A simple metaphase chromosome preparation from meristematic root tip cells of wheat for karyotyping or in situ hybridization

African Journal of Biotechnology

Volumn 9, Issue 3, 2010, Pages 314-318

  Mirzaghaderi, Ghader ^a  

^a UNIVERSITY OF KURDISTAN   (Iran)

Author keywords

Fluorescence in situ hybridization (FISH); Karyotyping; Squashing method; Wheat

Indexed keywords

COLCHICINE; HYDROCHLORIC ACID; ICE; NAPHTHALENE DERIVATIVE; WATER;

ARTICLE; C BANDING; CELL CYCLE ARREST; CHROMOSOME PREPARATION; CULTIVAR; GENOMIC IN SITU HYBRIDIZATION; KARYOTYPING; MERISTEM; METAPHASE CHROMOSOME; MONOLAYER CULTURE; NONHUMAN; PLANT ROOT; WHEAT;

TRITICUM AESTIVUM;

EID: 75749136076     PISSN: None     EISSN: 16845315     Source Type: Journal    
DOI: None     Document Type: Article

References (15)

1. Anamthawat-Jonsson K (2003). Preparation of chromosomes from plant leaf meristems for karyotype analysis and in situ hybridization. Methods Cell Sci. 21: 91-95.
2. Andras SC, Hartman TPV, Marshall JA, Marchant R, Power JB, Cocking EC, Davey MR (1999). A drop-spreading technique to produce cytoplasm-free mitotic preparations from plants with small chromosomes. Chromosome Res. 7: 641-647.
3. Bass HW, Marshall WF, Sedat JW, Agard DA, Cande WZ (1997). Telomeres cluster de novo before the initiation of synapsis: a threedimensional spatial analysis of telomere positions before and during meiotic prophase. J. Cell Biol. 37: 5-18.
4. Bertao MR, Aguiar-Perecin MLR (2002). Maize somatic chromosome preparation: pretreatments and genotypes for obtention of high index of metaphase accumulation. Caryologia 55: 115-119.
5. Bowler C, Benvenuto1 G, Laflamme P, Molino1 D, Probst AV, Tariq M, Paszkowski J (2004). Chromatin techniques for plant cells. Plant J. 39: 776-789.
6. Busch W, Herrmann RG, Houben A, Martin R (1996). Efficient preparation of plant metaphase spreads. Plant Mol. Biol. Rep. 14: 149-155.
7. Caperta AD, Delgado M, Ressurreição F, Meister A, Jones RN, Viegas W, Houben A (2006). Colchicine-induced polyploidization depends on tubulin polymerization in c-metaphase cells. Protoplasma, 227: 147-153.
8. Doležel J, Cíhalíková J, Weiserová J, Lucretti S (1999). Cell cycle synchronization in plant root meristems. Methods Cell Sci. 21: 95-107.
9. Henegariu O, Heerema NA, Wright LL, Bray-Ward P, Ward DC, Vance GH (2001). Improvements in cytogenetic slides preparation: controlled chromosome spreading, chemical aging and gradual denaturing. Cytometry, 43: 101-109.
10. Houben A, Field BL, Saunders VA (2001). Microdissection and chromosome painting of plant B chromosomes. Methods Cell Sci. 23: 115-124.
11. Leitch AR, Schwarzacher T, Jackson D, Leitch IJ (1994). In situ hybridization: a practical guide. BIOS Scientific, Oxford, UK. Mirzaghaderi G, Karimzadeh G, Hassani HS, Jalali-Javaran M, Baghizadeh A. Cytogenetic analysis of hybrids derived from wheat and Tritipyrum using conventional staining and genomic in situ hybridization. Biol. Plant. (In press).
12. Nishibayashi S, Hayashi Y, Kyozuka J, Shimamoto K (1989). Chromosome variations in protoplast derived calli and in plant regenerated from the calli of cultivated rice (Oryza sativa L.). Jpn. J. Genet. 64: 355-361.
13. Pan WH, Houben A, Schlegel R (1993). Highly effective cell synchronization in plant-roots by hydroxyurea and amiprophosmethyl or colchicine. Genome 36: 387-390.
14. Ryan B, Joiner B (2001). Minitab Handbook. Duxbury Resource Press, California.
15. Shishido R, Ohmido N, Fukui K (2001). Chromosome painting as a tool for rice genetics and breeding. Methods Cell Sci. 23: 125-132.