Modulation of DNA methylation levels sensitizes doxorubicin-resistant breast adenocarcinoma cells to radiation-induced apoptosis

Biochemical and Biophysical Research Communications

Volumn 392, Issue 2, 2010, Pages 113-117

  Luzhna, Lidia ^a   Kovalchuk, Olga ^a  

^a UNIVERSITY OF LETHBRIDGE   (Canada)

Author keywords

Apoptosis; DNA methylation; Radiation resistance; S adenosyl methionine

Indexed keywords

DOXORUBICIN; S ADENOSYLMETHIONINE;

APOPTOSIS; ARTICLE; BREAST ADENOCARCINOMA; CELL STRAIN MCF 7; CONTROLLED STUDY; DNA METHYLATION; EPIGENETICS; HUMAN; HUMAN CELL; IONIZING RADIATION; PRIORITY JOURNAL; RADIATION RESPONSE; RADIOSENSITIVITY;

ADENOCARCINOMA; ANTIBIOTICS, ANTINEOPLASTIC; APOPTOSIS; BREAST NEOPLASMS; CELL LINE, TUMOR; DNA METHYLATION; DOXORUBICIN; DRUG RESISTANCE, NEOPLASM; EPIGENESIS, GENETIC; FEMALE; HUMANS; RADIATION TOLERANCE; S-ADENOSYLMETHIONINE;

EID: 75749126583     PISSN: 0006291X     EISSN: 10902104     Source Type: Journal    
DOI: 10.1016/j.bbrc.2009.12.093     Document Type: Article

References (28)

1. Stavrovskaya A.A. Cellular mechanisms of multidrug resistance of tumor cells. Biochemistry (Mosc.) 65 (2000) 95-106
2. Fojo T. Multiple paths to a drug resistance phenotype: mutations, translocations, deletions and amplification of coding genes or promoter regions, epigenetic changes and microRNAs. Drug Resist. Updat. 10 (2007) 59-67
3. Gottesman M.M. Mechanisms of cancer drug resistance. Annu. Rev. Med. 53 (2002) 615-627
4. Iwasa Y., Nowak M.A., and Michor F. Evolution of resistance during clonal expansion. Genetics 172 (2006) 2557-2566
5. Jaenisch R., and Bird A. Epigenetic regulation of gene expression: how the genome integrates intrinsic and environmental signals. Nat. Genet. 33 Suppl. (2003) 245-254
6. Glasspool R.M., Teodoridis J.M., and Brown R. Epigenetics as a mechanism driving polygenic clinical drug resistance. Br. J. Cancer 94 (2006) 1087-1092
7. Brown R., and Glasspool R. Epigenetic modulation of resistance to chemotherapy?. Ann. Oncol. 18 (2007) 1429-1430
8. Chekhun V.F., Kulik G.I., Yurchenko O.V., Tryndyak V.P., Todor I.N., Luniv L.S., Tregubova N.A., Pryzimirska T.V., Montgomery B., Rusetskaya N.V., and Pogribny I.P. Role of DNA hypomethylation in the development of the resistance to doxorubicin in human MCF-7 breast adenocarcinoma cells. Cancer Lett. 231 (2006) 87-93
9. Baker E.K., and El-Osta A. The rise of DNA methylation and the importance of chromatin on multidrug resistance in cancer. Exp. Cell Res. 290 (2003) 177-194
10. Duesberg P., Li R., Sachs R., Fabarius A., Upender M.B., and Hehlmann R. Cancer drug resistance: the central role of the karyotype. Drug Resist. Updat. 10 (2007) 51-58
11. Kovalchuk O., Filkowski J., Meservy J., Ilnytskyy Y., Tryndyak V.P., Chekhun V.F., and Pogribny I.P. Involvement of microRNA-451 in resistance of the MCF-7 breast cancer cells to chemotherapeutic drug doxorubicin. Mol. Cancer Ther. 7 (2008) 2152-2159
12. Teodoridis J.M., Strathdee G., Plumb J.A., and Brown R. CpG-island methylation and epigenetic control of resistance to chemotherapy. Biochem. Soc. Trans. 32 (2004) 916-917
13. Chekhun V.F., Lukyanova N.Y., Kovalchuk O., Tryndyak V.P., and Pogribny I.P. Epigenetic profiling of multidrug-resistant human MCF-7 breast adenocarcinoma cells reveals novel hyper- and hypomethylated targets. Mol. Cancer Ther. 6 (2007) 1089-1098
14. T. Scandinavian Breast Group, Tanner M., Isola J., Wiklund T., Erikstein B., Kellokumpu-Lehtinen P., Malmstrom P., Wilking N., Nilsson J., and Bergh J. Topoisomerase IIalpha gene amplification predicts favorable treatment response to tailored and dose-escalated anthracycline-based adjuvant chemotherapy in HER-2/neu-amplified breast cancer: Scandinavian Breast Group Trial 9401. J. Clin. Oncol. 24 (2006) 2428-2436
15. Dean-Colomb W., and Esteva F.J. Emerging agents in the treatment of anthracycline- and taxane-refractory metastatic breast cancer. Semin. Oncol. 35 (2008) S31-S38 (quiz S40)
16. Di Leo A., Gancberg D., Larsimont D., Tanner M., Jarvinen T., Rouas G., Dolci S., Leroy J.Y., Paesmans M., Isola J., and Piccart M.J. HER-2 amplification and topoisomerase IIalpha gene aberrations as predictive markers in node-positive breast cancer patients randomly treated either with an anthracycline-based therapy or with cyclophosphamide, methotrexate, and 5-fluorouracil. Clin. Cancer Res. 8 (2002) 1107-1116
17. Fornari F.A., Randolph J.K., Yalowich J.C., Ritke M.K., and Gewirtz D.A. Interference by doxorubicin with DNA unwinding in MCF-7 breast tumor cells. Mol. Pharmacol. 45 (1994) 649-656
18. Frederick C.A., Williams L.D., Ughetto G., van der Marel G.A., van Boom J.H., Rich A., and Wang A.H. Structural comparison of anticancer drug-DNA complexes: adriamycin and daunomycin. Biochemistry 29 (1990) 2538-2549
19. Detich N., Hamm S., Just G., Knox J.D., and Szyf M. The methyl donor S-adenosylmethionine inhibits active demethylation of DNA: a candidate novel mechanism for the pharmacological effects of S-adenosylmethionine. J. Biol. Chem. 278 (2003) 20812-20820
20. Pogribny I., Yi P., and James S.J. A sensitive new method for rapid detection of abnormal methylation patterns in global DNA and within CpG islands. Biochem. Biophys. Res. Commun. 7 (1999) 624-628
21. K.A. Camphausen, R.C. Lawrence, Principles of radiation therapy, in: R. Pazdur, L.D. Wagman, K.A. Camphausen, W.J. Hoskins (Eds.), Cancer Management: A Multidisciplinary Approach, eleventh ed., 2008. Available from: .
22. Vermes I., Haanen C., Steffens-Nakken H., and Reutelingsperger C. A novel assay for apoptosis. Flow cytometric detection of phosphatidylserine expression on early apoptotic cells using fluorescein labelled Annexin V. J. Immunol. Methods 184 (1995) 39-51
23. Szyf M., Pakneshan P., and Rabbani S.A. DNA demethylation and cancer: therapeutic implications. Cancer Lett. 211 (2004) 133-143
24. Yi P., Melnyk S., Pogribna M., Pogribny I.P., Hine R.J., and James S.J. Increase in plasma homocysteine associated with parallel increases in plasma S-adenosylhomocysteine and lymphocyte DNA hypomethylation. J. Biol. Chem. 275 (2000) 29318-29323
25. Castro R., Rivera I., Martins C., Struys E.A., Jansen E.E., and Clode N. Intracellular S-adenosylhomocysteine increased levels are associated with DNA hypomethylation in HUVEC. J. Mol. Med. 83 (2005) 831-836
26. Hoffman D.R., Cornatzer W.E., and Duerre J.A. Relationship between tissue levels of S-adenosylmethionine, S-adenylhomocysteine, and transmethylation reactions. Can. J. Biochem. 57 (1979) 56-65
27. Pakneshan P., Szyf M., Farias-Eisner R., and Rabbani S.A. Reversal of the hypomethylation status of urokinase (uPA) promoter blocks breast cancer growth and metastasis. J. Biol. Chem. 23 (2004) 31735-31744
28. Shukeir N., Pakneshan P., Chen G., Szyf M., and Rabbani S.A. Alteration of the methylation status of tumor-promoting genes decreases prostate cancer cell invasiveness in tumorigenesis in vitro and in vivo. Cancer Res. 66 (2006) 9202-9210