Subcellular protein extraction from human pancreatic cancer tissues

BioTechniques

Volumn 46, Issue 4, 2009, Pages 297-304

  Börner, Anette ^a   Warnken, Uwe ^a   Schnölzer, Martina ^a   Von Hagen, Jörg ^b   Giese, Nathalia ^c   Bauer, Andrea ^a   Hoheisel, Jörg D ^a  

^a GERMAN CANCER RESEARCH CENTER DKFZ   (Germany)

^b MERCK KGAA   (Germany)

^c UNIVERSITY OF HEIDELBERG   (Germany)

Author keywords

Cancer; Disease; Pancreas; Protein extraction; Protein fractionation

Indexed keywords

6 PHOSPHOFRUCTOKINASE; ACTININ; ALANINE TRANSFER RNA LIGASE; ALPHA ACTININ 4; ATP DEPENDENT DNA HELICASE II; CALPAIN 1; CELLULAR APOPTOSIS SUSCEPTIBILITY PROTEIN; CLATHRIN HEAVY CHAIN 1; COATOMER PROTEIN; COLLAGENASE; ELONGATION FACTOR; EUKARYOTIC TRANSLATION ELONGATION FACTOR 2; GELSOLIN; GLYCINE TRANSFER RNA LIGASE; GLYCOGEN PHOSPHORYLASE; HEAT SHOCK PROTEIN; HELICASE; KARYOPHERIN; LIVER ENZYME; NUCLEAR PROTEIN; PROTEIN; PROTEIN KHSRP; STAT3 PROTEIN; THREONINE TRANSFER RNA LIGASE; TUMOR REJECTION ANTIGEN; UBIQUITIN PROTEIN LIGASE; UNCLASSIFIED DRUG; UNINDEXED DRUG; VACUOLAR SORTING PROTEIN 35; VALOSIN CONTAINING PROTEIN; VINCULIN; TUMOR PROTEIN;

ANIMAL TISSUE; ARTICLE; CANCER TISSUE; CELL ISOLATION; CELL ORGANELLE; CONTROLLED STUDY; CYTOSOLIC FRACTION; EFFECTOR CELL; EXTRACELLULAR MATRIX; FRACTIONATION; HISTOCHEMISTRY; HUMAN; HUMAN CELL; HUMAN TISSUE; MASS SPECTROMETRY; MEMBRANE; NONHUMAN; PANCREAS CANCER; PROTEIN CONFORMATION; PROTEIN CONTENT; PROTEIN DETERMINATION; PROTEIN INTERACTION; PROTEIN ISOLATION; PROTEIN QUALITY; PROTEIN STABILITY; PROTEIN STRUCTURE; RAT; TISSUE PREPARATION; TISSUE SLICE; TUMOR; ANIMAL; CELL FRACTIONATION; CHEMISTRY; ELECTROSPRAY MASS SPECTROMETRY; HISTOLOGY; ISOLATION AND PURIFICATION; METABOLISM; METHODOLOGY; PANCREAS TUMOR; PATHOLOGY; POLYACRYLAMIDE GEL ELECTROPHORESIS; REPRODUCIBILITY; SWINE; TUMOR CELL LINE; TWO DIMENSIONAL GEL ELECTROPHORESIS;

ANIMALS; CELL FRACTIONATION; CELL LINE, TUMOR; COLLAGENASES; ELECTROPHORESIS, GEL, TWO-DIMENSIONAL; ELECTROPHORESIS, POLYACRYLAMIDE GEL; HISTOCYTOLOGICAL PREPARATION TECHNIQUES; HUMANS; NEOPLASM PROTEINS; PANCREATIC NEOPLASMS; RATS; REPRODUCIBILITY OF RESULTS; SPECTROMETRY, MASS, ELECTROSPRAY IONIZATION; SUBCELLULAR FRACTIONS; SWINE;

EID: 67650504959     PISSN: 07366205     EISSN: None     Source Type: Journal    
DOI: 10.2144/000113090     Document Type: Article

References (29)

1. Harry, J.L., M.R. Wilkins, B.R. Herbert, N.H. Packer, A.A. Gooley, and K.L. Williams. 2000. Proteomics: capacity versus utility. Electrophoresis 21:1071-1081.
2. Miklos, G.L. and R. Maleszka. 2001. Protein functions and biological contexts. Proteomics 1:169-178.
3. Hogeboom, G.H., W.C. Schneider, and G.E. Palade. 1948. Purification of diphosphopyridine nucleotide by counter-current distribution. J. Biol. Chem. 172:619-635.
4. Fleischer, B., S. Fleischer, and H. Ozawa. 1969. Isolation and characterization of golgi membranes from bovine liver. J. Cell Biol. 43:59-79.
5. Shaw, M.M. and B.M. Riederer. 2003. Sample preparation for two-dimensional gel electrophoresis. Proteomics 3:1408-1417. (Pubitemid 37048065)
6. Ramsby, M.L. and G.S. Makowski. 1999. Differential detergent fractionation of eukaryotic cells. Analysis by two-dimensional gel electrophoresis. Methods Mol. Biol. 112:53-66.
7. Olsavsky, K.M., J.L. Page, M.C. Johnson, H. Zarbl, S.C. Strom, and C.J. Omiecinski. 2007. Gene expression profiling and differentiation assessment in primary human hepatocyte cultures, established hepatoma cell lines, and human liver tissues. Toxicol. Appl. Pharmacol. 222:42-56.
8. Yunis, A.A., G.K. Arimura, and D.J. Russin. 1977. Human pancreatic carcinoma (MIA PaCa-2) in continuous culture: sensitivity to asparaginase. Int. J. Cancer 19:128-135.
9. Ramana, K.V., D. Chandra, S. Srivastava, A. Bhatnagar, B.B. Aggarwal, and S.K. Srivastava. 2002. Aldose reductase mediates mitogenic signaling in vascular smooth muscle cells. J. Biol. Chem. 277:32063-32070.
10. Sinha, P., J. Poland, M. Schnolzer, and T. Rabilloud. 2001. A new silver staining apparatus and procedure for matrix-assisted laser desorption/ ionization-time of flight analysis of proteins after two-dimensional electrophoresis. Proteomics 1:835-840.
11. Klimmeck, D., U. Mayer, N. Ungerer, U. Warnken, M. Schnölzer, S. Frings, and F. Möhrlen. 2008. Calcium-signaling networks in olfactory receptor neurons. Neuroscience 151:901-912.
12. Stahl, M., M. Retzlaff, M. Nassal, and J. Beck. 2007. Chaperone activation of the hepadnaviral reverse transcriptase for template RNA binding is established by the Hsp70 and stimulated by the Hsp90 system. Nucleic Acids Res. 35:6124-6136. (Pubitemid 350018575)
13. Leonard, W.J. 2001. Role of Jak kinases and STATs in cytokine signal transduction. Int. J. Hematol. 73:271-277.
14. David, V., F. Hochstenbach, S. Rajagopalan, and M.B. Brenner. 1993. Interaction with newly synthesized and retained proteins in the endoplasmic reticulum suggests a chaperone function for human integral membrane protein IP90 (calnexin). J. Biol. Chem. 268:9585-9592. (Pubitemid 23146016)
15. Guller, M., K. Toualbi-Abed, A. Legrand, L. Michel, A. Mauviel, D. Bernuau, and F. Daniel. 2008. C-Fos overexpression increases the proliferation of human hepatocytes by stabilizing nuclear Cyclin D1. World J. Gastroenterol. 14:6339-6346.
16. Foisner, R. 2003. Cell cycle dynamics of the nuclear envelope. ScientificWorldJournal 3:1-20.
17. Pittenger, J.T., J.F. Hess, M.S. Budamagunta, J.C. Voss, and P.G. Fitzgerald. 2008. Identification of phosphorylation-induced changes in vimentin intermediate filaments by site-directed spin labeling and electron paramagnetic resonance. Biochemistry 47:10863-10870.
18. 2 bath for the powdering of tissue with a mortar and pestle. Anal. Biochem. 269:213-214.
19. Potter, V.R. and C.A. Elvehjem. 1936. A modified method for the study of tissue oxidations. J. Biol. Chem. 114:495-504.
20. Potter, V.R. 1946. The assay of animal tissues for respiratory enzymes IV: cell structure in relation to fatty acid oxidation. J. Biol. Chem. 163:437-446.
21. Brandhorst, H., D. Brandhorst, M.D. Brendel, B.J. Hering, and R.G. Bretzel. 1998. Assessment of intracellular insulin content during all steps of human islet isolation procedure. Cell Transplant. 7:489-495. (Pubitemid 29046553)
22. Swanson, C.J., B.J. Olack, D. Goodnight, L. Zhang, and T. Mohanakumar. 2001. Improved methods for the isolation and purification of porcine islets. Hum. Immunol. 62:739-749.
23. Bohman, S., A. Andersson, and A. King. 2006. No differences in efficacy between noncultured and cultured islets in reducing hyperglycemia in a nonvascularized islet graft model. Diabetes Technol. Ther. 8:536-545.
24. Mollenhauer, J., I. Roether, and H.F. Kern. 1987. Distribution of extracellular matrix proteins in pancreatic ductal adenocarcinoma and its influence on tumor cell proliferation in vitro. Pancreas 2:14-24.
25. Imamura, T., H. Iguchi, T. Manabe, G. Ohshio, T. Yoshimura, Z.H. Wang, H. Suwa, S. Ishigami, et al. 1995. Quantitative analysis of collagen and collagen subtypes I, III, and V in human pancreatic cancer, tumor-associated chronic pancreatitis, and alcoholic chronic pancreatitis. Pancreas 11:357-364.
26. Newton, S.S., A. Dow, R. Terwilliger, and R. Duman. 2002. A simplified method for combined immunohistochemistry and in-situ hybridization in fresh-frozen, cryocut mouse brain sections. Brain Res. Brain Res. Protoc. 9:214-219.
27. Boltze, C., R. Schneider-Stock, G. Aust, C. Mawrin, H. Dralle, A. Roessner, and C. Hoang-Vu. 2002. CD97, CD95 and Fas-L clearly discriminate between chronic pancreatitis and pancreatic ductal adenocarcinoma in perioperative evaluation of cryocut sections. Pathol. Int. 52:83-88.
28. Cooper, G.M. 2000. The Cell: A Molecular Approach, 2nd ed. Sinauer Associates Inc., Sunderland, MA.
29. Kwon, M., T.J. MacLeod, Y. Zhang, and D.M. Waisman. 2005. S100A10, annexin A2 and annexin a2 heterotetramer as candidate plasminogen receptors. Front. Biosci. 10:300-325.