Single-step procedure for the isolation of proteins at near-native conditions from mammalian tissue for proteomic analysis on antibody microarrays

Journal of Proteome Research

Volumn 9, Issue 2, 2010, Pages 963-971

  Alhamdani, Mohamed Saiel Saeed ^a   Schröder, Christoph ^a   Werner, Jens ^b   Giese, Nathalia ^b   Bauer, Andrea ^a   Hoheisel, Jörg D ^a  

^a GERMAN CANCER RESEARCH CENTER DKFZ   (Germany)

^b UNIVERSITY OF HEIDELBERG   (Germany)

Author keywords

Antibody microarray; Compartmentalized protein; Detergents; Protein extraction

Indexed keywords

AMIDOSULFOBETAINE; AMPHOLYTE; ANTIBODY; BETAINE DERIVATIVE; BUFFER; CELL PROTEIN; CHOLIC ACID; DETERGENT; ETHYLENE GLYCOL DERIVATIVE; FLUORESCENT DYE; GAMMA GLUTAMYLTRANSFERASE; GLUTATHIONE TRANSFERASE; LACTATE DEHYDROGENASE; STYRENE GLYCOL; UNCLASSIFIED DRUG;

ANIMAL TISSUE; ARTICLE; CANCER CELL CULTURE; CANCER TISSUE; CELL LABELING; CELL MEMBRANE; CONTROLLED STUDY; ENZYME ACTIVITY; HEART; HUMAN; HUMAN CELL; HUMAN TISSUE; IMMUNOASSAY; LIVER; LUNG; MALE; MAMMAL; MICROARRAY ANALYSIS; NONHUMAN; PANCREAS; PRIORITY JOURNAL; PROTEIN ANALYSIS; PROTEIN FUNCTION; PROTEIN ISOLATION; PROTEIN QUALITY; PROTEOMICS; RAT; WESTERN BLOTTING;

ANIMALS; ANTIBODIES; CELL LINE, TUMOR; ELECTROPHORESIS, POLYACRYLAMIDE GEL; FLUORESCENT DYES; HUMANS; PROTEINS; PROTEOMICS; RATS;

MAMMALIA; RATTUS;

EID: 76149134712     PISSN: 15353893     EISSN: None     Source Type: Journal    
DOI: 10.1021/pr900844q     Document Type: Article

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