Low-density macroarray targeting non-locus of enterocyte effacement effectors (nle genes) and major virulence factors of Shiga toxin-producing Escherichia coli (STEC): A new approach for molecular risk assessment of STEC isolates

Applied and Environmental Microbiology

Volumn 76, Issue 1, 2010, Pages 203-211

  Bugarel, Marie ^a   Beutin, Lothar ^b   Fach, Patrick ^a  

^a Healthy Pigs Production and Experimentation Section   (France)

^b FEDERAL INSTITUTE FOR RISK ASSESSMENT   (Germany)

Author keywords

[No Author keywords available]

Indexed keywords

CLINICAL ISOLATES; E. COLI; GENOMIC ISLANDS; GENOTYPING; MACROARRAYS; NEW APPROACHES; PUBLIC HEALTH; SEROTYPES; SHIGA TOXIN; SPECIFIC DETECTION; VIRULENCE FACTORS;

ANIMALS; ESCHERICHIA COLI; GENE TRANSFER; GENES; HEALTH RISKS; RISK ASSESSMENT; RISK MANAGEMENT;

STRAIN;

ESCHERICHIA COLI PROTEIN; VIRULENCE FACTOR;

GENE; GENOME; MOLECULAR ANALYSIS; PATHOTYPE; PUBLIC HEALTH; TOXIN; VIRULENCE;

ANIMAL; ANIMAL DISEASE; ARTICLE; BACTERIAL GENE; CHILD; COMPARATIVE STUDY; DNA MICROARRAY; ESCHERICHIA COLI INFECTION; FOOD CONTROL; GENETICS; GENOMIC ISLAND; GENOTYPE; HUMAN; INFANT; ISOLATION AND PURIFICATION; METHODOLOGY; MICROBIOLOGY; PRESCHOOL CHILD; RISK ASSESSMENT; SHIGA TOXIN PRODUCING ESCHERICHIA COLI;

ANIMALS; CHILD; CHILD, PRESCHOOL; ENVIRONMENTAL MICROBIOLOGY; ESCHERICHIA COLI INFECTIONS; ESCHERICHIA COLI PROTEINS; FOOD MICROBIOLOGY; GENES, BACTERIAL; GENOMIC ISLANDS; GENOTYPE; HUMANS; INFANT; OLIGONUCLEOTIDE ARRAY SEQUENCE ANALYSIS; RISK ASSESSMENT; SHIGA-TOXIGENIC ESCHERICHIA COLI; VIRULENCE FACTORS;

ANIMALIA; ESCHERICHIA COLI;

EID: 76149130722     PISSN: 00992240     EISSN: 10985336     Source Type: Journal    
DOI: 10.1128/AEM.01921-09     Document Type: Article

References (37)

1. Anonymous. 2005. European Commission Annual Report 2005: surveillance of enteric pathogens in Europe and beyond; 1786/2002/EC, International surveillance network for the enteric infections-Salmonella, VTEC O157 and Campylobacter. European Commission, Brussels, Belgium.
2. Beutin, L., A. Miko, G. Krause, K. Pries, S. Haby, K. Steege, and N. Albrecht. 2007. Identification, of human-pathogenic strains of Shiga toxin-producing Escherichia coli from food by a combination of serotyping and molecular typing of Shiga toxin genes. Appl. Environ. Microbiol. 73:4769-4775.
3. Beutin, L., G. Krause, S. Zimmermann, S. Kaulfuss, and K. Gleier. 2004. Characterization of Shiga toxin-producing Escherichia coli strains isolated from human patients in Germany over a 3-year period. J. Clin. Microbiol. 42:1099-1108.
4. Beutin, L., H. Steinruck, G. Krause, K. Steege, S. Haby, G. Hullsch, and B. Appel. 2007. Comparative evaluation of the Ridascreen®Verotoxin enzyme immunoassay for detection of Shiga-toxin producing strains of Escherichia coli (STEC) from food and other sources. J. Appl. Microbiol. 102:630-639.
5. Beutin, L., S. Jahn, and P. Fach. 2009. Evaluation of the 'GeneDisc' realtime PCR system, for detection of enterohaemorrhagic Escherichia coli (EHEC) O26, O103, O111, O145 and O157 strains according to their virulence markers and their O- and H-antigen-associated genes. J. Appl. Microbiol. 106:1122-1132.
6. Beutin, L. 2006. Emerging enterohaemorrhagic Escherichia coli, causes and effects of the rise of a human pathogen. J. Vet. Med. B Infect. Dis. Vet. Public Health 53:299-305.
7. Bielaszewska, M., R. Kock, A. W. Friedrich, C. Von Eiff, L. B. Zimmerhackl, H. Karch, and A. Mellmann. 2007. Shiga toxin-mediated hemolytic uremic syndrome: time to change the diagnostic paradigm? PLoS One 2:e1024.
8. Brooks, J. T., E. G. Sowers, J. G. Wells, K. D. Greene, P. M. Griffin, R. M. Hoekstra, and N. A. Strockbine. 2005. Non-O157 Shiga toxin-producing Escherichia coli infections in the United States, 1983-2002. J. Infect. Dis. 192:1422-1429.
9. Coombes, B. K., M. E. Wickham, M. Mascarenhas, S. Gruenheid, B. B. Finlay, and M. A. Karmali. 2008. Molecular analysis as an aid to assess the public health risk of non-O157 Shiga toxin-producing Escherichia coli strains. Appl. Environ. Microbiol. 74:2153-2160.
10. Creuzburg, K., and H. Schmidt. 2007. Molecular characterization, and distribution of genes encoding members of the type III effector nleA family among pathogenic Escherichia coli strains. J. Clin. Microbiol. 45:2498-2507. (Pubitemid 47295480)
11. EFSA. 2007. Scientific opinion of the panel on biological hazards on a request from EFSA on monitoring of verotoxigenic Escherichia coli (VTEC) and identification of human pathogenic types. EFSA J. 579:1-61.
12. Eklund, M., F. Scheutz, and A. Siitonen. 2001. Clinical isolates of non-O157 Shiga toxin-producing Escherichia coli serotypes, virulence characteristics, and molecular profiles of strains of the same serotype. J. Clin. Microbiol. 39:2829-2834.
13. Elliott, S. J., L. A. Wainwright, T. K. McDaniel, K. G. Jarvis, Y. K. Deng, L. C. Lai, B. P. McNamara, M. S. Donnenberg, and J. B. Kaper. 1998. The complete sequence of the locus of enterocyte effacement (LEE) from enteropathogenic Escherichia coli E2348/69. Mol. Microbiol. 28:1-4.
14. Ewing, W. H. 1986. Differentiation of Enterobacteriaceae by biochemical reactions, p. 47-72. In W. H. Ewing (ed.), Edwards and Swing's identification of Enterobacteriaceae. Elsevier Science .Publishing Co., New York, NY.
15. Fach, P., S. Perelle, F. Dilasser, and J. Grout. 2001. Comparison between a PCRELISA test and the Vero cell assay for detecting Shiga toxin-producing Escherichia coli in daily products and characterization of virulence traits of the isolated strains. J. Appl. Microbiol. 90:809-818.
16. Friedrich, A. W., W. Zhang, M. Bielaszewska, A. Mellmann, R. Köck, A. Fruth, H. Tschäpe, and H. Karch. 2007. Prevalence, virulence profiles, and clinical significance of Shiga toxin-negative variants of enterohemorrhagic Escherichia coli O157 infection in humans. Clin. Infect. Dis. 45:39-45.
17. Karmali, M. A., B. T. Steele, M. Petric, and C. Lim. 1983. Sporadic cases of haemolytic-uraemic syndrome associated with faecal cytotoxin and cytotoxinproducing Escherichia coli in stools. Lancet 1:619-620.
18. Karmali, M. A., M. Mascarenhas, S. Shen, K. Ziebell, S. Johnson, R. Reid-Smith, J. Isaac-Renton, C. Clark, K. Rahn, and J. B. Kaper. 2003. Association of genomic O island 122 of Escherichia coli EDL 933 with verocytotoxin-producing Escherichia coli seropathotypes that are linked to epidemic and/or serious disease. J. Clin. Microbiol. 41:4930-4940.
19. Kim, J., J. Nietfeldt, and A. K. Benson. 1999. Octamer-based genome scanning distinguishes a unique subpopulation of Escherichia coli O157:H7 strains in cattle. Proc. Natl. Acad. Sci. USA 96:13288-13293.
20. Konczy, P., K. Ziebell, M. Mascarenhas, A. Choi, C. Michaud, A. M. Kropinski, T. S. Whittam, M. Wickham, B. Finlay, and M. A. Karmali. 2008. Genomic O island 122, locus for enterocyte effacement, and the evolution of virulent verocytotoxin-producing Escherichia coli. J. Bacteriol. 190:5832-5840.
21. Kozub-Witkowski, E., G. Krause, G. Frankel, D. Kramer, B. Appel, and L. Beutin. 2008. Serotypes and virutypes of enteropathogenic and enterohaemorrhagic Escherichia coli strains from stool samples of children with diarrhoea in Germany. J. Appl. Microbiol. 104:403-410.
22. Levine, M. M. 1987. Escherichia coli that cause diarrhea.- enterotoxigenic, enteropathogenic, enteroinvasive, enterohemorrhagic, and enteroadherent. J. Infect. Dis. 155:377-389.
23. Maidhof, H., B. Guerra, S. Abbas, H. M. Elsheikha, T. S. Whittam, and L. Beutin. 2002. A multiresistant clone of Shiga toxin-producing Escherichia coli O118:[H16] is spread in cattle and humans over different European countries. Appl. Environ. Microbiol. 68:5834-5842.
24. McLean, C., K. A. Bettelheim, A. Kuzevski, L. Falconer, and S. P. Djordjevic. 2005. Isolation of Escherichia coli O5:11-, possessing genes for Shiga toxin. 1, intimin- β and enterohaemolysin, from an intestinal biopsy from an adult case of bloody diarrhoea: evidence for two distinct O5:H-pathotypes. J. Med. Microbiol. 54:605-607.
25. Nataro, J. P., and J. B. Kaper. 1998. Diarrheagenic Escherichia coli. Clin. Microbiol. Rev. 11:142-201.
26. Nielsen, E. M., and M. T. Andersen. 2003. Detection, and characterization of verocytotoxin-producing Escherichia coli by automated 5 nuclease PCR assay. J. Clin. Microbiol. 41:2884-2893.
27. Oswald, E., H. Schmidt, S. Morabito, H. Karch, O. Marches, and A. Caprioli. 2000. Typing of intimin genes in human and animal enterohemorrhagic and enteropathogenic Escherichia coli characterization of a new intimin variant. Infect. Immun. 68:64-71.
28. Perelle, S., F. Dilasser, J. Grout, and P. Fach. 2004. Detection by 5-nuclease PCR of Shiga toxin producing Escherichia, coli O26, O55, O91, O103, O111, O113, O145 and O157:H7, associated with, the world's most frequent clinical cases. Mol. Cell. Probes 18:185-192.
29. Perna, N. T., G. F. Mayhew, G. Pósfai, S. Elliott, M. S. Donnenberg, J. B. Kaper, and F R. Blattner. 1998. Molecular evolution of a pathogenicity island from enterohemorrhagic Escherichia coli O157:H7. Infect. Immun. 66:3810-3817.
30. Riley, L W., R. S. Remis, S. D. Helgerson, H. B. McGee, J. G. Wells, B. R. Davis, R. J. Hebert, E. S. Olcott, L M. Johnson, N. T. Hargrett, P. A. Blake, and M. L. Cohen. 1983. Hemorrhagic colitis associated with a rare Escherichia coli serotype. N. Engl. J. Med. 308:681-685.
31. Schimmer, B., K. Nygard, H. M. Eriksen, J. Lassen, B. A. Lindstedt, L. T. Brandal, G. Kapperud, and P. Aavitsland. 2008. Outbreak of haemolytic uraemic syndrome in Norway caused by stx2-positive Escherichia coli O103: H25 traced to cured mutton sausages. BMC Infect. Dis. 8:41.
32. Tarr, C. L., and T. S. Whittam. 2002. Molecular evolution of the intimin gene in. O111. clones of pathogenic Escherichia coli. J. Bacteriol. 184:479-487.
33. Werber, D., L. Beutin, R. Piclmer, K. Stark, and A. Fruth. 2008. Shiga toxin-producing Escherichia coli serogroups in food and patients, Germany. Emerg. Infect. Dis. 14:1803-1806.
34. Wick, L. M., W. Qi, D. W. Lacher, and T. S. Whittam. 2005. Evolution of genomic content in the stepwise emergence of Escherichia coli O157:H7. J. Bacteriol. 187:1783-1791.
35. Wickham, M. E., C. Lupp, M. Mascarenhas, A. Vazquez, B. K. Coombes, N. F. Brown, B. A. Coburn, W. Deng, J. L. Puente, M. A. Karmali, and B. B. Finlay. 2006. Bacterial genetic determinants of non-O157 STEC outbreaks and hemolytic-uremic syndrome after infection. J. Infect. Dis. 194:819-827.
36. Yaradou, D. F., S. Hallier-Soulier, S. Moreau, F. Poty, Y. Hillion, M. Reyrolle, J. Andre, G. Festoc, K. Delabre, F. Vandenesch, J. Etienne, and S. Jarraud. 2007. Integrated real-time PCR for detection and monitoring of Legionella pneumophila in water systems. Appl. Environ. Microbiol. 73:1452-1456.
37. Zhang, W. L., B. Kohler, E. Oswald, L. Beutin, H. Karch, S. Morabito, A. Caprioli, S. Suerbaum, and H. Schmidt. 2002. Genetic diversity of intimin genes of attaching and effacing Escherichia coli strains. J. Clin. Microbiol. 40:4486-4492.