Results, conclusions, and recommendations of two ring trials for the detection and isolation of shiga (Vero) toxin producing Escherichia coli (STEC) from minced beef samples;Ergebnisse, Schlussfolgerungen und Empfehlungen aus zwei Ringversuchen zum Nachweis und zur Isolierung von Shiga (Vero) Toxin bildenden Escherichia coli (STEC) aus Hackfleischproben

Journal fur Verbraucherschutz und Lebensmittelsicherheit

Volumn 5, Issue 1, 2010, Pages 21-34

  Beutin, Lothar ^a   Martin, Annett ^b   Krause, Gladys ^a   Steege, Katja ^a   Haby, Sabine ^a   Pries, Karin ^a   Albrecht, Nadine ^a   Miko, Angelika ^a   Jahn, Silke ^a,c  

^a Mikrobielle Toxine Nationales Referenzlabor für Escherichia coli   (Germany)

^b Biometrie und mathematische Modellierung   (Germany)

^c Fachgruppe Antibiotikaresistenz und Resistenzdeterminanten   (Germany)

Author keywords

E. coli; Enzym Immunoassay; Hackfleisch; PCR; Ringversuch; Shigatoxin

Indexed keywords

ESCHERICHIA COLI;

EID: 75849154755     PISSN: 16615751     EISSN: None     Source Type: Journal    
DOI: 10.1007/s00003-009-0526-x     Document Type: Article

References (31)

1. Amtliche Sammlung von Untersuchungsverfahren nach § 35 LMBG (L 07. 18 1) (2002) Untersuchung von Lebensmitteln: Nachweis, Isolierung und Charakterisierung Verotoxin-bildender Escherichia coli (VTEC) in Hackfleisch mittels PCR und DNA-Hybridisierungstechnik. Beuth Verlag, Berlin.
2. Amtliche Sammlung von Untersuchungsverfahren nach § 64 LFGB (L 00. 00 92) (2006) Untersuchung von Lebensmitteln: Nachweis von Verotoxin-bildenden Escherichia coli Stämmen (VTEC) in Lebensmitteln tierischer Herkunft. Beuth Verlag, Berlin, pp 1-18.
3. Bettelheim KA, Beutin L (2003) Rapid laboratory identification and characterization of verocytotoxigenic (Shiga toxin producing) Escherichia coli (VTEC/STEC). J Appl Microbiol 95: 205-217.
4. Beutin L (2006) Emerging enterohaemorrhagic Escherichia coli, causes and effects of the rise of a human pathogen. J Vet Med B Infect Dis Vet Public Health 53: 299-305.
5. Beutin L, Steinruck H, Krause G, Steege K, Haby S, Hultsch G, Appel B (2007) Comparative evaluation of the Ridascreen((R)) Verotoxin enzyme immunoassay for detection of Shiga-toxin producing strains of Escherichia coli (STEC) from food and other sources. J Appl Microbiol 102: 630-639.
6. Busch U, Huber I, Messelhäuser U, Hörmansdorfer S, Sing A (2007) Nachweis Shigatoxin-bildender/Enterohämorrhagischer Escherichia coli (STEC/EHEC) mittels Real-Time PCR. J Verbr Lebensm 2: 144-148.
7. Caprioli A, Morabito S, Brugère H, Oswald E (2005) Enterohaemorrhagic Escherichia coli: emerging issues on virulence and modes of transmission. Vet Res 36: 289-311.
8. Collett D (1999) Modelling binary data. Chapman & Hall/CRC, Boca Raton.
9. Durso LM, Keen JE (2007) Shiga-toxigenic Escherichia coli O157 and non-Shiga-toxigenic E. coli O157 respond differently to culture and isolation from naturally contaminated bovine faeces. J Appl Microbiol 103: 2457-2464.
10. EFSA (2007) Scientific opinion of the panel on biological hazards on a request from EFSA on monitoring of verotoxigenic Escherichia coli (VTEC) and identification of human pathogenic types. EFSA J 579: 1-61.
11. Ellerbroek L, Alter T, Johne R, Nockler K, Beutin L, Helmuth R (2009) Tasks and duties of veterinary reference laboratories for food borne zoonoses. Bundesgesundheitsblatt 52: 208-213.
12. Gyles CL (2007) Shiga toxin-producing Escherichia coli: an overview. J Anim Sci 85: E45-E62.
13. Hussein HS, Bollinger LM (2008) Influence of selective media on successful detection of Shiga toxin-producing Escherichia coli in food, fecal, and environmental samples. Foodborne Pathog Dis 5: 227-244.
14. Islam MA, Heuvelink AE, Talukder KA, Zwietering MH, de Boer E (2006) Evaluation of immunomagnetic separation and PCR for the detection of Escherichia coli O157 in animal feces and meats. J Food Prot 69: 2865-2869.
15. ISO 16140 (2003) Microbiology of food and animal feeding stuffs - protocol for the validation of alternative methods. Geneva, Switzerland, pp 1-74.
16. ISO 16654 (2001) Microbiology of food and animal feeding stuffs - horizontal method for the detection of Escherichia coli O157. Geneva, Switzerland, pp 1-13.
17. Jahn S, Beutin L (2009) Evaluation of the "GeneDisc" Real-Time PCR system for detection of major EHEC type strains From different food matrices (minced meat, "Mettwurst", salami and raw milk). VTEC 2009, Program and Abstract Book. Asociación Argentina de Microbiología, Buenos Aires, pp 104-105.
18. Jahn S, Weber H, Beutin L (2008) Vergleich von Enzym Immunoassay und quantitativer Real Time PCR zum Nachweis von Shigatoxin produzierenden Escherichia coli (STEC) in Hackfleisch. J Verbr Lebensm 3: 385-395.
19. Johnson KE, Thorpe CM, Sears CL (2006) The emerging clinical importance of non-O157 Shiga toxin-producing Escherichia coli. Clin Infect Dis 43: 1587-1595.
20. Karch H, Tarr PI, Bielaszewska M (2005) Enterohaemorrhagic Escherichia coli in human medicine. Int J Med Microbiol 295: 405-418.
21. Langton SD, Chevennement R, Nagelkerke N, Lombard B (2002) Analysing collaborative trials for qualitative microbiological methods: accordance and concordance. Int J Food Microbiol 79: 175-181.
22. O'Brien SB, Duffy G, Daly D, Sheridan JJ, Blair IS, McDowell DA (2005) Detection and recovery rates achieved using direct plate and enrichment/immunomagnetic separation methods for Escherichia coli O157: H7 in minced beef and on bovine hide. Lett Appl Microbiol 41: 88-93.
23. Paton JC, Paton AW (2003) Methods for detection of STEC in humans. An overview. Methods Mol Med 73: 9-26.
24. Pulz M, Matussek A, Monazahian M, Tittel A, Nikolic E, Hartmann M, Bellin T, Buer J, Gunzer F (2003) Comparison of a shiga toxin enzyme-linked immunosorbent assay and two types of PCR for detection of shiga toxin-producing Escherichia coli in human stool specimens. J Clin Microbiol 41: 4671-4675.
25. Robert-Koch Institut (2006) Ausgewählte Zoonosen im Jahr 2005: Durch Lebensmittel übertragbare bakterielle gastrointestinale Infektionen. Epidemiol Bull 41: 351-356.
26. Robert-Koch Institut (2008) Erkrankungen durch Enterohämorrhagische Escherichia coli (EHEC). Epidemiol Bull 2: 11-21.
27. Sachs L (2002) Angewandte Statistik. Springer, Berlin, 10. Aufl., pp 472-473.
28. Scheutz F, Strockbine NA (2005) Genus I. Escherichia. In: Garrity GM, Brenner DJ, Krieg NR, Staley JT (eds) Bergey's manual of systematic bacteriology. Springer, Berlin, pp 607-624.
29. Tuttle J, Gomez T, Doyle MP, Wells JG, Zhao T, Tauxe RV, Griffin PM (1999) Lessons from a large outbreak of Escherichia coli O157: H7 infections: insights into the infectious dose and method of widespread contamination of hamburger patties. Epidemiol Infect 122: 185-192.
30. Werber D, Fruth A, Heissenhuber A, Wildner M, Prager R, Tschape H, Ammon A (2004) Shiga toxin-producing Escherichia coli O157 more frequently cause bloody diarrhoe than do non-O157 strains. J Infect Dis 189: 1335-1336.
31. Werber D, Beutin L, Pichner R, Stark K, Fruth A (2008) Shiga toxin-producing Escherichia coli serogroups in food and patients, Germany. Emerg Infect Dis 14: 1803-1806.