, W., D. Cavanagh, and M. C. Horzinek. 1998. Coronaviruses: structure and genome expression. J. Gen. Virol. 69:2939-2952.
27. Sutton, G., E. Fry, L. Carter, S. Sainsbury, T. Walter, J. Nettleship, N. Berrow, R. Owens, R. Gilbert, A. Davidson, S. Siddell, L. L. Poon, J. Diprose, D. Alderton, M. Walsh, J. M. Grimes, and D. I. Stuart. 2004. The nsp9 replicase protein of SARS-coronavirus, structure and functional insights. Structure 12:341-353.
28. Thompson, J. D., D. G. Biggins, and T. J. Gibson. 1994. CLUSTAL W: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice. Nucleic Acids Res. 22:4673-4680.
29. van der Most, R. G., R. J. de Groot, and W. J. Spaan. 1994. Subgenomic RNA synthesis directed by a synthetic defective interfering RNA of mouse hepatitis virus: a study of coronavirus transcription initiation. J. Virol. 68: 3656-3666.
30. van Marie, G., J. C. Dobbe, A. P. Gultyaev, W. Luytjes, W. J. Spaan, and E. J. Snijder. 1999. Arterivirus discontinuous mRNA transcription is guided by base pairing between sense and antisense transcription-regulating sequences. Proc. Natl. Acad. Sci. USA 96:12056-12061.
31. World Health Organization. 15 August 2003, posting date. Summary table of SARS cases by country, 1 November 2002-7 August 2003. [Online.] http://www.who.int/csr/sars/country/en/country2003_08_15.pdf.
32. Ziebuhr, J., E. J. Snijder, and A. E. Gorbalenya. 2002. Virus-encoded proteinases and proteolytic processing in the Nidovirales. J. Gen. Virol. 81:853-579.
33. Zuker, M., D. H. Mathews, and D. H. Turner. 1999. Algorithms and thermodynamics for RNA secondary structure prediction: a practical guide, p. 11-43. In J. Barciszewski and B. F. C. Clark (ed.), RNA biochemistry and biotechnology. NATO ASI Series, Kluwer Academic Publishers, Dordrecht, The Netherlands.