Efficient preparation of plant metaphase spreads

Plant Molecular Biology Reporter

Volumn 14, Issue 2, 1996, Pages 149-155

  Busch, Winfried ^a   Herrmann, Reinhold G ^a   Houben, Andreas ^b   Martin, Regina ^a  

^a UNIVERSITY OF MUNICH   (Germany)

^b LEIBNIZ INSTITUTE OF PLANT GENETICS AND CROP PLANT RESEARCH IPK   (Germany)

Author keywords

Cell cycle synchronization; Drop technique; In situ hybridization; Plant chromosomes

Indexed keywords

EID: 21344444156     PISSN: 07359640     EISSN: None     Source Type: Journal    
DOI: 10.1007/BF02684904     Document Type: Article

References (11)

1. Busch W., R. Martin, R.G. Herrmann. 1994. Sensitivity enhancement of fluorescence in situ hybridization on plant chromosomes. Chromosome Res. 2:15-20.
2. Busch W., R.G. Herrmann, R. Martin. 1995a. Refined physical mapping of the Sec-1 locus in the satellite of chromosome 1R of rye (Secale cereale L.). Genome 38:889-893.
3. Busch W., R. Martin, R.G. Herrmann, U. Hohmann. 1995b. Repeated DNA sequences isolated by microdissection. I. Karyotyping of barley (Hordeum vulgare L.). Genome 38:1082-1090.
4. Busch W., R.G. Herrmann, U. Hohmann. Repeated DNA sequences isolated by microdissection. II. Comparative analysis in Hordeum vulgare and Triticum aestivum. Theor. Appl. Genet., in press.
5. Dolezel J., J. Cihalikova, S. Lucretti. 1992. A high-yield procedure for isolation of metaphase chromosomes from root tips of Vicia faba L. Planta 188:93-98.
6. Houben A., J. Francki, N. Leclerc, W. Ahne. 1995. A computer assisted system combining image analysis and chromosome dissection. Microscopy Res. Techn., in press.
7. Langer-Safer P., M. Levine, D.A. Ward. 1982. An immunological method for mapping genes on Drosophila polytene chromosomes. Proc. Natl. Acad. Sci. USA 79:4381-4385.
8. Martin R., W. Busch, R.G. Herrmann. 1994. Efficient preparation of plant chromosomes for high-resolution scanning electron microscopy. Chromosome Res. 2:411-415.
9. Martin R., W. Busch, R.G. Herrmann, G. Wanner. Changes in chromosomal ultrastructure during the cell cycle. Chromosome Res, in press.
10. Pan W.H., A. Houben, R. Schlegel. 1993. Highly effective cell synchronization in plant roots by hydroxyurea and amiprophosmethyl or colchicine. Genome 36:387-390.
11. Schondelmaier J., R. Martin, A. Jahoor, A. Houben, A. Graner, H.-U. Koop, R.G. Herrmann, C. Jung. 1993. Microdissection and microcloning of the barley (Hordeum vulgare L.) chromosome IHS. Theor. Appl. Genet. 86:629-636.