Simultaneous Detection of Marine Fish Pathogens by Using Multiplex PCR and a DNA Microarray

Journal of Clinical Microbiology

Volumn 42, Issue 4, 2004, Pages 1414-1419

  González, Santiago F ^a,c   Krug, Melissa J ^b   Nielsen, Michael E ^c   Santos, Ysabel ^a   Call, Douglas R ^b  

^a UNIVERSITY OF SANTIAGO DE COMPOSTELA   (Spain)

^b WASHINGTON STATE UNIVERSITY   (United States)

^c UNIVERSITY OF COPENHAGEN   (Denmark)

Author keywords

[No Author keywords available]

Indexed keywords

ARTICLE; CHROMOSOMAL LOCALIZATION; DNA MICROARRAY; DNA NICK TRANSLATION; ENZYME LINKED IMMUNOSORBENT ASSAY; FISH DISEASE; FURUNCULOSIS; GENE TARGETING; MARINE ENVIRONMENT; MICROORGANISM DETECTION; NONHUMAN; NUCLEOTIDE SEQUENCE; OLIGONUCLEOTIDE PROBE; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; RNA GENE; STRAIN DIFFERENCE;

ANIMALS; DNA, BACTERIAL; FISH DISEASES; FISHES; GRAM-NEGATIVE BACTERIA; GRAM-NEGATIVE BACTERIAL INFECTIONS; OLIGONUCLEOTIDE ARRAY SEQUENCE ANALYSIS; POLYMERASE CHAIN REACTION; SEAWATER; SENSITIVITY AND SPECIFICITY;

AEROMONAS; AEROMONAS SALMONICIDA; AEROMONAS SALMONICIDA SUBSP. SALMONICIDA; LISTONELLA; LISTONELLA ANGUILLARUM; NEGIBACTERIA; PHOTOBACTERIUM; PHOTOBACTERIUM DAMSELAE; PHOTOBACTERIUM DAMSELAE SUBSP. DAMSELAE; VIBRIO; VIBRIO PARAHAEMOLYTICUS; VIBRIO VULNIFICUS;

EID: 7644231947     PISSN: 00951137     EISSN: None     Source Type: Journal    
DOI: 10.1128/JCM.42.4.1414-1419.2004     Document Type: Article

References (44)

1. Agresti, A., and B. Coull. 1998. Approximate is better than 'exact' for interval estimation of binomial proportions. Am. Stat. 52:119-126.
2. Arias, C. R., E. Garay, and R. Aznar. 1995. Nested PCR method for rapid and sensitive detection of Vibrio vulnificus in fish, sediments, and water. Appl. Environ. Microbiol. 61:3476-3478.
3. Austin, B., and D. Austin. 1999. Bacterial fish pathogens: disease of farmed and wild fish, 3rd ed. Springer-Praxis, London, England.
4. Brasher, C. W., A. DePaola, D. D. Jones, and A. K. Bej. 1998. Detection of microbial pathogens in shellfish with multiplex PCR. Curr. Microbiol. 37:101-107.
5. Call, D., M. Borucki, and T. Besser. 2003. Mixed-genome microarrays reveal multiple serotype and lineage-specific differences among strains of Listeria monocytogenes. J. Clin. Microbiol. 41:632-639.
6. Call, D., D. Chandler, and F. Brockman. 2001. Fabrication of DNA microarrays using unmodified oligonucleotide probes. BioTechniques 30:368-379.
7. Call, D. R., M. Bakko, M. Krug, and M. Roberts. 2003. Identifying antimicrobial resistance genes using DNA microarrays. Antimicrob. Agents Chemother. 47:3290-3295.
8. Call, D. R., M. Borucki, and F. Loge. 2003. Detection of bacterial pathogens in environmental samples using DNA microarrays. J. Microbiol. Methods 53:235-243.
9. Call, D. R., F. J. Brockman, and D. P. Chandler. 2001. Detecting and genotyping Escherichia coli O157:H7 using multiplexed PCR and nucleic acid microarrays. Int. J. Food Microbiol. 67:71-80.
10. Chizhikov, V., A. Rasooly, K. Chumakov, and D. Levy. 2001. Microarray analysis of microbial virulence factors. Appl. Environ. Microbiol. 67:3258-3263.
11. Chizhikov, V., M. Wagner, A. Ivshina, Y. Hoshino, A. Z. Kapikian, and K. Chumakov. 2002. Detection and genotyping of human group A rotaviruses by oligonucleotide microarray hybridization. J. Clin. Microbiol. 40:2398-2407.
12. Cho, J. C., and J. M. Tiedje. 2001. Bacterial species determination from DNA-DNA hybridization by using genome fragments and DNA microarrays. Appl. Environ. Microbiol. 67:3677-3682.
13. Chu, S., S. Cavaignac, J. Feutrier, B. M. Phipps, M. Kostrzynska, W. W. Kay, and T. J. Trust. 1991. Structure of the tetragonal surface virulence array protein and gene of Aeromonas salmonicida. J. Biol. Chem. 266:15258-15265.
14. Del Cerro, A., I. Marquez, and J. A. Guijarro. 2002. Simultaneous detection of Aeromonas salmonicida, Flavobacterium psychrophilum, and Yersinia ruckeri, three major fish pathogens, by multiplex PCR. Appl. Environ. Microbiol. 68:5177-5180.
15. Gibson, G. 2002. Microarrays in ecology and evolution: a preview. Mol. Ecol. 11:17-24.
16. González, S., C. R. Osorio, and Y. Santos. 2003. Development of a PCR-based method for the detection of Listonella anguillarum in fish tissues and blood samples. Dis. Aquat. Org. 55:109-115.
17. Guschin, D. Y., B. K. Mobarry, D. Proudnikov, D. A. Stahl, B. E. Rittmann, and A. D. Mirzabekov. 1997. Oligonucleotide microchips as genosensors for determinative and environmental studies in microbiology. Appl. Environ. Microbiol. 63:2397-2402.
18. Hiney, M., M. T. Dawson, D. M. Heery, P. R. Smith, F. Gannon, and R. Powell. 1992. DNA probe for Aeromonas salmonicida. Appl. Environ. Microbiol. 58:1039-1042.
19. Hoie, S., M. Heum, and O. Thorensen. 1997. Evaluation of a polymerase chain reaction-based assay for the detection of Aeromonas salmonicida subsp. salmonicida in Atlantic salmon, Salmo salar. Dis. Aquat. Org. 30:27-35.
20. Kimura, B., S. Hokimoto, H. Takahashi, and T. Fujii. 2000. Photobacterium histaminum Okuzumi et al. 1994 is a later subjective synonym of Photobacterium damselae subsp. damselae (Love et al. 1981) Smith et al. 1991. Int. J. Syst. Evol. Microbiol. 50:1339-1342.
21. Kita-Tsukamoto, K., H. Oyaizu, K. Nanba, and U. Simidu. 1993. Phylogenetic relationships of marine bacteria, mainly members of the family Vibrionaceae, determined on the basis of 16S rRNA sequences. Int. J. Syst. Bacteriol. 43:8-19.
22. Klontz, K. C., S. Lieb, M. Schreiber, H. T. Janowski, L. M. Baldy, and R. A. Gunn. 1988. Syndromes of Vibrio vulnificus infections. Clinical and epidemiologic features in Florida cases, 1981-1987. Ann. Intern. Med. 109:318-323.
23. Loge, F. J., D. E. Thompson, and D. R. Call. 2002. PCR detection of specific pathogens in water: a risk-based analysis. Environ. Sci. Technol. 36:2754-2759.
24. Magnusson, H. B., O. H. Fridjonsson, O. S. Andresson, E. Benediktsdottir, S. Gudmundsdottir, and V. Andresdottir. 1994. Renibacterium salmoninarum, the causative agent of bacterial kidney disease in salmonid fish, detected by nested reverse transcription-PCR of 16S rRNA sequences. Appl. Environ. Microbiol. 60:4580-4583.
25. Marshall, S., S. Heath, V. Henriquez, and C. Orrego. 1998. Minimally invasive detection of Piscirickettsia salmonis in cultivated salmonids via the PCR. Appl. Environ. Microbiol. 64:3066-3069.
26. McNamara, P. J., W. A. Cuevas, and J. G. Songer. 1995. Toxic phospholipases D of Corynebacterium pseudotuberculosis, C. ulcerans and Arcanobacterium haemolyticum: cloning and sequence homology. Gene 156:113-118.
27. Miller, V. L., R. K. Taylor, and J. J. Mekalanos. 1987. Cholera toxin transcriptional activator ToxR is a transmembrane DNA binding protein. Cell 48:271-279.
28. Mooney, J., E. Powell, C. Clabby, and R. Powell. 1995. Detection of Aeromonas salmonicida in wild Atlantic salmon using a specific DNA probe test. Dis. Aquat. Org. 21:131-135.
29. Nakashima, S., and K. Takimoto. 1987. The epidemiological data of food poisoning in 1986. Food Sanit. Res. 37:50-76.
30. Osorlo, C., and A. Toranzo. 2002. DNA-based diagnostics in sea farming, p. 253-310. In M. Fingerman and R. Nagabhushanam (ed.), Seafood safety and human health. Science Publishers, Inc., Enfield, N.H.
31. Osorio, C. R., M. D. Collins, A. E. Toranzo, J. L. Barja, and J. L. Romalde. 1999. 16S rRNA gene sequence analysis of Photobacterium damselae and nested PCR method for rapid detection of the causative agent of fish pasteurellosis. Appl. Environ. Microbiol. 65:2942-2946.
32. Osorio, C. R., A. E. Toranzo, J. L. Romalde, and J. L. Barja. 2000. Multiplexed PCR assay for ureC and 16S rRNA genes clearly discriminates between both subspecies of Photobacterium damselae. Dis. Aquat. Org. 40:177-183.
33. O'Toole, R., D. L. Milton, P. Horstedt, and H. Wolf-Watz. 1997. rpoN of the fish pathogen Vibrio (Listonella) anguillarum is essential for flagellum production and virulence by the water-borne but not intraperitoneal route of inoculation. Microbiology 143:3849-3859.
34. Pazos, F., Y. Santos, Macías, S. Núñez, and A. E. Toranzo. 1996. Evaluation of media for the successful culture of Flexibacter maritimus. J. Fish Dis. 19:193-197.
35. Pedersen, K., B. Austin, D. A. Austin, and J. L. Larsen. 1999. Vibrios associated with mortality in cultured plaice Pleuronectes platessa fry. Acta Vet. Scand. 40:263-270.
36. Rozen, S., and H. Skaletsky. 2000. Primer3 on the WWW for general users and for biologist programmers, p. 365-386. In S. Krawetz and S. Misener (ed.), Bioinformatics methods and protocols: methods in molecular biology. Humana Press, Totowa, N.J.
37. Ruimy, R., V. Breittmayer, P. Elbaze, B. Lafay, O. Boussemart, M. Gauthier, and R. Christen. 1994. Phylogenetic analysis and assessment of the genera Vibrio, Photobacterium, Aeromonas, and Plesiomonas deduced from small-subunit rRNA sequences. Int. J. Syst. Bacteriol. 44:416-426.
38. Schena, M. 2000. Microarray biochip technology. Eaton Publishing, Natick, Mass.
39. Tolmasky, M. E., L. A. Actis, and J. H. Crosa. 1993. A single amino acid change in AngR, a protein encoded by pJM1-like virulence plasmids, results in hyperproduction of anguibactin. Infect. Immun. 61:3228-3233.
40. Toranzo, A. E., and J. L. Barja. 1993. Fry mortality syndrome (FMS) in Spain. Isolation of the causative Flexibacter psychrophilus. Bull. Eur. Assoc. Fish Pathol. 13:30-32.
41. Toranzo, A. E., Y. Santos, S. Nuñez, and J. Barja. 1991. Biochemical and serological characteristics, drug resistance and plasmid profiles of Spanish isolates of Aeromonas salmonicida. Gyobyo Kenkyu 26:55-60.
42. Venkateswaran, K., N. Dohmoto, and S. Harayama. 1998. Cloning and nucleotide sequence of the gyrB gene of Vibrio parahaemolyticus and its application in detection of this pathogen in shrimp. Appl. Environ. Microbiol. 64:681-687.
43. Volokhov, D., A. Rasooly, K. Chumakov, and V. Chizhikov. 2002. Identification of Listeria species by microarray-based assay. J. Clin. Microbiol. 4:4720-4728.
44. Yamamoto, K., A. C. Wright, J. B. Kaper, and J. G. Morris. 1990. The cytolysin gene of Vibrio vulnificus: sequence and relationship to the Vibrio cholerae El Tor hemolysin gene. Infect. Immun. 58:2706-2709.