Expression and purification of ZEBRA fusion proteins and applications for the delivery of macromolecules into mammalian cells

Current Protocols in Protein Science

Volumn , Issue SUPPL.54, 2008, Pages

  Rothe, Romy ^a   Lenormand, Jean Luc ^a  

^a UNIV GRENOBLE ALPES   (France)

Author keywords

Endocytosis; Fusion protein; Intracellular distribution; Protein delivery; Protein expression; Therapeutic proteins

Indexed keywords

ANTISENSE OLIGONUCLEOTIDE; DNA; HYBRID PROTEIN; IMMEDIATE EARLY PROTEIN BZLF1; RECOMBINANT PROTEIN;

AFFINITY CHROMATOGRAPHY; BACTERIAL CELL; BATCH PROCESS; ESCHERICHIA COLI; EXPRESSION VECTOR; FLUORESCENCE MICROSCOPY; GEL MOBILITY SHIFT ASSAY; GENE CONSTRUCT; GENE EXPRESSION SYSTEM; HUMAN; HUMAN CELL; IMMUNOCYTOCHEMISTRY; LARGE SCALE PRODUCTION; MACROMOLECULE; MAMMAL CELL; METHODOLOGY; MOLECULAR PROBE; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; PROTEIN BINDING; PROTEIN DOMAIN; PROTEIN EXPRESSION; PROTEIN LOCALIZATION; PROTEIN PURIFICATION; RECOMBINANT DNA TECHNOLOGY; REVIEW; SUPERNATANT; ARTICLE; BINDING SITE; BIOTINYLATION; CELLULAR DISTRIBUTION; CLINICAL PROTOCOL; FLOW CYTOMETRY; FLUORESCENCE ACTIVATED CELL SORTING; HYDROPHOBICITY; IMMUNOBLOTTING; IMMUNOFLUORESCENCE; INTERNALIZATION; NONHUMAN; PROTEIN ANALYSIS; PROTEIN CLEAVAGE; PROTEIN DEGRADATION; PROTEIN DENATURATION; PROTEIN SYNTHESIS; RESTRICTION SITE; SEQUENCE ALIGNMENT; SIGNAL TRANSDUCTION;

ANIMALIA; BACTERIA (MICROORGANISMS); HUMAN HERPESVIRUS 4; MAMMALIA;

EID: 75749155041     PISSN: 19343655     EISSN: 19343663     Source Type: Journal    
DOI: 10.1002/0471140864.ps1811s54     Document Type: Review

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