Isolation of a chymotrypsinogen B-like enzyme from the archaeon Natronomonas pharaonis and other halobacteria

Extremophiles

Volumn 3, Issue 2, 1999, Pages 153-161

  Stan Lotter, Helga ^a   Doppler, Edith ^a   Jarosch, Marina ^b   Radax, Christian ^a   Gruber, Claudia ^a   Inatomi, Ken Ichi ^c  

^a Inst of Genet and General Biology   (Austria)

^b Zentrum fur Ultrastrukturforschung   (Austria)

^c MITSUBISHI ELECTRIC CORPORATION   (Japan)

Author keywords

Archaea; Chymotrypsinogen; Evolution; Natronobacteria; Natronomonas pharaonis; Serine protease

Indexed keywords

CHYMOTRYPSINOGEN; ENZYME ACTIVITY; ENZYME INHIBITION; ENZYME ISOLATION; ENZYME KINETICS;

NON-PROGRAMMATIC;

AMINO ACID SEQUENCE; ANIMALS; CATTLE; CHYMOTRYPSINOGEN; ENDOPEPTIDASES; ENZYME PRECURSORS; HUMANS; MOLECULAR SEQUENCE DATA; NATRONOBACTERIUM; SEQUENCE HOMOLOGY, AMINO ACID;

ARCHAEA; BOVINAE; HALOBACTERIA; NATRONOBACTERIUM; NATRONOMONAS; NATRONOMONAS PHARAONIS; VERTEBRATA;

EID: 0033136918     PISSN: 14310651     EISSN: None     Source Type: Journal    
DOI: 10.1007/s007920050111     Document Type: Article

References (44)

1. Barrett AJ, Rawlings ND (1993) The many evolutionary lines of peptidases. In: Aviles FX (ed) Innovations in proteases and their inhibitors. Walter de Gruyter, Berlin, pp 13-30
2. Bult CJ, White O, Olsen GJ, Zhou L, Fleischmann RD, Sutton GG, Blake JA, FitzGerald LM, Clayton RA, Gocayne JD, Kerlavage AR, Dougherty BA, Tomb JF, Adams MD, Reich CI, Overbeek R, Kirkness EF, Weinstock KG, Merrick JM, Glodek A, Scott JL, Geoghagen NSM, Weidman JF, Fuhrmann JL, Nguyen D, Utterback TR, Kelley JM, Peterson JD, Sadow PW, Hanna MC, Cotton MD, Roberts KM, Hurst MA, Kaine BP, Borodovsky M, Klenk HP, Fraser CM, Smith HO, Woese CR, Venter JC (1996) Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii. Science 273:1017-1140
3. Crestfield AM, Moore S, Stein WH (1963) The preparation and enzymatic hydrolysis of reduced and S-carboxymethylated proteins. J Biol Chem 238:622-627
4. Eisenberg H, Wachtel EJ (1987) Structural studies of halophilic proteins, ribosomes, and organelles of bacteria adapted to extreme salt concentrations. Annu Rev Biophys Chem 16:69-92
5. Fricke D, Parchmann O, Aurich H (1993) Membrane-bound proteinases of Halobacterium halobium. J Basic Microbiol 33:9-18
6. Geiger R (1984) Pancreatic elastase. In: Bergmeyer HU (ed) Methods of enzymatic analysis, vol V. Verlag Chemie. Weinheim, pp 170-176
7. + ATPase: implications for the origin of cukaryotes. Proc Natl Acad Sci USA 86:6661-6665
8. Gruber C. Stan-Lotter H (1997) Western blot of stained proteins from dried polyacrylamide gels. Anal Biochem 253:125-127
9. Hess GP (1971) Chymotrypsin - chemical properties and catalysis. In: Boyer PD (ed) The enzymes, vol III, 3rd edn. Academic Press. New York, pp 213-248
10. Hochstein LI, Lang F (1991) Purification and properties of a dissimilatory nitrate reductase from Haloferax denitrificans. Arch Biochem Biophys 288:380-385
11. Hochstein LI, Kristjansson H, Altekar W (1987) The purification and subunit structure of a membrane-bound ATPase from the archaebacterium Halobacterium saccharovorum. Biochem Biophys Res Commun 147:295-300
12. Izotova LS, Strongin AY, Chekulaeva LN, Sterkin VE, Ostoslavskaya VI, Lyublinskaya LA, Timokhina EA, Stepanov VM (1983) Purification and properties of serine protease from Halobacterium halobium. J Bacteriol 155:826-830
13. Kamekura M, Seno Y, Holmes ML, Dyall-Smith ML (1992) Molecular cloning and sequencing of the gene for a halophilic alkaline serine protease (halolysin) from an unidentified halophilic archaea strain (171 P1) and expression of the gene in Haloferax volcanii. J Bacteriol 174:736-742
14. Kamekura M, Dyall-Smith ML (1995) Taxonomy of the family Halobacteriaceae and the description of two new genera Halorubrobacterium and Natrialba. J Gen Appl Microbiol 41:333-350
15. Kamekura M, Seno Y, Dyall-Smith ML (1996) Halolysin R4, a serine proteinase from the halophilic archaeon Haloferax mediterranei; gene cloning, expression and structural studies. Biochim Biophys Acta 1294:159-167
16. Kamekura M, Dyall-Smith ML, Upasani V, Ventosa A, Kates M (1997) Diversity of alkaliphilic halobacteria: proposal for transfer of Natronobacterium vacuolatum, Natronobacterium magadii and Natronobacteriun pharaonis to Halorubrum, Natrialba, and Natronomonas gen. nov., respectively, as Halorubrum vacuolatum comb. nov., Natrialba magadii comb. nov., and Natronomonas pharaonis, comb. nov., respectively. Int J Syst Bacteriol 47:853-857
17. Klenk HP, Clayton RA, Tomb J, White O, Nelson KE, Ketchum KA, Dodson RJ, Gwinn M, Hickey EK, Peterson JD, Richardson DL, Kerlavage AR, Graham DE, Kyrpides NC, Fleischmann RD, Quackenbush J, Lee NH, Sutton GG, Gill S, Kirkness EF, Dougherty BA, McKenney K, Adams MD, Loftus B, Peterson S, Reich CI, McNeil LK, Badger JH, Glodek A, Zhou L, Overbeek R, Gocayne JD, Weidman JF, McDonald L, Utterback T, Cotton MD, Spriggs T, Artiach P, Kaine BP, Sykes SM, Sadow PW, D'Andrea KP, Bowman C, Fujii C, Garland SA, Mason TM, Olsen GJ, Fraser CM, Smith HO, Woese CR, Venter JC (1997) The complete genome sequence of the hyperthermophilic, sulphate-reducing archaeon Archaeoglobus fulgidus. Nature (Lond) 390:364-370
18. Koonin EV, Mushegian AR, Galperin MY, Walker DR (1997) Comparison of archaeal and bacterial genomes: computer analysis of protein sequences predicts novel functions and suggests a chimeric origin for the Archaea. Mol Microbiol 25:619-637
19. Laemmli UK (1970) Cleavage of structural proteins during the assembly of the head of bacleriophage T4. Nature (Lond) 227:680-685
20. Lowry OH, Rosebrough NJ, Farr AL, Randall RJ (1951) Protein measurement with the Folin phenol reagent. J Biol Chem 193:265-275
21. Lundblad RL (1995) The modification of cystine - cleavage of disulfide bonds. In: Lundblad RL (ed) Techniques in protein modification. CRC Press, Boca Raton, pp 91-96
22. Margulis L (1996) Archaeal-eubacterial mergers in the origin of eukarya: phylogenetic classification of life. Proc Natl Acad Sci USA 93:1071-1076
23. Martin W, Müller M (1998) The hydrogen hypothesis for the first eukaryote. Nature (Lond) 392:37-41
24. May BP, Dennis PP (1987) Superoxide dismutase from the extremely halophilic archaebacterium Halobacterium cutirubrum. J Bacteriol 169:1417-1422
25. Nelson N (1992) Evolution of organellar proton-ATPases. Biochem Biophys Acta 1100:109-124
26. Nelson N (1995) Molecular and cellular biology of F- and V-ATPases. In: Nelson N (ed) Organellar proton ATPases. Springer New York, pp 1-27
27. Norberg P, von Hofsten B (1969) Proteolytic enzymes from extremely halophilic bacteria. J Gen Microbiol 55:251-256
28. Reistad R (1970) On the composition and nature of the bulk protein of extremely halophilic bacteria. Arch Microbiol 71:353-360
29. Schägger H, von Jagow G (1987) Tricine-sodium dodecyl sulfate polyacrylamide gel electrophoresis for the separation of proteins in the range from 1 to 100kDa. Anal Biochem 166:368-379
30. Scharf B, Wittenberg R, Engelhard M (1997) Electron transfer proteins from the haloalkaliphilic archaeon Natronobacterium pharaonis: Possible components of the respiratory chain include cytochrome bc and a terminal oxidase cytochrome ba3. Biochemistry 36:4471-4479
31. Schmitt W, Rdest U, Goebel W (1990) Efficient high-performance liquid chromatographic system for the purification of a halobacterial serine protease. J Chromatogr 521:211-220
32. Smillie LB, Furka A, Nagabhusan N, Stevenson KJ, Parkes CO (1968) Structure of chymotrypsinogen B compared with chymotrypsinogen A and trypsinogen. Nature (Lond) 218:343-346
33. Soliman GSH, Trüper HG (1982) Halobacterium pharaonis sp. nov., a new, extremely haloalkaliphilic archaebacterium with low magnesium requirement. Zentralbl Bakteriol Hyg Abt Orig C3:318-329
34. Soppa J, Duschl J, Oesterhelt D (1993) Bacterio-opsin, halo-opsin and sensory opsin I of the halobacterial isolate Halobacterium strain sp. SG1: three new members of a growing family. J Bacteriol 175:2720-2716
35. 1 ATPase of Escherichia coli and of the structural asymmetry of its βsubunits. Eur J Biochem 154:321-327
36. 1 moiety from the Escherichia coli ATP synthase. Eur J Biochem 179:155-160
37. Stepanov VM, Rudenskaya GN, Revina LP, Gryaznova YB, Lysogorskaya EL, Filippova IY, Ivanova II (1992) A senne protease of an archaebacterium, Halobacterium mediterranei. A homologue of eubacterial subtilisins. Biochem J 285:281-286
38. -)-ATPase. Annu Rev Cell Dev Biol 13:779-808
39. Studdert CA, De Castro RE, Seitz KH, Sanchez JJ (1997) Detection and preliminary characterization of extracellular proteolytic activities of the haloalkaliphilic archaeon Natronococcus occultus. Arch Microbiol 168:532-535
40. Tindall BJ, Ross HNM, Grant WD (1984) Natronobacterium gen. nov. and Natronococcus gen. nov., two new genera of haloalkaliphilic archaebacteria. Syst Appl Microbiol 5:41-57
41. Tomlinson GA, Hochstein LI (1976) Halobacterium saccharovorum sp.nov., a carbohydrate-metabolizing, extremely halophilic bacterium. Can J Microbiol 22:587-591
42. Towbin H, Staehlin T, Gordon J (1979) Electrophoretic transfer of proteins from polyacrylamide to nitrocellulose sheets: procedure and some applications. Proc Natl Acad Sci USA 76:4350-4354
43. Woese CR, Kandler O, Wheelis ML (1990) Towards a natural system of organisms: proposal for the domains Archaea, Bacteria and Eukarya. Proc Natl Acad Sci USA 87:4576-4579
44. Zillig W (1991) Comparative biochemistry of Archaea and Bacteria. Curr Opinion Genet Dev 1:544-551