Improvements in cytogenetic slide preparation: Controlled chromosome spreading, chemical aging and gradual denaturing

Cytometry

Volumn 43, Issue 2, 2001, Pages 101-109

  Henegariu, Octavian ^a   Heerema, Nyla A ^a   Wright, Lisa Lowe ^b   Bray Ward, Patricia ^b   Ward, David C ^c   Vance, Gail H ^a  

^a YALE UNIVERSITY SCHOOL OF MEDICINE   (United States)

^b PARKER HUGHES INSTITUTE   (United States)

^c INDIANA UNIVERSITY   (United States)

Author keywords

Alcohol; CGH; Cytogenetics; FISH technique; G banding; Hybridization; M FISH; Metaphase spreading; Protocol; Slide aging

Indexed keywords

AGING; ANALYTIC METHOD; ARTICLE; CELL CULTURE; CHROMOSOME ANALYSIS; COMPARATIVE GENOMIC HYBRIDIZATION; CYTOGENETICS; DENATURATION; FLUORESCENCE IN SITU HYBRIDIZATION; METAPHASE; PRIORITY JOURNAL; CELL LINE; CENTRIFUGATION; CHROMOSOME BANDING PATTERN; CHROMOSOME PAINTING; COMPARATIVE STUDY; DNA DENATURATION; HUMAN; INSTRUMENTATION; METABOLISM; METHODOLOGY;

DNA;

CELL LINE; CENTRIFUGATION; CHROMOSOME BANDING; CHROMOSOME PAINTING; DNA; DNA, NEOPLASM; HUMANS; IN SITU HYBRIDIZATION, FLUORESCENCE; NUCLEIC ACID DENATURATION; TUMOR CELLS, CULTURED;

EID: 0035241668     PISSN: 01964763     EISSN: None     Source Type: Journal    
DOI: 10.1002/1097-0320(20010201)43:2<101::AID-CYTO1024>3.0.CO;2-8     Document Type: Article

References (16)

1. Peripheral blood culture
2. Ovarian cancer: Protocol for the preparation of banded metaphases from tumor tissue
3. A simple technique for obtaining high quality chromosome preparations from chorionic villus samples using FdU synchronization
4. Harvesting, slide-making and chromosome elongation techniques
5. Preparation, culture and analysis of amniotic fluid samples
6. Chromosome spreading techniques for primary gastrointestinal tumors
7. Preparation and culture of products of conception and other solid tissues for chromosome analysis
8. Banded karyotypes from bone marrow: A clinical useful approach
9. Chorionic villus sampling: Improved method for preparation of karyotypes after short-term incubation
10. Chromosome stains
11. Sequential G- to R-banding for high resolution chromosome analysis
12. Diagnosis of microdeletion syndromes by fluorescence in situ hybridization (FISH)
13. Development of an instrument for chromosome slide preparation
14. Quality control of CGH: Impact of metaphase chromosomes and the dynamic range of hybridization
15. Custom fluorescent-nucleotide synthesis as an alternative method for nucleic acid labeling
16. The spreading of metaphases is a slow process which leads to a stretching of chromosomes