Purification and biological characterization of a halophilic thermostable protease from Haloferax lucentensis VKMM 007

World Journal of Microbiology and Biotechnology

Volumn 25, Issue 12, 2009, Pages 2247-2256

  Manikandan, Muthu ^a   Pašić, Lejla ^b   Kannan, Vijayaraghavan ^a  

^a UNIVERSITY OF MADRAS   (India)

^b UNIVERSITY OF LJUBLJANA   (Slovenia)

Author keywords

Archaea; Characterization; Haloferax lucentensis; Halophile; Halophilic protease; Purification

Indexed keywords

ARCHAEA; ARCHAEON; BIOLOGICAL CHARACTERIZATION; DEGREE OF SPECIFICITY; ENZYME SUBSTRATES; EXTRACELLULAR ENZYMES; GEL FILTRATION; NACL CONCENTRATION; NON-POLAR SOLVENTS; POLYPEPTIDE CHAIN; PROTEOLYTIC ACTIVITIES; PURIFIED PROTEIN; SDS-PAGE; SEPHADEX; TRYPSIN-LIKE SERINE PROTEASE;

AFFINITY CHROMATOGRAPHY; AMINO ACIDS; CALCIUM; CHROMATOGRAPHIC ANALYSIS; ENZYMES; GEL PERMEATION CHROMATOGRAPHY; ION EXCHANGE; PHOSPHATASES; REDUCING AGENTS; SODIUM CHLORIDE; SURFACE ACTIVE AGENTS; ULTRAFILTRATION;

PURIFICATION;

ARCHAEA; HALOFERAX; HALOFERAX LUCENTENSE;

EID: 76349122463     PISSN: 09593993     EISSN: None     Source Type: Journal    
DOI: 10.1007/s11274-009-0132-1     Document Type: Article

References (37)

1. Akolkar AV, Deshpande GM, Raval KN, Durai D, Nerurkar AS, Desai AJ (2008) Organic solvent tolerance of Halobacterium sp. SP1(1) and its extracellular protease. J Basic Microbiol 48: 421-425.
2. Blum H, Beier H, Gross HJ (1987) Improved silver staining of plant proteins, RNA and DNA in polyacrylamide gels. Electrophoresis 8: 93-99.
3. Bohm G, Jaenicke R (1994) Relevance of sequence statistics for the properties of extremophilic proteins. Int J Pept Protein Res 43: 97-106.
4. Bradford MM (1976) A rapid and sensitive method for quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem 72: 248-254.
5. De Castro RE, Maupin-Furlow JA, Gimenez M, Seitz MH, Sanchez JJ (2006) Haloarchaeal proteases and proteolytic systems. FEMS Microbiol Rev 30: 17-35.
6. Ghorbel B, Kamoun AS, Nasri M (2003) Stability studies of protease from Bacillus cereus BG1. Enzyme Microb Tech 32: 513-518.
7. Giménez MI, Studdert CA, Sánchez JJ, De Castro RE (2000) Extracellular protease of Natrialba magadii: purification and biochemical characterization. Extremophiles 4: 181-188.
8. Gutierrez MC, Kamekura M, Holmes ML, Dyall-Smith ML, Ventosa A (2002) Taxonomic characterization of Haloferax sp. ("H. alicantei") strain Aa 2. 2: description of Haloferax lucentensis sp. nov. Extremophiles 6: 479-483.
9. Izotova LS, Strongin AY, Chekulaeva LW, Sterkin VE, Ostoslavskaya VI, Lyublinskaya EA, Timokhina EA, Stepanov VM (1983) Purification and properties of serine protease from Halobacterium halobium. J Bacteriol 155: 826-830.
10. Jimenez CR, Huang L, Qiu Y, Burlingame AL (1998) Sample preparations for MALDI mass analysis of peptides and proteins. In: Coligan JE, Dunn BM, Ploogh HL, Speicher DW, Wingfield PT (eds) Current protocols in protein science, vol 1. Wiley, NY.
11. Joo WA, Kim CW (2005) Proteomics of halophilic archaea. J Chromatogr B Analyt Technol Biomed Life Sci 815: 237-250.
12. Kamekura M, Seno Y (1990) A halophilic extracellular proteasefrom a halophilic archaebacterium strain 172P1. Biochem Cell Biol 68: 352-359.
13. Kamekura M, Seno Y, Holmes ML, Dyall-Smith ML (1992) Molecular cloning and sequencing of the gene for a halophilic alkaline serine protease (halolysin) from an unidentified halophilic archaea strain (172P1) and expression of the gene in Haloferax volcanii. J Bacteriol 174: 736-742.
14. Kamekura M, Seno Y, Dyall-Smith ML (1996) Halolysin R4, a serine proteinase from the halophilic archaeon Haloferax mediterranei; gene cloning, expression and structural studies. Biochim Biophys Acta 1294: 159-167.
15. Klibanov AM (2001) Improving enzymes by using them in organic solvents. Nature 409: 241-246.
16. Kim J, Dordick JS (1997) Unusual salt and solvent dependence of a protease from an extreme halophile. Biotechnol Bioeng 55: 471-479.
17. Lanyi JK (1974) Salt-dependent properties of proteins from extremely halophilic bacteria. Bacteriol Rev 38: 272-290.
18. Madern D, Ebel C, Zaccai G (2000) Halophilic adaptation of enzymes. Extremophiles 4: 91-98.
19. Manikandan M, Pašić L, Kannan V (2009) Optimization of growth media for obtaining high-cell density cultures of halophilic archaea (family Halobacteriaceae) by response surface methodology. Bioresour Technol 100: 3107-3112.
20. Margesin R, Schiner F (2001) Potential of halotolerant and halophilic microorganisms for biotechnology. Extremophiles 5: 73-83.
21. Maurer KH (2004) Detergent proteases. Curr Opin Biotechnol 15: 330-334.
22. McDonald CE, Chen LL (1965) The Lowry modification of the folin reagent for determination of proteinase activity. Anal Biochem 10: 175-177.
23. Nolasco H, Kushner DJ, Ochoa JL (2002) Purification and properties of an extracellular halophilic serine-protease from Haloferax mediterranei. J Mex Chem Soc 46: 202-211.
24. Pašić L, Herzog Velikonja B, Poklar Ulrih N (2008) Optimization of the culture conditions for the production of a bacteriocin from halophilic archaeon Sech7a. Prep Biochem Biotechnol 38: 229-245.
25. Rao MB, Tanksale AM, Ghatge MS, Deshpande VV (1998) Molecular and biotechnological aspects of microbial proteases. Microbiol Mol Biol Rev 62: 597-635.
26. Ruiz DM, De Castro RE (2007) Effect of organic solvents on the activity and stability of an extracellular protease secreted by the haloalkaliphilic archaeon Natrialba magadii. J Ind Microbiol Biotechnol 34: 111-115.
27. Ryu K, Kim J, Dordick JS (1994) Catalytic properties and potential of an extracellular protease from an extreme halophile. Enzyme Microb Tech 16: 266-275.
28. Serdakowski AL, Dordick JS (2008) Activating enzymes for use with organic solvents. In: Carrea G, Riva S (eds) Organic synthesis with enzymes in non-aqueous media. Wiley, New York, pp 47-75.
29. Shi WL, Zhong CQ, Tang B, Shen P (2007) Purification and characterization of extracellular halophilic protease from Natrinema sp. R6-5. Wei Sheng Wu Xue Bao 47: 161-163.
30. Stan-Lotter H, Doppler E, Jarosh M, Radax C, Gruber C, Inatomi KI (1999) Isolation of a chymotrypsinogen B-like enzyme from the archaeon Natronomonas pharaonis and other halobacteria. Extremophiles 3: 153-161.
31. Stepanov VM, Rudenskaya GN (1983) Proteinase affinity chromatography on bacitracin-Sepharose. J Appl Biochem 5: 420-428.
32. Stepanov VM, Rudenskaya GN, Revina LP, Gryaznova YB, Lysogorskaya EN, Filippova IY, Ivanova II (1992) A serine proteinase of archaebacterium, Halobacterium mediterranei, a homologue of an eubacterial subtilisin. Biochem J 285: 281-286.
33. Studdert CA, De Castro RE, Herrera Seitz MK, Sanchez JJ (1997) Detection and preliminary characterization of extracellular proteolytic activities of the haloalkaliphilic archaeon Natronococcusoccultus. Arch Microbiol 168: 532-535.
34. Studdert CA, Herrera Seitz MK, Plasencia Gil MI, Sanchez JJ, De Castro RE (2001) Purification, biochemical characterization of the haloalkaliphilic archeon Natronococcus occultus extracellular serine protease. J Basic Microbiol 41: 375-383.
35. Thongthai C, Gildberg A (2005) Asian fish sauce as a source of nutrition. In: Shahidi F, Ho C (eds) Asian functional foods. CRC press, USA, pp 215-267.
36. Vidyasagar M, Prakash S, Litchfield C, Sreeramulu K (2006) Purification and characterization of a thermostable, haloalkaliphilic extracellular serine protease from the extreme halophilic archaeon Halogeometricum borinquense strain TSS101. Archaea 2: 51-57.
37. Wolz RL (1999) Strategies for inhibiting proteases of unknown mechanism. In: Sterchi EE, Stöcker W (eds) Proteolytic enzymes tools and targets. Springer, New York, pp 90-108.